Soluble lytic murein transglycosylase
Details
- Name
- Soluble lytic murein transglycosylase
- Synonyms
- 4.2.2.n1
- Exomuramidase
- Peptidoglycan lytic exotransglycosylase
- Slt70
- sltY
- Gene Name
- slt
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0003995|Soluble lytic murein transglycosylase MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAWDNRQMDVVEQMMPGLK DYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQSRFVNELARREDWRGLLA FSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGKSQPNACDKLFSVWRASGKQD PLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAIISLANNPNTVLTFARTTGATDF TRQMAAVAFASVARQDAENARLMIPSLAQAQQLNEDQIQELRDIVAWRLMGNDVTDEQAK WRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTWLARLPMEAKEKDEWRYWQADLLLERG REAEAKEILHQLMQQRGFYPMVAAQRIGEEYELKIDKAPQNVDSALTQGPEMARVRELMY WNLDNTARSEWANLVKSKSKTEQAQLARYAFNNQWWDLSVQATIAGKLWDHLEERFPLAY NDLFKRYTSGKEIPQSYAMAIARQESAWNPKVKSPVGASGLMQIMPGTATHTVKMFSIPG YSSPGQLLDPETNINIGTSYLQYVYQQFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAV AFVESIPFSETRGYVKNVLAYDAYYRYFMGDKPTLMSATEWGRRY
- Number of residues
- 645
- Molecular Weight
- 73352.56
- Theoretical pI
- 9.06
- GO Classification
- Functionscarbon-oxygen lyase activity, acting on polysaccharides / hydrolase activity, hydrolyzing O-glycosyl compounds / lytic transglycosylase activityProcessescell wall organization / peptidoglycan catabolic processComponentsmembrane / outer membrane-bounded periplasmic space
- General Function
- Lytic transglycosylase activity
- Specific Function
- Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Periplasm
- Gene sequence
>lcl|BSEQ0011456|Soluble lytic murein transglycosylase (slt) GTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACG GTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATC AAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAG GATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACG CTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGG AACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAG AGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGAT CCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTA ACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCA CTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTT ACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCA CGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAG CTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAA TGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGA ATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATG GAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGA CGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCG ATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAG AATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTAC TGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAA ACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTT CAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTAC AACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCG ATTGCTCGTCAGGAGAGCGCCTGGAATCCGAAAGTGAAATCACCGGTAGGGGCCAGCGGC TTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGT TATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTAC CTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAAC GCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTG GCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCT TATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAA TGGGGACGTCGTTACTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0AGC3 UniProtKB Entry Name SLT_ECOLI GenBank Protein ID 147836 GenBank Gene ID M69185 - General References
- Engel H, Kazemier B, Keck W: Murein-metabolizing enzymes from Escherichia coli: sequence analysis and controlled overexpression of the slt gene, which encodes the soluble lytic transglycosylase. J Bacteriol. 1991 Nov;173(21):6773-82. [Article]
- Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. Nucleic Acids Res. 1995 Jun 25;23(12):2105-19. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Singleton CK, Roeder WD, Bogosian G, Somerville RL, Weith HL: DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor. Nucleic Acids Res. 1980 Apr 11;8(7):1551-60. [Article]
- Gunsalus RP, Yanofsky C: Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21. [Article]
- Betzner AS, Keck W: Molecular cloning, overexpression and mapping of the slt gene encoding the soluble lytic transglycosylase of Escherichia coli. Mol Gen Genet. 1989 Nov;219(3):489-91. [Article]
- VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
- Rozeboom HJ, Dijkstra BW, Engel H, Keck W: Crystallization of the soluble lytic transglycosylase from Escherichia coli K12. J Mol Biol. 1990 Apr 20;212(4):557-9. [Article]
- Thunnissen AM, Dijkstra AJ, Kalk KH, Rozeboom HJ, Engel H, Keck W, Dijkstra BW: Doughnut-shaped structure of a bacterial muramidase revealed by X-ray crystallography. Nature. 1994 Feb 24;367(6465):750-3. [Article]
- Thunnissen AM, Rozeboom HJ, Kalk KH, Dijkstra BW: Structure of the 70-kDa soluble lytic transglycosylase complexed with bulgecin A. Implications for the enzymatic mechanism. Biochemistry. 1995 Oct 3;34(39):12729-37. [Article]
- van Asselt EJ, Thunnissen AM, Dijkstra BW: High resolution crystal structures of the Escherichia coli lytic transglycosylase Slt70 and its complex with a peptidoglycan fragment. J Mol Biol. 1999 Aug 27;291(4):877-98. [Article]