(S)-2-haloacid dehalogenase
Details
- Name
- (S)-2-haloacid dehalogenase
- Synonyms
- 2-haloalkanoic acid dehalogenase
- 3.8.1.2
- Halocarboxylic acid halidohydrolase
- L-2-haloacid dehalogenase
- L-DEX
- Gene Name
- Not Available
- Organism
- Pseudomonas sp. (strain YL)
- Amino acid sequence
>lcl|BSEQ0011172|(S)-2-haloacid dehalogenase MDYIKGIAFDLYGTLFDVHSVVGRCDEAFPGRGREISALWRQKQLEYTWLRSLMNRYVNF QQATEDALRFTCRHLGLDLDARTRSTLCDAYLRLAPFSEVPDSLRELKRRGLKLAILSNG SPQSIDAVVSHAGLRDGFDHLLSVDPVQVYKPDNRVYELAEQALGLDRSAILFVSSNAWD ATGARYFGFPTCWINRTGNVFEEMGQTPDWEVTSLRAVVELFETAAGKAEKG
- Number of residues
- 232
- Molecular Weight
- 26176.335
- Theoretical pI
- 5.72
- GO Classification
- Functions(S)-2-haloacid dehalogenase activity
- General Function
- (s)-2-haloacid dehalogenase activity
- Specific Function
- Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2.
- Pfam Domain Function
- HAD_2 (PF13419)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0003266|699 bp ATGGACTACATCAAGGGTATTGCCTTCGACCTTTACGGTACGCTGTTCGACGTCCATTCG GTTGTCGGCCGATGCGACGAGGCGTTCCCGGGTCGCGGGCGCGAGATCAGCGCGCTGTGG CGGCAAAAGCAGCTCGAGTACACATGGCTGCGAAGCCTGATGAATCGCTACGTTAACTTC CAACAGGCGACCGAAGACGCGTTGCGGTTTACCTGCCGGCATCTTGGGCTCGATCTGGAT GCGAGAACCCGCAGCACGCTTTGCGACGCGTACTTGCGGCTTGCACCGTTCAGCGAGGTG CCCGATTCGCTACGCGAACTTAAGCGGCGCGGTCTGAAGCTCGCCATCCTGTCGAACGGC TCGCCGCAGTCGATCGATGCCGTCGTGAGCCATGCGGGGCTGCGGGATGGATTCGACCAC CTGCTCAGCGTCGATCCGGTACAGGTCTACAAACCCGATAACCGCGTGTATGAACTCGCG GAGCAGGCGTTGGGGCTCGACCGATCGGCGATCCTGTTCGTGTCGTCGAACGCGTGGGAC GCAACGGGGGCGCGCTACTTCGGCTTCCCGACATGCTGGATCAATCGGACCGGCAACGTC TTTGAAGAGATGGGGCAGACACCCGACTGGGAGGTAACCAGCCTCCGCGCCGTGGTGGAG CTGTTCGAGACGGCCGCCGGGAAGGCAGAAAAGGGCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q53464 UniProtKB Entry Name HAD_PSEUY GenBank Protein ID 691747 GenBank Gene ID S74078 - General References
- Nardi-Dei V, Kurihara T, Okamura T, Liu JQ, Koshikawa H, Ozaki H, Terashima Y, Esaki N, Soda K: Comparative studies of genes encoding thermostable L-2-halo acid dehalogenase from Pseudomonas sp. strain YL, other dehalogenases, and two related hypothetical proteins from Escherichia coli. Appl Environ Microbiol. 1994 Sep;60(9):3375-80. [Article]
- Kurihara T, Liu JQ, Nardi-Dei V, Koshikawa H, Esaki N, Soda K: Comprehensive site-directed mutagenesis of L-2-halo acid dehalogenase to probe catalytic amino acid residues. J Biochem. 1995 Jun;117(6):1317-22. [Article]
- Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K: Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold. J Biol Chem. 1996 Aug 23;271(34):20322-30. [Article]
- Li YF, Hata Y, Fujii T, Hisano T, Nishihara M, Kurihara T, Esaki N: Crystal structures of reaction intermediates of L-2-haloacid dehalogenase and implications for the reaction mechanism. J Biol Chem. 1998 Jun 12;273(24):15035-44. [Article]