Neopullulanase 2
Details
- Name
- Neopullulanase 2
- Synonyms
- 3.2.1.135
- Alpha-amylase II
- TVA II
- Gene Name
- tvaII
- Organism
- Thermoactinomyces vulgaris
- Amino acid sequence
>lcl|BSEQ0011001|Neopullulanase 2 MLLEAIFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRYASPEEELAHALAGKA GSDERFDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAERSKAGVFQYAYIHRSE VFTTPEWAKEAVIYQIFPERFANGDPSNDPPGTEQWAKDARPRHDSFYGGDLKGVIDRLP YLEELGVTALYFTPIFASPSHHKYDTADYLAIDPQFGDLPTFRRLVDEAHRRGIKIILDA VFNHAGDQFFAFRDVLQKGEQSRYKDWFFIEDFPVSKTSRTNYETFAVQVPAMPKLRTEN PEVKEYLFDVARFWMEQGIDGWRLDVANEVDHAFWREFRRLVKSLNPDALIVGEIWHDAS GWLMGDQFDSVMNYLFRESVIRFFATGEIHAERFDAELTRARMLYPEQAAQGLWNLLDSH DTERFLTSCGGNEAKFRLAVLFQMTYLGTPLIYYGDEIGMAGATDPDCRRPMIWEEKEQN RGLFEFYKELIRLRHRLASLTRGNVRSWHADKQANLYAFVRTVQDQHVGVVLNNRGEKQT VLLQVPESGGKTWLDCLTGEEVHGKQGQLKLTLRPYQGMILWNGR
- Number of residues
- 585
- Molecular Weight
- 67466.71
- Theoretical pI
- 5.89
- GO Classification
- Functionsmetal ion binding / neopullulanase activityProcessescarbohydrate metabolic process
- General Function
- Neopullulanase activity
- Specific Function
- Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0002832|1758 bp ATGCTGTTGGAAGCGATTTTTCATGAAGCGAAAGGAAGCTATGCTTACCCGATTTCGGAA ACGCAACTGCGGGTGAGATTGCGGGCGAAAAAAGGGGACGTGGTGAGATGCGAGGTGCTC TATGCGGACCGCTATGCTTCGCCGGAGGAAGAACTGGCACATGCACTTGCGGGCAAAGCA GGGTCCGATGAGCGATTTGATTATTTTGAGGCTTTACTGGAATGTTCCACCAAACGGGTG AAATACGTGTTTTTGTTGACAGGCCCACAAGGGGAGGCTGTTTATTTTGGCGAGACCGGT TTTTCCGCCGAACGGTCTAAAGCGGGGGTGTTCCAATATGCTTACATCCACCGGAGCGAA GTGTTTACGACACCGGAATGGGCAAAGGAGGCGGTGATCTACCAGATCTTTCCCGAACGT TTTGCCAATGGGGATCCCAGCAATGATCCGCCGGGAACGGAACAATGGGCCAAAGACGCC AGACCTCGGCACGACAGTTTTTACGGCGGAGATTTAAAGGGGGTGATCGACCGTTTGCCA TATTTGGAGGAGTTGGGAGTGACGGCGCTTTATTTTACTCCCATTTTTGCTTCGCCGTCC CATCACAAATATGATACAGCTGATTATTTGGCGATTGATCCGCAATTTGGGGATTTGCCC ACCTTTCGGCGTTTGGTGGATGAAGCTCACCGGCGCGGGATCAAAATCATTTTGGATGCC GTGTTTAACCATGCCGGAGATCAGTTTTTTGCGTTTCGGGATGTTCTGCAAAAGGGAGAA CAGTCGCGATATAAGGATTGGTTTTTCATCGAGGATTTTCCCGTTTCCAAGACGTCAAGA ACCAATTATGAAACCTTTGCCGTCCAAGTGCCGGCCATGCCCAAATTGCGGACGGAAAAT CCGGAAGTGAAGGAATATTTGTTTGATGTCGCGCGATTTTGGATGGAGCAGGGGATCGAC GGATGGAGACTGGATGTGGCCAATGAGGTGGATCACGCCTTTTGGCGCGAGTTTCGCCGC CTTGTCAAATCCCTCAATCCCGACGCCTTGATTGTCGGAGAGATTTGGCATGACGCTTCC GGCTGGCTGATGGGAGACCAATTTGATTCGGTGATGAATTATTTGTTTCGCGAGAGTGTG ATCCGTTTTTTTGCCACGGGGGAGATCCATGCCGAAAGATTTGACGCCGAACTTACCCGG GCCAGGATGCTTTATCCGGAACAGGCGGCACAAGGGTTATGGAACCTCCTCGACTCACAC GATACGGAACGTTTTTTGACATCGTGTGGGGGGAATGAGGCGAAATTCCGCCTGGCTGTT TTGTTTCAGATGACATACCTGGGGACGCCGCTGATTTATTACGGTGATGAGATCGGGATG GCCGGAGCGACGGACCCGGATTGCCGGCGCCCGATGATATGGGAGGAAAAAGAGCAGAAC CGCGGCTTGTTTGAGTTTTATAAGGAATTGATTCGCTTGCGCCATCGGCTGGCATCGCTC ACCAGGGGAAATGTCCGGTCTTGGCATGCCGACAAACAAGCCAATCTTTATGCTTTTGTG CGCACGGTTCAAGACCAACATGTCGGTGTGGTTCTCAATAATCGTGGCGAGAAGCAAACC GTTTTGCTACAGGTGCCGGAATCGGGCGGAAAAACTTGGCTGGATTGTTTGACCGGAGAA GAAGTGCATGGCAAACAAGGGCAACTGAAGCTGACACTTCGCCCTTACCAGGGAATGATT CTCTGGAATGGCCGGTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q08751 UniProtKB Entry Name NEPU2_THEVU GenBank Protein ID 398125 GenBank Gene ID D13178 - General References
- Tonozuka T, Ohtsuka M, Mogi S, Sakai H, Ohta T, Sakano Y: A neopullulanase-type alpha-amylase gene from Thermoactinomyces vulgaris R-47. Biosci Biotechnol Biochem. 1993 Mar;57(3):395-401. [Article]
- Kamitori S, Kondo S, Okuyama K, Yokota T, Shimura Y, Tonozuka T, Sakano Y: Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVAII) hydrolyzing cyclodextrins and pullulan at 2.6 A resolution. J Mol Biol. 1999 Apr 16;287(5):907-21. [Article]
- Kamitori S, Abe A, Ohtaki A, Kaji A, Tonozuka T, Sakano Y: Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution. J Mol Biol. 2002 Apr 26;318(2):443-53. [Article]