Thymidylate synthase 1
Details
- Name
- Thymidylate synthase 1
- Synonyms
- 2.1.1.45
- Thymidylate synthase A
- TS 1
- TS A
- TSase A
- Gene Name
- thyA1
- Organism
- Bacillus subtilis (strain 168)
- Amino acid sequence
>lcl|BSEQ0013322|Thymidylate synthase 1 MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFDNSEVPILTT KKVAWKTAIKELLWIWQLKSNDVNDLNMMGVHIWDQWKQEDGTIGHAYGFQLGKKNRSLN GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRA RSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQF EAPELWINPEVKDFYDFTIDDFKLINYKHGDKLLFEVAV
- Number of residues
- 279
- Molecular Weight
- 32807.07
- Theoretical pI
- 5.79
- GO Classification
- Functionsthymidylate synthase activityProcessesdTMP biosynthetic process / dTTP biosynthetic processComponentscytoplasm
- General Function
- Thymidylate synthase activity
- Specific Function
- Provides the sole de novo source of dTMP for DNA biosynthesis.
- Pfam Domain Function
- Thymidylat_synt (PF00303)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0013323|Thymidylate synthase 1 (thyA1) ATGACGCAATTCGATAAACAATACAATTCAATTATAAAGGATATTATCAATAATGGAATC TCAGACGAAGAGTTTGATGTAAGAACCAAGTGGGACTCAGATGGAACACCGGCACATACT CTAAGTGTAATCAGTAAGCAAATGAGATTCGACAACTCAGAGGTTCCGATTTTAACGACA AAAAAGGTTGCCTGGAAAACAGCCATTAAAGAGTTGCTCTGGATTTGGCAGCTGAAATCT AATGATGTTAATGATTTAAACATGATGGGCGTCCATATTTGGGATCAGTGGAAACAAGAA GACGGAACCATCGGACATGCATATGGATTTCAGCTGGGGAAGAAAAACAGAAGTCTAAAT GGAGAAAAAGTGGATCAGGTAGACTATCTTCTTCATCAATTGAAGAACAATCCATCTTCA CGCAGACACATTACAATGCTGTGGAATCCTGATGAATTAGACGCAATGGCCTTAACGCCA TGTGTATACGAGACACAATGGTACGTTAAACATGGGAAACTCCACCTTGAGGTAAGAGCA CGGAGCAATGATATGGCATTGGGAAATCCATTCAATGTATTCCAGTATAATGTGTTGCAG CGCATGATTGCTCAAGTGACTGGTTATGAGCTTGGTGAATATATCTTTAACATTGGGGAT TGCCATGTGTACACACGTCATATAGACAATTTGAAAATTCAAATGGAAAGAGAACAGTTT GAAGCACCTGAACTATGGATCAATCCTGAAGTGAAAGATTTTTATGACTTTACCATTGAT GATTTCAAGTTAATCAACTATAAACATGGGGACAAGCTTTTATTTGAGGTAGCGGTTTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0CI79 UniProtKB Entry Name TYSY1_BACSU GenBank Protein ID 607048 GenBank Gene ID X78560 - General References
- Tam NH, Borriss R: The thyA gene from Bacillus subtilis exhibits similarity with the phage phi 3T thymidylate synthase gene. Microbiology. 1995 Feb;141 ( Pt 2):291-7. [Article]
- Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
- Tam NH, Borriss R: Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families. Mol Gen Genet. 1998 May;258(4):427-30. [Article]
- Stout TJ, Schellenberger U, Santi DV, Stroud RM: Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis. Biochemistry. 1998 Oct 20;37(42):14736-47. [Article]
- Fox KM, Maley F, Garibian A, Changchien LM, Van Roey P: Crystal structure of thymidylate synthase A from Bacillus subtilis. Protein Sci. 1999 Mar;8(3):538-44. [Article]