2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Details

Name
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Synonyms
  • 4.6.1.12
  • MECDP-synthase
Gene Name
ispF
Organism
Shewanella oneidensis (strain MR-1)
Amino acid sequence
>lcl|BSEQ0012848|2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
MKIRIGHGFDVHKFGEPRPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIG
KHFPDTDAAYKGADSRVLLRHCYALAKAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAA
DLNADVADINVKATTTEKLGFTGRKEGIAVEAVVLLSRQ
Number of residues
159
Molecular Weight
16995.47
Theoretical pI
6.39
GO Classification
Functions
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity / metal ion binding
Processes
isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway / terpenoid biosynthetic process
General Function
Metal ion binding
Specific Function
Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (By similarity).
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0012849|2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
ATGAAAATCCGAATAGGCCATGGTTTTGATGTCCATAAATTTGGTGAACCACGCCCGTTA
ATTTTATGTGGCGTCGAAGTACCCTACGAAACTGGCTTGGTGGCCCACTCCGATGGTGAC
GTGGTGTTACATGCCATATCCGACGCCATTTTAGGCGCGATGGCGCTGGGTGACATTGGC
AAACATTTCCCCGATACCGATGCAGCCTATAAGGGCGCAGATAGTCGAGTCTTGCTTCGC
CACTGCTATGCGTTAGCAAAGGCGAAGGGATTTGAGCTGGGCAATCTGGATGTCACTATC
ATTGCGCAGGCGCCTAAAATGGCGCCGCATATCGAGGATATGCGCCAAGTGCTAGCAGCC
GATCTCAATGCTGATGTTGCTGACATTAATGTGAAGGCCACTACCACTGAGAAACTCGGG
TTTACGGGCCGAAAAGAAGGCATTGCAGTCGAAGCTGTCGTATTACTCAGCCGCCAATAG
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ8EBR3
UniProtKB Entry NameISPF_SHEON
GenBank Protein ID24345351
GenBank Gene IDAE014299
General References
  1. Heidelberg JF, Paulsen IT, Nelson KE, Gaidos EJ, Nelson WC, Read TD, Eisen JA, Seshadri R, Ward N, Methe B, Clayton RA, Meyer T, Tsapin A, Scott J, Beanan M, Brinkac L, Daugherty S, DeBoy RT, Dodson RJ, Durkin AS, Haft DH, Kolonay JF, Madupu R, Peterson JD, Umayam LA, White O, Wolf AM, Vamathevan J, Weidman J, Impraim M, Lee K, Berry K, Lee C, Mueller J, Khouri H, Gill J, Utterback TR, McDonald LA, Feldblyum TV, Smith HO, Venter JC, Nealson KH, Fraser CM: Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis. Nat Biotechnol. 2002 Nov;20(11):1118-23. Epub 2002 Oct 7. [Article]
  2. Ni S, Robinson H, Marsing GC, Bussiere DE, Kennedy MA: Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase from Shewanella oneidensis at 1.6 A: identification of farnesyl pyrophosphate trapped in a hydrophobic cavity. Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1949-57. Epub 2004 Oct 20. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB07780Farnesyl diphosphateexperimentalunknownDetails