UDP-glucose 4-epimerase

Details

Name
UDP-glucose 4-epimerase
Synonyms
  • 5.1.3.2
  • Galactowaldenase
  • galD
  • UDP-galactose 4-epimerase
Gene Name
galE
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016603|UDP-glucose 4-epimerase
MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGKHPTFVEGDIR
NEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVNGTLRLISAMRAANVKNFI
FSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQILTDLQKAQPDWSIALLRYFNP
VGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLA
DGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYW
ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD
Number of residues
338
Molecular Weight
37264.875
Theoretical pI
6.32
GO Classification
Functions
identical protein binding / NAD+ binding / racemase and epimerase activity, acting on carbohydrates and derivatives / UDP-glucose 4-epimerase activity
Processes
carbohydrate metabolic process / colanic acid biosynthetic process / galactose catabolic process via UDP-galactose / galactose metabolic process
Components
cytoplasm / cytosol
General Function
Udp-glucose 4-epimerase activity
Specific Function
Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. It is only active on UDP-galactose and UDP-glucose.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016604|UDP-glucose 4-epimerase (galE)
ATGAGAGTTCTGGTTACCGGTGGTAGCGGTTACATTGGAAGTCATACCTGTGTGCAATTA
CTGCAAAACGGTCATGATGTCATCATTCTTGATAACCTCTGTAACAGTAAGCGCAGCGTA
CTGCCTGTTATCGAGCGTTTAGGCGGCAAACATCCAACGTTTGTTGAAGGCGATATTCGT
AACGAAGCGTTGATGACCGAGATCCTGCACGATCACGCTATCGACACCGTGATCCACTTC
GCCGGGCTGAAAGCCGTGGGCGAATCGGTACAAAAACCGCTGGAATATTACGACAACAAT
GTCAACGGCACTCTGCGCCTGATTAGCGCCATGCGCGCCGCTAACGTCAAAAACTTTATT
TTTAGCTCCTCCGCCACCGTTTATGGCGATCAGCCCAAAATTCCATACGTTGAAAGCTTC
CCGACCGGCACACCGCAAAGCCCTTACGGCAAAAGCAAGCTGATGGTGGAACAGATCCTC
ACCGATCTGCAAAAAGCCCAGCCGGACTGGAGCATTGCCCTGCTGCGCTACTTCAACCCG
GTTGGCGCGCATCCGTCGGGCGATATGGGCGAAGATCCGCAAGGCATTCCGAATAACCTG
ATGCCATACATCGCCCAGGTTGCTGTAGGCCGTCGCGACTCGCTGGCGATTTTTGGTAAC
GATTATCCGACCGAAGATGGTACTGGCGTACGCGATTACATCCACGTAATGGATCTGGCG
GACGGTCACGTCGTGGCGATGGAAAAACTGGCGAACAAGCCAGGCGTACACATCTACAAC
CTCGGCGCTGGCGTAGGCAACAGCGTGCTGGACGTGGTTAATGCCTTCAGCAAAGCCTGC
GGCAAACCGGTTAATTATCATTTTGCACCGCGTCGCGAGGGCGACCTTCCGGCCTACTGG
GCGGACGCCAGCAAAGCCGACCGTGAACTGAACTGGCGCGTAACGCGCACACTCGATGAA
ATGGCGCAGGACACCTGGCACTGGCAGTCACGCCATCCACAGGGATATCCCGATTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP09147
UniProtKB Entry NameGALE_ECOLI
GenBank Protein ID41523
GenBank Gene IDX06226
General References
  1. Lemaire HG, Muller-Hill B: Nucleotide sequences of the gal E gene and the gal T gene of E. coli. Nucleic Acids Res. 1986 Oct 10;14(19):7705-11. [Article]
  2. Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Bernardi F, Bernardi A: Completed sequence of pKG1800, a vector for determination of transcription terminators. DNA Seq. 1990;1(2):147-50. [Article]
  6. Walkenhorst HM, Hemschemeier SK, Eichenlaub R: Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene. Microbiol Res. 1995 Nov;150(4):347-61. [Article]
  7. Busby S, Dreyfus M: Segment-specific mutagenesis of the regulatory region in the Escherichia coli galactose operon: isolation of mutations reducing the initiation of transcription and translation. Gene. 1983 Jan-Feb;21(1-2):121-31. [Article]
  8. WILSON DB, HOGNESS DS: THE ENZYMES OF THE GALACTOSE OPERON IN ESCHERICHIA COLI. I. PURIFICATION AND CHARACTERIZATION OF URIDINE DIPHOSPHOGALACTOSE 4-EPIMERASE. J Biol Chem. 1964 Aug;239:2469-81. [Article]
  9. Wilson DB, Hogness DS: The enzymes of the galactose operon in Escherichia coli. II. The subunits of uridine diphosphogalactose 4-epimerase. J Biol Chem. 1969 Apr 25;244(8):2132-6. [Article]
  10. Swanson BA, Frey PA: Identification of lysine 153 as a functionally important residue in UDP-galactose 4-epimerase from Escherichia coli. Biochemistry. 1993 Dec 7;32(48):13231-6. [Article]
  11. Liu Y, Vanhooke JL, Frey PA: UDP-galactose 4-epimerase: NAD+ content and a charge-transfer band associated with the substrate-induced conformational transition. Biochemistry. 1996 Jun 11;35(23):7615-20. [Article]
  12. Bauer AJ, Rayment I, Frey PA, Holden HM: The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolution. Proteins. 1992 Apr;12(4):372-81. [Article]
  13. Thoden JB, Frey PA, Holden HM: Crystal structures of the oxidized and reduced forms of UDP-galactose 4-epimerase isolated from Escherichia coli. Biochemistry. 1996 Feb 27;35(8):2557-66. [Article]
  14. Thoden JB, Frey PA, Holden HM: Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism. Biochemistry. 1996 Apr 23;35(16):5137-44. [Article]
  15. Thoden JB, Frey PA, Holden HM: High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol. Protein Sci. 1996 Nov;5(11):2149-61. [Article]
  16. Thoden JB, Hegeman AD, Wesenberg G, Chapeau MC, Frey PA, Holden HM: Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli. Biochemistry. 1997 May 27;36(21):6294-304. [Article]
  17. Liu Y, Thoden JB, Kim J, Berger E, Gulick AM, Ruzicka FJ, Holden HM, Frey PA: Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coli. Biochemistry. 1997 Sep 2;36(35):10675-84. [Article]
  18. Thoden JB, Gulick AM, Holden HM: Molecular structures of the S124A, S124T, and S124V site-directed mutants of UDP-galactose 4-epimerase from Escherichia coli. Biochemistry. 1997 Sep 2;36(35):10685-95. [Article]
  19. Thoden JB, Holden HM: Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coli. Biochemistry. 1998 Aug 18;37(33):11469-77. [Article]
  20. Thoden JB, Henderson JM, Fridovich-Keil JL, Holden HM: Structural analysis of the Y299C mutant of Escherichia coli UDP-galactose 4-epimerase. Teaching an old dog new tricks. J Biol Chem. 2002 Jul 26;277(30):27528-34. Epub 2002 May 17. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB02421Uridine-5'-diphosphate-mannoseexperimentalunknownDetails
DB02790Phenyl-uridine-5'-diphosphateexperimentalunknownDetails
DB03397Uridine-Diphosphate-N-AcetylglucosamineexperimentalunknownDetails
DB03435Uridine-5'-DiphosphateexperimentalunknownDetails
DB04097Uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-D-galactoseexperimentalunknownDetails
DB01861Uridine diphosphate glucoseexperimentalunknownDetails