D-lactate dehydrogenase

Details

Name

D-lactate dehydrogenase

Synonyms

• 1.1.1.28
• Respiratory D-lactate dehydrogenase

Gene Name

dld

Organism

Escherichia coli (strain K12)

Amino acid sequence

>lcl|BSEQ0036952|D-lactate dehydrogenase
MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR
VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP
GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN
EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP
ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF
ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH
FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF
VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ
LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET
LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE

Number of residues

571

Molecular Weight

64611.82

Theoretical pI

6.65

GO Classification

Functions

D-lactate dehydrogenase activity / electron carrier activity / flavin adenine dinucleotide binding / NAD binding / oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor

Processes

aerobic respiration / anaerobic respiration / lactate oxidation / respiratory electron transport chain / transmembrane transport

Components

extrinsic component of cytoplasmic side of plasma membrane / integral component of plasma membrane / plasma membrane

General Function

Oxidoreductase activity, acting on the ch-oh group of donors, quinone or similar compound as acceptor

Specific Function

First component of the membrane-bound D-lactate oxidase, which is believed to play an important role in the energization of the active transport of a variety of sugars and amino acids.

Pfam Domain Function

• FAD_binding_4 (PF01565)
• Lact-deh-memb (PF09330)

Transmembrane Regions

Not Available

Cellular Location

Cell membrane

Gene sequence

>lcl|BSEQ0009944|D-lactate dehydrogenase (dld)
ATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTG
GGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGT
TCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGG
GTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGC
CTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGC
ACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCG
GGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCA
GTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGC
GGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAAT
GAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAG
CAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGT
CGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCC
GCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG
GCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGTTTTAT
ATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTC
GAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGCGGAAAAA
TACGGCAAAGACACCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTC
TTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCAT
TTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGC
ATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGC
GTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTT
GTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCA
GCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATC
GCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAG
CTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATA
GTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAG
CGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACG
TTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAA
ACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAA

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	P06149
UniProtKB Entry Name	DLD_ECOLI
GenBank Protein ID	145753
GenBank Gene ID	M10038

General References

1. Campbell HD, Rogers BL, Young IG: Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli. Eur J Biochem. 1984 Oct 15;144(2):367-73. [Article]
2. Rule GS, Pratt EA, Chin CC, Wold F, Ho C: Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli. J Bacteriol. 1985 Mar;161(3):1059-68. [Article]
3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
5. VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
6. Dym O, Pratt EA, Ho C, Eisenberg D: The crystal structure of D-lactate dehydrogenase, a peripheral membrane respiratory enzyme. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9413-8. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB00756	Hexachlorophene	approved, withdrawn	yes	inhibitor	Details
DB03147	Flavin adenine dinucleotide	approved	unknown		Details