Holliday junction ATP-dependent DNA helicase RuvB
Details
- Name
- Holliday junction ATP-dependent DNA helicase RuvB
- Synonyms
- 3.6.4.12
- Gene Name
- ruvB
- Organism
- Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
- Amino acid sequence
>lcl|BSEQ0011070|Holliday junction ATP-dependent DNA helicase RuvB MSEFLTPERTVYDSGVQFLRPKSLDEFIGQENVKKKLSLALEAAKMRGEVLDHVLLAGPP GLGKTTLAHIIASELQTNIHVTSGPVLVKQGDMAAILTSLERGDVLFIDEIHRLNKAVEE LLYSAIEDFQIDIMIGKGPSAKSIRIDIQPFTLVGATTRSGLLSSPLRSRFGIILELDFY TVKELKEIIKRAASLMDVEIEDAAAEMIAKRSRGTPRIAIRLTKRVRDMLTVVKADRINT DIVLKTMEVLNIDDEGLDEFDRKILKTIIEIYRGGPVGLNALAASLGVEADTLSEVYEPY LLQAGFLARTPRGRIVTEKAYKHLKYEVPENRLF
- Number of residues
- 334
- Molecular Weight
- 37155.775
- Theoretical pI
- 6.39
- GO Classification
- FunctionsATP binding / DNA binding / four-way junction helicase activityProcessesDNA recombination / DNA repair / SOS response
- General Function
- Four-way junction helicase activity
- Specific Function
- The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0011071|Holliday junction ATP-dependent DNA helicase RuvB (ruvB) GTGAGTGAATTTCTCACACCTGAAAGGACCGTTTACGACTCTGGTGTACAGTTTCTAAGG CCCAAAAGCCTCGATGAATTCATTGGTCAGGAAAACGTGAAAAAGAAACTCTCCCTCGCT CTCGAAGCCGCGAAGATGAGGGGAGAAGTGCTCGATCATGTCCTCCTCGCAGGACCACCG GGACTCGGAAAGACGACCCTTGCACACATAATCGCCAGCGAACTCCAGACGAACATCCAC GTTACGAGCGGACCGGTTCTTGTGAAACAGGGAGATATGGCCGCTATCCTCACAAGTCTG GAACGGGGAGACGTTCTTTTCATAGACGAAATACACCGATTGAACAAGGCAGTGGAAGAG CTTCTTTACTCTGCCATCGAAGACTTCCAGATAGACATCATGATCGGAAAGGGCCCGAGT GCAAAGTCCATTAGGATAGACATCCAGCCTTTTACGCTCGTTGGAGCCACGACGAGAAGT GGTCTTTTGAGTTCTCCTCTCAGAAGCAGGTTTGGTATCATCCTCGAACTGGACTTCTAC ACTGTGAAAGAACTGAAGGAAATCATAAAAAGAGCGGCCAGCTTGATGGACGTTGAGATA GAAGACGCAGCAGCAGAGATGATCGCGAAAAGATCGAGAGGCACACCGAGGATCGCTATA AGACTCACGAAGAGAGTGAGGGACATGCTCACGGTGGTAAAGGCAGACAGAATCAATACC GATATCGTTTTGAAGACCATGGAAGTTCTGAACATAGACGACGAGGGACTTGATGAGTTC GACAGGAAGATCCTGAAGACGATCATAGAGATTTACAGGGGAGGCCCCGTCGGATTGAAC GCCCTCGCCGCTTCACTCGGTGTAGAAGCGGACACCCTGAGCGAAGTTTATGAACCTTAC CTCCTCCAGGCAGGATTCCTCGCCAGAACTCCCAGAGGAAGGATCGTCACTGAAAAGGCT TACAAACACCTGAAGTACGAAGTCCCGGAAAACCGTCTTTTCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q56313 UniProtKB Entry Name RUVB_THEMA GenBank Protein ID 1063668 GenBank Gene ID U38840 - General References
- Tong J, Wetmur JG: Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria. J Bacteriol. 1996 May;178(9):2695-700. [Article]
- Nelson KE, Clayton RA, Gill SR, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Nelson WC, Ketchum KA, McDonald L, Utterback TR, Malek JA, Linher KD, Garrett MM, Stewart AM, Cotton MD, Pratt MS, Phillips CA, Richardson D, Heidelberg J, Sutton GG, Fleischmann RD, Eisen JA, White O, Salzberg SL, Smith HO, Venter JC, Fraser CM: Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima. Nature. 1999 May 27;399(6734):323-9. [Article]