Glycine oxidase
Details
- Name
- Glycine oxidase
- Synonyms
- 1.4.3.19
- GO
- goxB
- yjbR
- Gene Name
- thiO
- Organism
- Bacillus subtilis (strain 168)
- Amino acid sequence
>lcl|BSEQ0016486|Glycine oxidase MKRHYEAVVIGGGIIGSAIAYYLAKENKNTALFESGTMGGRTTSAAAGMLGAHAECEERD AFFDFAMHSQRLYKGLGEELYALSGVDIRQHNGGMFKLAFSEEDVLQLRQMDDLDSVSWY SKEEVLEKEPYASGDIFGASFIQDDVHVEPYFVCKAYVKAAKMLGAEIFEHTPVLHVERD GEALFIKTPSGDVWANHVVVASGVWSGMFFKQLGLNNAFLPVKGECLSVWNDDIPLTKTL YHDHCYIVPRKSGRLVVGATMKPGDWSETPDLGGLESVMKKAKTMLPAIQNMKVDRFWAG LRPGTKDGKPYIGRHPEDSRILFAAGHFRNGILLAPATGALISDLIMNKEVNQDWLHAFR IDRKEAVQI
- Number of residues
- 369
- Molecular Weight
- 40936.53
- Theoretical pI
- 6.32
- GO Classification
- Functionsflavin adenine dinucleotide binding / glycine oxidase activityProcessesresponse to herbicide / thiamine biosynthetic process / thiamine diphosphate biosynthetic processComponentscytoplasm
- General Function
- Glycine oxidase activity
- Specific Function
- Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine. Does not act on L-amino acids and other D-amino acids. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.
- Pfam Domain Function
- DAO (PF01266)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0016487|Glycine oxidase (thiO) ATGAAAAGGCATTATGAAGCAGTGGTGATTGGAGGCGGAATTATCGGTTCCGCAATTGCT TATTATTTGGCAAAGGAAAACAAAAACACCGCATTGTTTGAAAGCGGAACAATGGGCGGC AGAACGACAAGTGCCGCTGCCGGAATGCTGGGCGCCCATGCCGAATGCGAGGAACGTGAC GCGTTTTTTGATTTCGCTATGCACAGTCAGCGTCTGTACAAAGGTCTTGGAGAAGAGCTT TATGCATTATCCGGTGTGGATATCAGGCAGCATAACGGCGGTATGTTTAAGCTTGCATTT TCTGAAGAAGATGTGCTGCAGCTGAGACAGATGGACGATTTGGACTCTGTCAGCTGGTAT TCAAAAGAAGAGGTGTTAGAAAAAGAGCCGTATGCGTCTGGTGACATCTTTGGTGCATCT TTTATTCAGGATGATGTGCATGTGGAGCCTTATTTTGTTTGCAAGGCATATGTGAAAGCA GCAAAAATGCTTGGGGCGGAGATTTTTGAGCATACGCCCGTCCTGCATGTCGAACGTGAC GGTGAAGCCCTGTTCATCAAGACCCCTAGCGGAGACGTATGGGCTAATCATGTTGTCGTT GCCAGCGGGGTGTGGAGCGGAATGTTTTTTAAACAGCTTGGACTGAACAATGCTTTTCTC CCTGTAAAAGGGGAGTGCCTGTCCGTTTGGAATGATGATATCCCGCTGACAAAAACGCTT TACCATGATCACTGCTATATCGTACCGAGAAAAAGCGGAAGACTGGTTGTCGGCGCGACA ATGAAGCCGGGGGACTGGAGTGAAACACCGGATCTTGGCGGATTGGAATCTGTTATGAAA AAAGCAAAAACGATGCTGCCGGCTATACAGAATATGAAGGTGGATCGTTTTTGGGCGGGA CTCCGTCCGGGAACAAAGGATGGAAAACCGTACATCGGCAGACATCCTGAGGACAGCCGT ATTTTATTTGCGGCTGGCCATTTCAGAAACGGGATCCTGCTTGCTCCCGCAACGGGCGCT TTGATCAGTGATCTCATCATGAATAAAGAGGTCAACCAAGACTGGCTGCACGCATTCCGA ATTGATCGCAAGGAGGCGGTTCAGATATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID O31616 UniProtKB Entry Name GLOX_BACSU GenBank Protein ID 2633520 GenBank Gene ID Z99110 - General References
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- Job V, Marcone GL, Pilone MS, Pollegioni L: Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein. J Biol Chem. 2002 Mar 1;277(9):6985-93. Epub 2001 Dec 13. [Article]
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