Evolved beta-galactosidase subunit alpha
Details
- Name
- Evolved beta-galactosidase subunit alpha
- Synonyms
- 3.2.1.23
- Beta-gal
- Lactase
- Gene Name
- ebgA
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0000232|Evolved beta-galactosidase subunit alpha MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPLSGQWNFHFFDHPLQVP EAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLS DGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMVKTGDNLLCVRVMQW ADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATLSCEVVLENLAA SPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTL KDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKD LQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQW EKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAMYHAAKALDDTRLVHYEEDRDA EVVDIISTMYTRVPLMNEFGEYPHPKPRIICEYAHAMGNGPGGLTEYQNVFYKHDCIQGH YVWEWCDHGIQAQDDHGNVWYKFGGDYGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIA PVKIHARDLTRGELKVENKLWFTTLDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQ ITLPQLDAREAFLNITVTKDSRTRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLE DDRLSCTVRGYNFAITFSKMSGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQ PNHLQIMQEHLRDFAVEQSDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSG ERYGDYPHIIPCIGFTMGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENY PFPQNNGNRQHVRWTALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDI TLNLDHQLLGLGSNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFF STNLHSENKQ
- Number of residues
- 1030
- Molecular Weight
- 117878.225
- Theoretical pI
- 5.25
- GO Classification
- Functionsbeta-galactosidase activity / carbohydrate bindingProcessescarbohydrate metabolic processComponentsbeta-galactosidase complex
- General Function
- Carbohydrate binding
- Specific Function
- The wild-type enzyme is an ineffective lactase. Two classes of point mutations dramatically improve activity of the enzyme.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0010167|Evolved beta-galactosidase subunit alpha (ebgA) ATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTAC TTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTG TTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCA GAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGG CAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGAT GTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGC GACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAA GTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTT GACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGG GCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGAT GTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTT GACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCC TCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGC AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC GAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG AAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATC AAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAAC CGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAT CTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGAT CCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTC GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGG GAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCG ATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAC CATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT GAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGT GAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGA CCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCAT TATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGG TATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGT TTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCG CCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTG TGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACG CTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAG ATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGAT TCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAG GAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAA GACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATG AGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAG ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAA CCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAT GGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATG CGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGC GAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAAC GGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGC CAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAT CCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGC CACGGTAACGGTCTGCTGGTGGTTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTAT ACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATC ACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTG CTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTT TCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCTTT TCCACGAATTTGCACAGCGAGAATAAGCAATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P06864 UniProtKB Entry Name BGA2_ECOLI GenBank Protein ID 145821 GenBank Gene ID M64441 - General References
- Hall BG, Betts PW, Wootton JC: DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor genes. Genetics. 1989 Dec;123(4):635-48. [Article]
- Stokes HW, Betts PW, Hall BG: Sequence of the ebgA gene of Escherichia coli: comparison with the lacZ gene. Mol Biol Evol. 1985 Nov;2(6):469-77. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G 3rd, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL: Escherichia coli K-12: a cooperatively developed annotation snapshot--2005. Nucleic Acids Res. 2006 Jan 5;34(1):1-9. Print 2006. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Fowler AV, Smith PJ: The active site regions of lacZ and ebg beta-galactosidases are homologous. J Biol Chem. 1983 Sep 10;258(17):10204-7. [Article]
- Elliott AC, K S, Sinnott ML, Smith PJ, Bommuswamy J, Guo Z, Hall BG, Zhang Y: The catalytic consequences of experimental evolution. Studies on the subunit structure of the second (ebg) beta-galactosidase of Escherichia coli, and on catalysis by ebgab, an experimental evolvant containing two amino acid substitutions. Biochem J. 1992 Feb 15;282 ( Pt 1):155-64. [Article]