Sialidase
Details
- Name
- Sialidase
- Synonyms
- 3.2.1.18
- N-acylneuraminate glycohydrolase
- NANase
- Neuraminidase
- STNA
- Gene Name
- nanH
- Organism
- Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
- Amino acid sequence
>lcl|BSEQ0003217|Sialidase MTVEKSVVFKAEGEHFTDQKGNTIVGSGSGGTTKYFRIPAMCTTSKGTIVVFADARHNTA SDQSFIDTAAARSTDGGKTWNKKIAIYNDRVNSKLSRVMDPTCIVANIQGRETILVMVGK WNNNDKTWGAYRDKAPDTDWDLVLYKSTDDGVTFSKVETNIHDIVTKNGTISAMLGGVGS GLQLNDGKLVFPVQMVRTKNITTVLNTSFIYSTDGITWSLPSGYCEGFGSENNIIEFNAS LVNNIRNSGLRRSFETKDFGKTWTEFPPMDKKVDNRNHGVQGSTITIPSGNKLVAAHSSA QNKNNDYTRSDISLYAHNLYSGEVKLIDDFYPKVGNASGAGYSCLSYRKNVDKETLYVVY EANGSIEFQDLSRHLPVIKSYN
- Number of residues
- 382
- Molecular Weight
- 42072.835
- Theoretical pI
- 8.53
- GO Classification
- Functionsexo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activityProcessespathogenesis
- General Function
- Exo-alpha-(2->8)-sialidase activity
- Specific Function
- Cleaves the terminal sialic acid (N-acetyl neuraminic acid) from carbohydrate chains in glycoproteins providing free sialic acid which can be used as carbon and energy sources. Sialidases have been suggested to be pathogenic factors in microbial infections.
- Pfam Domain Function
- BNR_3 (PF13859)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0021303|Sialidase (nanH) TTGACCAGACATCTACTTAACTGCAGAATACTGTATATGCATCCACCACTGGATATGCAT ACACACCCATTCATTAAGGAAGGAAAGAGTATGACTGTAGAAAAATCCGTGGTTTTTAAA GCTGAAGGTGAGCATTTTACTGATCAAAAAGGCAACACTATTGTCGGTAGCGGCTCTGGG GGAACAACCAAATATTTTCGAATTCCAGCAATGTGTACAACCTCAAAAGGTACCATTGTT GTATTTGCTGATGCGCGCCATAACACTGCCAGCGATCAGTCCTTTATTGACACAGCGGCG GCCCGTAGCACAGATGGAGGGAAAACCTGGAATAAAAAAATTGCGATTTATAATGACCGT GTCAACAGTAAACTTTCCCGCGTCATGGATCCCACATGCATCGTGGCGAATATTCAGGGA AGAGAAACAATCTTAGTCATGGTTGGCAAATGGAATAATAATGATAAAACATGGGGCGCA TACAGAGATAAGGCTCCAGACACTGACTGGGATTTGGTTTTATATAAATCAACCGATGAT GGCGTTACCTTTTCAAAGGTGGAAACGAACATCCATGATATAGTAACTAAAAATGGAACG ATATCTGCAATGCTTGGCGGTGTTGGATCTGGTCTTCAGCTAAATGACGGTAAACTGGTT TTCCCGGTACAAATGGTTCGCACTAAAAATATCACCACAGTTCTGAATACATCATTCATA TACTCCACTGATGGAATAACATGGTCATTGCCATCAGGATACTGTGAGGGTTTTGGTTCA GAAAATAACATCATAGAATTTAATGCCTCTTTGGTTAATAACATCAGAAACTCTGGCCTC CGCCGTTCTTTTGAAACAAAAGATTTTGGAAAAACATGGACTGAGTTTCCTCCGATGGAC AAGAAGGTTGACAACAGGAATCATGGAGTACAAGGTTCAACAATTACTATTCCATCAGGC AATAAACTTGTCGCGGCTCACTCATCCGCGCAGAACAAGAATAATGACTATACCAGAAGT GATATATCTCTCTACGCTCACAATTTATACTCCGGGGAAGTAAAGTTAATTGATGATTTT TATCCGAAAGTCGGTAATGCCAGTGGGGCGGGTTACTCTTGTCTTTCATATCGTAAAAAT GTAGATAAAGAAACACTGTATGTTGTCTATGAAGCCAATGGAAGTATTGAGTTCCAGGAC CTTAGCCGTCATTTACCAGTAATAAAATCATACAATTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P29768 UniProtKB Entry Name NANH_SALTY GenBank Protein ID 531224 GenBank Gene ID M55342 - General References
- Hoyer LL, Hamilton AC, Steenbergen SM, Vimr ER: Cloning, sequencing and distribution of the Salmonella typhimurium LT2 sialidase gene, nanH, provides evidence for interspecies gene transfer. Mol Microbiol. 1992 Apr;6(7):873-84. [Article]
- McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK: Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001 Oct 25;413(6858):852-6. [Article]
- Taylor G, Vimr E, Garman E, Laver G: Purification, crystallization and preliminary crystallographic study of neuraminidase from Vibrio cholerae and Salmonella typhimurium LT2. J Mol Biol. 1992 Aug 20;226(4):1287-90. [Article]
- Crennell SJ, Garman EF, Laver WG, Vimr ER, Taylor GL: Crystal structure of a bacterial sialidase (from Salmonella typhimurium LT2) shows the same fold as an influenza virus neuraminidase. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):9852-6. [Article]
- Crennell SJ, Garman EF, Philippon C, Vasella A, Laver WG, Vimr ER, Taylor GL: The structures of Salmonella typhimurium LT2 neuraminidase and its complexes with three inhibitors at high resolution. J Mol Biol. 1996 Jun 7;259(2):264-80. [Article]