RNA 3'-terminal phosphate cyclase
Details
- Name
- RNA 3'-terminal phosphate cyclase
- Synonyms
- 6.5.1.4
- RNA cyclase
- yhgJ
- yhgK
- Gene Name
- rtcA
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0006359|RNA 3'-terminal phosphate cyclase MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAKPGLLRQHLTAVKAATE ICGATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADGPSRVEV SGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVSPVASFNTL QLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSLHEQNIHNLPRDQGPGNTVSLEV ESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASTAAVGEYLADQLVLPMALAGAGEF TVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTRVSIE
- Number of residues
- 338
- Molecular Weight
- 35902.925
- Theoretical pI
- 7.26
- GO Classification
- FunctionsATP binding / RNA binding / RNA-3'-phosphate cyclase activityProcessesRNA processingComponentscytoplasm
- General Function
- Rna-3'-phosphate cyclase activity
- Specific Function
- Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0012274|RNA 3'-terminal phosphate cyclase (rtcA) ATGAAAAGGATGATTGCGCTGGATGGCGCACAGGGCGAAGGTGGCGGGCAGATCCTGCGC TCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCAGCATTCGTGCC GGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTGACCGCGGTAAAGGCGGCGACGGAA ATTTGCGGAGCAACCGTGGAAGGCGCGGAGCTGGGGTCGCAGCGTCTGCTCTTCCGGCCC GGCACCGTGCGCGGCGGCGATTACCGCTTTGCTATCGGTAGCGCCGGAAGTTGTACGCTG GTGCTGCAAACGGTGCTGCCCGCGCTGTGGTTTGCCGATGGACCTTCGCGTGTTGAAGTG AGCGGCGGCACCGATAACCCGTCGGCGCCGCCTGCGGATTTTATCCGCCGGGTGCTGGAG CCGCTGCTGGCGAAAATAGGAATTCATCAGCAAACCACGCTGTTACGCCACGGTTTTTAC CCTGCCGGAGGCGGTGTGGTGGCAACGGAAGTCTCGCCCGTGGCATCGTTTAACACCTTG CAACTTGGCGAGCGCGGGAACATTGTGCAGATGCGTGGAGAAGTTCTATTAGCTGGTGTG CCGCGCCATGTTGCTGAGCGTGAAATCGCTACACTGGCGGGGAGTTTTTCCCTGCATGAA CAGAATATTCATAACCTGCCGCGCGACCAGGGGCCGGGTAATACCGTCTCGCTTGAAGTC GAAAGTGAAAATATCACCGAACGCTTTTTTGTCGTCGGTGAAAAGCGCGTCAGTGCCGAG GTGGTTGCGGCACAGTTGGTGAAAGAGGTGAAACGCTACCTGGCAAGCACGGCGGCGGTG GGGGAATATCTTGCTGACCAACTGGTGCTACCGATGGCGCTGGCGGGCGCGGGGGAATTT ACGGTCGCCCATCCCTCATGCCATCTGCTGACCAATATCGCGGTGGTGGAGCGTTTCTTG CCGGTGCGGTTTAGTTTGATAGAAACAGATGGCGTAACGCGGGTGAGCATTGAATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P46849 UniProtKB Entry Name RTCA_ECOLI GenBank Gene ID U00096 - General References
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G 3rd, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL: Escherichia coli K-12: a cooperatively developed annotation snapshot--2005. Nucleic Acids Res. 2006 Jan 5;34(1):1-9. Print 2006. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Cole ST, Raibaud O: The nucleotide sequence of the malT gene encoding the positive regulator of the Escherichia coli maltose regulon. Gene. 1986;42(2):201-8. [Article]
- Genschik P, Billy E, Swianiewicz M, Filipowicz W: The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea. EMBO J. 1997 May 15;16(10):2955-67. [Article]
- Genschik P, Drabikowski K, Filipowicz W: Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon. J Biol Chem. 1998 Sep 25;273(39):25516-26. [Article]
- Palm GJ, Billy E, Filipowicz W, Wlodawer A: Crystal structure of RNA 3'-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology. Structure. 2000 Jan 15;8(1):13-23. [Article]
- Tanaka N, Smith P, Shuman S: Structure of the RNA 3'-phosphate cyclase-adenylate intermediate illuminates nucleotide specificity and covalent nucleotidyl transfer. Structure. 2010 Mar 14;18(4):449-57. doi: 10.1016/j.str.2010.01.016. [Article]