Phosphoribosylglycinamide formyltransferase
Details
- Name
- Phosphoribosylglycinamide formyltransferase
- Synonyms
- 2.1.2.2
- 5'-phosphoribosylglycinamide transformylase
- GAR transformylase
- GART
- Gene Name
- purN
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0020533|Phosphoribosylglycinamide formyltransferase MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLERARQAGIATHTLIASA FDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAGRLLNIHPSLLPKYPGLHT HRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGDSEDDITARVQTQEHAIYPLV ISWFADGRLKMHENAAWLDGQRLPPQGYAADE
- Number of residues
- 212
- Molecular Weight
- 23238.065
- Theoretical pI
- 5.74
- GO Classification
- Functionsmethyltransferase activity / phosphoribosylglycinamide formyltransferase activityProcesses'de novo' IMP biosynthetic process / cellular response to DNA damage stimulusComponentscytosol
- General Function
- Phosphoribosylglycinamide formyltransferase activity
- Specific Function
- Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.
- Pfam Domain Function
- Formyl_trans_N (PF00551)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0020534|Phosphoribosylglycinamide formyltransferase (purN) ATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCC TGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCG TTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCG TTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCC GATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTAT GCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACC CATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCGGTGCATTTCGTCACC GATGAACTGGACGGTGGCCCGGTTATTTTACAGGCGAAAGTCCCGGTATTTGCTGGTGAT TCGGAAGATGACATCACCGCCCGCGTGCAAACCCAGGAACACGCCATTTATCCACTGGTG ATTAGCTGGTTTGCCGATGGTCGTCTGAAAATGCACGAAAACGCCGCGTGGCTGGATGGT CAACGTCTGCCGCCGCAGGGCTACGCTGCCGACGAGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P08179 UniProtKB Entry Name PUR3_ECOLI GenBank Protein ID 147425 GenBank Gene ID M13747 - General References
- Smith JM, Daum HA 3rd: Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12. J Biol Chem. 1987 Aug 5;262(22):10565-9. [Article]
- Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Pupo GM, Lan R, Reeves PR: Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics. Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10567-72. [Article]
- Inglese J, Johnson DL, Shiau A, Smith JM, Benkovic SJ: Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase. Biochemistry. 1990 Feb 13;29(6):1436-43. [Article]
- Dev IK, Harvey RJ: N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli. J Biol Chem. 1978 Jun 25;253(12):4242-4. [Article]
- Inglese J, Smith JM, Benkovic SJ: Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli. Biochemistry. 1990 Jul 17;29(28):6678-87. [Article]
- Warren MS, Marolewski AE, Benkovic SJ: A rapid screen of active site mutants in glycinamide ribonucleotide transformylase. Biochemistry. 1996 Jul 9;35(27):8855-62. [Article]
- Shim JH, Benkovic SJ: Catalytic mechanism of Escherichia coli glycinamide ribonucleotide transformylase probed by site-directed mutagenesis and pH-dependent studies. Biochemistry. 1999 Aug 3;38(31):10024-31. [Article]
- Chen P, Schulze-Gahmen U, Stura EA, Inglese J, Johnson DL, Marolewski A, Benkovic SJ, Wilson IA: Crystal structure of glycinamide ribonucleotide transformylase from Escherichia coli at 3.0 A resolution. A target enzyme for chemotherapy. J Mol Biol. 1992 Sep 5;227(1):283-92. [Article]
- Almassy RJ, Janson CA, Kan CC, Hostomska Z: Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6114-8. [Article]
- Su Y, Yamashita MM, Greasley SE, Mullen CA, Shim JH, Jennings PA, Benkovic SJ, Wilson IA: A pH-dependent stabilization of an active site loop observed from low and high pH crystal structures of mutant monomeric glycinamide ribonucleotide transformylase at 1.8 to 1.9 A. J Mol Biol. 1998 Aug 21;281(3):485-99. [Article]
- Greasley SE, Yamashita MM, Cai H, Benkovic SJ, Boger DL, Wilson IA: New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid. Biochemistry. 1999 Dec 21;38(51):16783-93. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB02236 Glycinamide Ribonucleotide experimental unknown Details DB02540 10-formyl-5,8,10-trideazafolic acid experimental unknown Details DB02794 (2S)-2-({4-[(2S)-1-(2-Amino-4-oxo-1,4-dihydro-6-quinazolinyl)-3-{[2-({(2R,3R,4S,5R)-3,4-dihydroxy-5-[(phosphonooxy)methyl]tetrahydro-2-furanyl}amino)-2-oxoethyl]amino}-2-hydroxy-2-propanyl]benzoyl}ami no)pentanedioic acid experimental unknown Details DB04264 (10R)-10-formyl-5,8,10-trideazafolic acid experimental unknown Details