Uroporphyrinogen-III C-methyltransferase
Details
- Name
- Uroporphyrinogen-III C-methyltransferase
- Synonyms
- 2.1.1.107
- SUMT
- Urogen III methylase
- UROM
- Uroporphyrinogen III methylase
- Gene Name
- cobA
- Organism
- Pseudomonas denitrificans
- Amino acid sequence
>lcl|BSEQ0019298|Uroporphyrinogen-III C-methyltransferase MIDDLFAGLPALEKGSVWLVGAGPGDPGLLTLHAANALRQADVIVHDALVNEDCLKLARP GAVLEFAGKRGGKPSPKQRDISLRLVELARAGNRVLRLKGGDPFVFGRGGEEALTLVEHQ VPFRIVPGITAGIGGLAYAGIPVTHREVNHAVTFLTGHDSSGLVPDRINWQGIASGSPVI VMYMAMKHIGAITANLIAGGRSPDEPVAFVCNAATPQQAVLETTLARAEADVAAAGLEPP AIVVVGEVVRLRAALDWIGALDGRKLAADPFANRILRNPA
- Number of residues
- 280
- Molecular Weight
- 29252.515
- Theoretical pI
- 7.22
- GO Classification
- Functionsprecorrin-2 dehydrogenase activity / uroporphyrin-III C-methyltransferase activityProcessescobalamin biosynthetic process / siroheme biosynthetic process
- General Function
- Uroporphyrin-iii c-methyltransferase activity
- Specific Function
- Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.
- Pfam Domain Function
- TP_methylase (PF00590)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0004964|843 bp ATGATCGACGACCTCTTTGCCGGATTGCCGGCGCTCGAAAAAGGTTCGGTCTGGCTGGTC GGCGCCGGCCCCGGCGATCCCGGCCTGTTGACGCTGCATGCGGCCAATGCGCTGCGCCAG GCGGATGTGATCGTGCATGATGCGCTGGTCAACGAGGATTGCCTGAAGCTCGCGCGGCCG GGCGCCGTGCTGGAGTTTGCGGGCAAGCGTGGCGGCAAGCCGTCGCCGAAGCAGCGCGAC ATCTCGCTTCGCCTCGTCGAACTCGCGCGCGCCGGCAACCGGGTGCTGCGCCTCAAAGGC GGCGATCCCTTCGTCTTCGGTCGCGGTGGCGAGGAGGCGCTGACGCTGGTCGAACACCAG GTGCCGTTCCGAATCGTGCCCGGCATCACCGCCGGTATCGGCGGGCTTGCCTATGCCGGC ATTCCCGTGACCCATCGCGAGGTCAACCACGCGGTCACTTTCCTGACTGGCCATGATTCC TCCGGCCTGGTGCCGGATCGCATCAACTGGCAGGGCATCGCCAGCGGCTCGCCTGTCATC GTCATGTACATGGCGATGAAACATATCGGCGCGATCACCGCCAACCTCATTGCCGGCGGC CGCTCGCCGGACGAACCGGTCGCCTTCGTCTGCAACGCCGCGACGCCGCAGCAGGCGGTG CTGGAAACGACGCTTGCGCGTGCAGAGGCCGATGTTGCGGCGGCAGGGCTGGAGCCGCCG GCGATCGTCGTCGTCGGCGAGGTGGTGCGGCTGCGCGCAGCGCTCGACTGGATCGGCGCG CTGGACGGGCGCAAGCTTGCCGCCGACCCGTTCGCCAATCGCATTCTCAGGAACCCGGCA TGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P21631 UniProtKB Entry Name SUMT_PSEDE GenBank Gene ID M59236 - General References
- Crouzet J, Cauchois L, Blanche F, Debussche L, Thibaut D, Rouyez MC, Rigault S, Mayaux JF, Cameron B: Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase. J Bacteriol. 1990 Oct;172(10):5968-79. [Article]
- Blanche F, Debussche L, Thibaut D, Crouzet J, Cameron B: Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans. J Bacteriol. 1989 Aug;171(8):4222-31. [Article]
- Vevodova J, Graham RM, Raux E, Schubert HL, Roper DI, Brindley AA, Ian Scott A, Roessner CA, Stamford NP, Elizabeth Stroupe M, Getzoff ED, Warren MJ, Wilson KS: Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis. J Mol Biol. 2004 Nov 19;344(2):419-33. [Article]