Bifunctional protein PutA
Details
- Name
- Bifunctional protein PutA
- Synonyms
- poaA
- Gene Name
- putA
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0020566|Bifunctional protein PutA MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQLENSDTLPELPALLSGA ANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRPETEAVSMLLEQARLPQPV AEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQEGVALMCLAEALLRIPDKAT RDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGKLVSTHNEASLSRSLNRIIGKSG EPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLEEKGFRYSYDMLGEAALTAADAQAY MVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEELYPRLKSLTLLA RQYDIGINIDAEESDRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPLVIDYLIDL ATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLI YPQFATHNAHTLAAIYQLAGQNYYPGQYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIY APVGTHETLLAYLVRRLLENGANTSFVNRIADTSLPLDELVADPVTAVEKLAQQEGQTGL PHPKIPLPRDLYGHGRDNSAGLDLANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEM SPVINPAEPKDIVGYVREATPREVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQ MQQLIGILVREAGKTFSNAIAEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNF PLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGA QLTGDDRVRGVMFTGSTEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQ VVVDVLASAFDSAGQRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVI DSEAKANIERHIQTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVF GPVLHVVRYNRNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVG AVVGVQPFGGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAKYPVDAQLKAALT QPLNALREWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDE QDALTQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHG DSDQLRALCEAVAARDGTIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
- Number of residues
- 1320
- Molecular Weight
- 143813.665
- Theoretical pI
- 5.84
- GO Classification
- Functions1-pyrroline-5-carboxylate dehydrogenase activity / aldehyde dehydrogenase (NAD) activity / bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding / DNA binding / flavin adenine dinucleotide binding / proline dehydrogenase activity / sequence-specific DNA binding / transcriptional repressor activity, bacterial-type RNA polymerase core promoter proximal region sequence-specific bindingProcessesnegative regulation of transcription, DNA-templated / proline biosynthetic process / proline catabolic process to glutamate / transcription, DNA-templatedComponentscytoplasmic side of plasma membrane
- General Function
- Transcriptional repressor activity, bacterial-type rna polymerase core promoter proximal region sequence-specific binding
- Specific Function
- Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0020567|Bifunctional protein PutA (putA) ATGGGAACCACCACCATGGGGGTTAAGCTGGACGACGCGACGCGTGAGCGTATTAAGTCT GCCGCGACACGTATCGATCGCACACCACACTGGTTAATTAAGCAGGCGATTTTTTCTTAT CTCGAACAACTGGAAAACAGCGATACTCTGCCGGAGCTACCTGCGCTGCTTTCTGGCGCG GCCAATGAGAGCGATGAAGCACCGACTCCGGCAGAGGAACCACACCAGCCATTCCTCGAC TTTGCCGAGCAAATATTGCCCCAGTCGGTTTCCCGCGCCGCGATCACCGCGGCCTATCGC CGCCCGGAAACCGAAGCGGTTTCTATGCTGCTGGAACAAGCCCGCCTGCCGCAGCCAGTT GCTGAACAGGCGCACAAACTGGCGTATCAGCTGGCCGATAAACTGCGTAATCAAAAAAAT GCCAGTGGTCGCGCAGGTATGGTCCAGGGGTTATTGCAGGAGTTTTCGCTGTCATCGCAG GAAGGCGTGGCGCTGATGTGTCTGGCGGAAGCGTTGTTGCGTATTCCCGACAAAGCCACC CGCGACGCGTTAATTCGCGACAAAATCAGCAACGGTAACTGGCAGTCACACATTGGTCGT AGCCCGTCACTGTTTGTTAATGCCGCCACCTGGGGGCTGCTGTTTACTGGCAAACTGGTT TCCACCCATAACGAAGCCAGCCTCTCCCGCTCGCTGAACCGCATTATCGGTAAAAGCGGT GAACCGCTGATCCGCAAAGGTGTGGATATGGCGATGCGCCTGATGGGTGAGCAGTTCGTC ACTGGCGAAACCATCGCGGAAGCGTTAGCCAATGCCCGCAAGCTGGAAGAGAAAGGTTTC CGTTACTCTTACGATATGCTGGGCGAAGCCGCGCTGACCGCCGCAGATGCACAGGCGTAT ATGGTTTCCTATCAGCAGGCGATTCACGCCATCGGTAAAGCGTCTAACGGTCGTGGCATC TATGAAGGGCCGGGCATTTCAATCAAACTGTCGGCGCTGCATCCGCGTTATAGCCGCGCC CAGTATGACCGGGTAATGGAAGAGCTTTACCCGCGTCTGAAATCACTCACCCTGCTGGCG CGTCAGTACGATATTGGTATCAACATTGACGCCGAAGAGTCCGATCGCCTGGAGATCTCC CTCGATCTGCTGGAAAAACTCTGTTTCGAGCCGGAACTGGCAGGCTGGAACGGCATCGGT TTTGTTATTCAGGCTTATCAAAAACGCTGCCCGTTGGTGATCGATTACCTGATTGATCTC GCCACCCGCAGCCGTCGCCGTCTGATGATTCGCCTGGTGAAAGGCGCGTACTGGGATAGT GAAATTAAGCGTGCGCAGATGGACGGCCTTGAAGGTTATCCGGTTTATACCCGCAAGGTG TATACCGACGTTTCTTATCTCGCCTGTGCGAAAAAGCTGCTGGCGGTGCCGAATCTAATC TACCCGCAGTTCGCGACGCACAACGCCCATACGCTGGCGGCGATTTATCAACTGGCGGGG CAGAACTACTACCCGGGTCAGTACGAGTTCCAGTGCCTGCATGGTATGGGCGAGCCACTG TATGAGCAGGTCACCGGGAAAGTTGCCGACGGCAAACTTAACCGTCCGTGTCGTATTTAT GCTCCGGTTGGCACACATGAAACGCTGTTGGCGTATCTGGTGCGTCGCCTGCTGGAAAAC GGTGCTAACACCTCGTTTGTTAACCGTATTGCCGACACCTCTTTGCCACTGGATGAACTG GTCGCCGATCCGGTCACTGCTGTAGAAAAACTGGCGCAACAGGAAGGGCAAACTGGATTA CCGCATCCGAAAATTCCCCTGCCGCGCGATCTTTACGGTCACGGGCGCGACAACTCGGCA GGGCTGGATCTCGCTAACGAACACCGCCTGGCCTCGCTCTCCTCTGCCCTGCTCAATAGT GCACTGCAAAAATGGCAGGCCTTGCCAATGCTGGAACAACCGGTAGCGGCAGGTGAGATG TCGCCCGTTATTAACCCTGCGGAACCGAAAGATATTGTGGGCTATGTGCGTGAAGCCACG CCGCGTGAAGTAGAACAGGCGCTGGAAAGTGCGGTTAATAACGCGCCAATCTGGTTTGCC ACGCCTCCGGCTGAACGCGCAGCGATTTTGCACCGCGCTGCCGTGCTGATGGAAAGCCAG ATGCAGCAACTGATTGGTATTCTGGTGCGTGAGGCCGGAAAAACCTTCAGTAACGCCATT GCCGAAGTGCGCGAAGCGGTCGATTTTCTCCACTACTACGCCGGACAGGTGCGGGATGAT TTCGCTAACGAAACCCACCGTCCATTAGGGCCTGTGGTGTGTATCAGTCCGTGGAACTTC CCGCTGGCTATTTTCACCGGGCAGATCGCCGCCGCACTGGCGGCAGGTAACAGCGTGCTG GCAAAACCGGCAGAACAAACGCCGCTGATTGCCGCGCAAGGGATCGCCATTTTGCTGGAA GCGGGTGTACCGCCAGGCGTGGTGCAATTGCTGCCAGGTCGGGGTGAAACCGTGGGCGCG CAACTGACGGGTGATGATCGCGTGCGCGGGGTGATGTTTACCGGTTCAACCGAAGTCGCT ACGTTACTGCAGCGCAATATCGCCAGCCGCCTGGACGCTCAGGGTCGCCCTATTCCGCTC ATCGCTGAAACCGGCGGCATGAACGCGATGATTGTCGATTCTTCAGCACTGACCGAACAG GTCGTCGTGGATGTACTGGCCTCGGCGTTCGACAGTGCGGGTCAGCGTTGTTCGGCGCTG CGCGTGCTGTGCCTGCAAGATGAGATTGCCGACCACACGTTGAAAATGCTGCGCGGCGCA ATGGCCGAATGCCGGATGGGTAATCCGGGTCGCCTGACCACCGATATCGGTCCAGTGATT GATAGCGAAGCGAAAGCCAATATTGAGCGCCATATTCAGACCATGCGTAGCAAAGGCCGT CCGGTGTTCCAGGCGGTGCGGGAAAACAGCGAAGATGCCCGTGAATGGCAAAGCGGCACC TTTGTCGCCCCGACGCTGATCGAACTGGATGACTTTGCCGAATTGCAAAAAGAGGTCTTT GGTCCGGTGCTGCATGTGGTGCGTTACAACCGTAACCAGCTACCAGAGCTGATCGAGCAG ATTAACGCTTCCGGTTATGGTCTGACGCTTGGCGTCCATACGCGCATTGATGAAACCATC GCCCAGGTCACTGGCTCGGCCCATGTTGGTAACCTGTATGTTAACCGTAATATGGTGGGC GCAGTGGTTGGTGTGCAGCCGTTCGGCGGCGAAGGGTTGTCCGGTACCGGGCCGAAAGCA GGCGGTCCGCTCTATCTCTACCGTCTGCTGGCGAATCGCCCGGAAAGTGCGCTGGCAGTG ACGCTCGCGCGTCAGGATGCAAAGTATCCGGTCGATGCGCAGTTGAAAGCCGCATTGACT CAGCCGCTAAATGCACTGCGGGAATGGGCAGCAAATCGTCCAGAATTGCAGGCGTTATGT ACGCAATATGGCGAGCTGGCGCAGGCAGGAACACAACGATTGCTGCCGGGGCCGACGGGT GAACGCAACACCTGGACGCTGCTGCCGCGTGAGCGCGTGTTGTGTATTGCCGATGATGAG CAGGATGCGCTGACTCAGCTCGCCGCCGTGCTGGCGGTGGGCAGCCAGGTACTGTGGCCG GATGACGCGCTGCATCGTCAGTTAGTGAAGGCATTGCCATCGGCAGTCAGCGAACGTATT CAACTGGCGAAAGCGGAAAATATAACCGCTCAACCGTTTGATGCGGTGATCTTCCACGGT GATTCGGATCAGCTTCGCGCATTGTGTGAAGCAGTTGCCGCGCGGGATGGCACAATTGTT TCGGTGCAGGGTTTTGCCCGTGGCGAAAGCAATATCCTTCTGGAACGGCTGTATATCGAG CGTTCGCTGAGTGTGAATACCGCTGCCGCTGGCGGTAACGCCAGCTTAATGACTATAGGT TAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P09546 UniProtKB Entry Name PUTA_ECOLI GenBank Protein ID 468875 GenBank Gene ID U05212 - General References
- Ling M, Allen SW, Wood JM: Sequence analysis identifies the proline dehydrogenase and delta 1-pyrroline-5-carboxylate dehydrogenase domains of the multifunctional Escherichia coli PutA protein. J Mol Biol. 1994 Nov 11;243(5):950-6. [Article]
- Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Nakao T, Yamato I, Anraku Y: Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12. Mol Gen Genet. 1987 Dec;210(2):364-8. [Article]
- Lee YH, Nadaraia S, Gu D, Becker DF, Tanner JJ: Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein. Nat Struct Biol. 2003 Feb;10(2):109-14. [Article]