Neutrophil cytosol factor 1

Details

Name
Neutrophil cytosol factor 1
Synonyms
  • 47 kDa autosomal chronic granulomatous disease protein
  • 47 kDa neutrophil oxidase factor
  • NCF-1
  • NCF-47K
  • Neutrophil NADPH oxidase factor 1
  • Nox organizer 2
  • Nox-organizing protein 2
  • NOXO2
  • p47-phox
  • SH3 and PX domain-containing protein 1A
  • SH3PXD1A
Gene Name
NCF1
Organism
Humans
Amino acid sequence
>lcl|BSEQ0008679|Neutrophil cytosol factor 1
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPI
EAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCGTLMSLPTKISRCPHLLDFFKV
RPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTG
DVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAV
EGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPP
RRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSK
PQPAVPPRPSADLILNRCSESTKRKLASAV
Number of residues
390
Molecular Weight
44651.445
Theoretical pI
Not Available
GO Classification
Functions
electron carrier activity / phosphatidylinositol binding / phosphatidylinositol-3,4-bisphosphate binding / SH3 domain binding / superoxide-generating NADPH oxidase activity
Processes
antigen processing and presentation of exogenous peptide antigen via MHC class I / antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent / antigen processing and presentation of peptide antigen via MHC class I / cell proliferation / cellular defense response / hydrogen peroxide biosynthetic process / inflammatory response / innate immune response / leukotriene metabolic process / negative regulation of smooth muscle contraction / neutrophil mediated killing of fungus / neutrophil mediated killing of gram-positive bacterium / oxidation-reduction process / phagosome maturation / protein targeting to membrane / respiratory burst / respiratory burst involved in defense response / response to yeast / small GTPase mediated signal transduction / superoxide anion generation / superoxide metabolic process / vascular endothelial growth factor receptor signaling pathway
Components
cytosol / extrinsic component of membrane / NADPH oxidase complex / phagolysosome
General Function
Superoxide-generating nadph oxidase activity
Specific Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0013349|Neutrophil cytosol factor 1 (NCF1)
ATGGGGGACACCTTCATCCGTCACATCGCCCTGCTGGGCTTTGAGAAGCGCTTCGTACCC
AGCCAGCACTATGTGTACATGTTCCTGGTGAAATGGCAGGACCTGTCGGAGAAGGTGGTC
TACCGGCGCTTCACCGAGATCTACGAGTTCCATAAAACCTTAAAAGAAATGTTCCCTATT
GAGGCAGGGGCGATCAATCCAGAGAACAGGATCATCCCCCACCTCCCAGCTCCCAAGTGG
TTTGACGGGCAGCGGGCCGCCGAGAACCGCCAGGGCACACTTACCGAGTACTGCAGCACG
CTCATGAGCCTGCCCACCAAGATCTCCCGCTGTCCCCACCTCCTCGACTTCTTCAAGGTG
CGCCCTGATGACCTCAAGCTCCCCACGGACAACCAGACAAAAAAGCCAGAGACATACTTG
ATGCCCAAAGATGGCAAGAGTACCGCGACAGACATCACCGGCCCCATCATCCTGCAGACG
TACCGCGCCATTGCCAACTACGAGAAGACCTCGGGCTCCGAGATGGCTCTGTCCACGGGG
GACGTGGTGGAGGTCGTAGAGAAGAGCGAGAGCGGTTGGTGGTTCTGTCAGATGAAAGCA
AAGCGAGGCTGGATCCCAGCGTCCTTCCTCGAGCCCCTGGACAGTCCTGACGAGACGGAA
GACCCTGAGCCCAACTATGCAGGTGAGCCATACGTCGCCATCAAGGCCTACACTGCTGTG
GAGGGGGACGAGGTGTCCCTGCTCGAGGGTGAAGCTGTTGAGGTCATTCACAAGCTCCTG
GACGGCTGGTGGGTCATCAGGAAAGACGACGTCACAGGCTACTTCCCGTCCATGTACCTG
CAAAAGTCAGGGCAAGACGTGTCCCAGGCCCAACGCCAGATCAAGCGGGGGGCGCCGCCC
CGCAGGTCGTCCATCCGCAACGCGCACAGCATCCACCAGCGGTCGCGGAAGCGCCTCAGC
CAGGACGCCTATCGCCGCAACAGCGTCCGTTTTCTGCAGCAGCGACGCCGCCAGGCGCGG
CCGGGACCGCAGAGCCCCGGGAGCCCGCTCGAGGAGGAGCGGCAGACGCAGCGCTCTAAA
CCGCAGCCGGCGGTGCCCCCGCGGCCGAGCGCCGACCTCATCCTGAACCGCTGCAGCGAG
AGCACCAAGCGGAAGCTGGCGTCTGCCGTCTGA
Chromosome Location
7
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP14598
UniProtKB Entry NameNCF1_HUMAN
HGNC IDHGNC:7660
General References
  1. Volpp BD, Nauseef WM, Donelson JE, Moser DR, Clark RA: Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7195-9. [Article]
  2. Lomax KJ, Leto TL, Nunoi H, Gallin JI, Malech HL: Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease. Science. 1989 Jul 28;245(4916):409-12. [Article]
  3. Rodaway AR, Teahan CG, Casimir CM, Segal AW, Bentley DL: Characterization of the 47-kilodalton autosomal chronic granulomatous disease protein: tissue-specific expression and transcriptional control by retinoic acid. Mol Cell Biol. 1990 Oct;10(10):5388-96. [Article]
  4. Gorlach A, Lee PL, Roesler J, Hopkins PJ, Christensen B, Green ED, Chanock SJ, Curnutte JT: A p47-phox pseudogene carries the most common mutation causing p47-phox- deficient chronic granulomatous disease. J Clin Invest. 1997 Oct 15;100(8):1907-18. [Article]
  5. Chanock SJ, Roesler J, Zhan S, Hopkins P, Lee P, Barrett DT, Christensen BL, Curnutte JT, Gorlach A: Genomic structure of the human p47-phox (NCF1) gene. Blood Cells Mol Dis. 2000 Feb;26(1):37-46. [Article]
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  21. Kami K, Takeya R, Sumimoto H, Kohda D: Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p. EMBO J. 2002 Aug 15;21(16):4268-76. [Article]
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Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB00514DextromethorphanapprovedunknowninhibitorDetails