Serine/threonine-protein kinase RIO1

Details

Name

Serine/threonine-protein kinase RIO1

Synonyms

• 2.7.11.1
• RIO kinase 1

Gene Name

RIOK1

Organism

Humans

Amino acid sequence

>lcl|BSEQ0051934|Serine/threonine-protein kinase RIO1
MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEE
EGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKI
NLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIIT
EINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPR
KMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKA
RELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKD
CANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHV
DEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQ
KVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKE
AQREKRKNKIPKHVKKRKEKTAKTKKGK

Number of residues

568

Molecular Weight

65582.485

Theoretical pI

Not Available

GO Classification

Functions

ATP binding / hydrolase activity / metal ion binding / protein serine/threonine kinase activity

Processes

maturation of SSU-rRNA / positive regulation of rRNA processing / ribosomal small subunit biogenesis / rRNA processing

Components

cytosol / nucleoplasm / preribosome, small subunit precursor

General Function

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity).

Specific Function

Atp binding

Pfam Domain Function

Not Available

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0051935|Serine/threonine-protein kinase RIO1 (RIOK1)
ATGGACTACCGGCGGCTTCTCATGAGCCGGGTGGTCCCCGGGCAATTCGACGACGCGGAC
TCCTCTGACAGTGAAAACAGAGACTTGAAGACAGTCAAAGAGAAGGATGACATTCTGTTT
GAAGACCTTCAAGACAATGTGAATGAGAATGGTGAAGGTGAAATAGAAGATGAGGAGGAG
GAGGGTTATGACGATGATGATGATGACTGGGACTGGGATGAAGGAGTTGGAAAACTCGCC
AAGGGTTATGTCTGGAATGGAGGAAGCAACCCACAGGCAAATCGACAGACCTCCGACAGC
AGTTCAGCCAAAATGTCTACTCCAGCAGACAAGGTCTTACGGAAATTTGAGAATAAAATT
AATTTAGATAAGCTAAATGTTACTGATTCCGTCATAAATAAAGTCACCGAAAAGTCTAGA
CAAAAGGAAGCAGATATGTATCGCATCAAAGATAAGGCAGACAGAGCAACTGTAGAACAG
GTGTTGGATCCCAGAACAAGAATGATTTTATTCAAGATGTTGACTAGAGGAATCATAACA
GAGATAAATGGCTGCATTAGCACAGGAAAAGAAGCTAATGTATACCATGCTAGCACAGCA
AATGGAGAGAGCAGAGCAATCAAAATTTATAAAACTTCTATTTTGGTGTTCAAAGATCGG
GATAAATATGTAAGTGGAGAATTCAGATTTCGTCATGGCTATTGTAAAGGAAACCCTAGG
AAAATGGTGAAAACTTGGGCAGAAAAAGAAATGAGGAACTTAATCAGGCTAAACACAGCA
GAGATACCATGTCCAGAACCAATAATGCTAAGAAGTCATGTTCTTGTCATGAGTTTCATC
GGTAAAGATGACATGCCTGCACCACTCTTGAAAAATGTCCAGTTATCAGAATCCAAGGCT
CGGGAGTTGTACCTGCAGGTCATTCAGTACATGAGAAGAATGTATCAGGATGCCAGACTT
GTCCATGCAGATCTCAGTGAATTTAACATGCTGTACCACGGTGGAGGCGTGTATATCATT
GACGTGTCTCAGTCCGTGGAGCACGACCACCCACATGCCTTGGAGTTCTTGAGAAAGGAT
TGCGCCAACGTCAATGATTTCTTTATGAGGCACAGTGTTGCTGTCATGACTGTGCGGGAG
CTCTTTGAATTTGTCACAGATCCATCCATTACACATGAGAACATGGATGCTTATCTCTCA
AAGGCCATGGAAATAGCATCTCAAAGGACCAAGGAAGAACGGTCTAGCCAAGATCATGTG
GATGAAGAGGTGTTTAAGCGAGCATATATTCCTAGAACCTTGAATGAAGTGAAAAATTAT
GAGAGGGATATGGACATAATTATGAAATTGAAGGAAGAGGACATGGCCATGAATGCCCAA
CAAGATAATATTCTATACCAGACTGTTACAGGATTGAAGAAAGATTTGTCAGGAGTTCAG
AAGGTCCCTGCACTCCTAGAAAATCAAGTGGAGGAAAGGACTTGTTCTGATTCAGAAGAT
ATTGGAAGCTCTGAGTGCTCTGACACAGACTCTGAAGAGCAGGGAGACCATGCCCGCCCC
AAGAAACACACCACGGACCCTGACATTGATAAAAAAGAAAGAAAAAAGATGGTCAAGGAA
GCCCAGAGAGAGAAAAGAAAAAACAAAATTCCTAAACATGTGAAAAAAAGAAAGGAGAAG
ACAGCCAAGACGAAAAAAGGCAAATAG

Chromosome Location

6

Locus

6p24.3

External Identifiers

Resource	Link
UniProtKB ID	Q9BRS2
UniProtKB Entry Name	RIOK1_HUMAN
HGNC ID	HGNC:18656

General References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [Article]
2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
3. Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007 Oct 31;8:399. [Article]
4. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [Article]
5. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [Article]
6. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [Article]
7. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. [Article]
8. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
9. Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. [Article]
10. Widmann B, Wandrey F, Badertscher L, Wyler E, Pfannstiel J, Zemp I, Kutay U: The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits. Mol Biol Cell. 2012 Jan;23(1):22-35. doi: 10.1091/mbc.E11-07-0639. Epub 2011 Nov 9. [Article]
11. Zhou H, Di Palma S, Preisinger C, Peng M, Polat AN, Heck AJ, Mohammed S: Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res. 2013 Jan 4;12(1):260-71. doi: 10.1021/pr300630k. Epub 2012 Dec 18. [Article]
12. Ferreira-Cerca S, Kiburu I, Thomson E, LaRonde N, Hurt E: Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes. Nucleic Acids Res. 2014 Jul;42(13):8635-47. doi: 10.1093/nar/gku542. Epub 2014 Jun 19. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB12010	Fostamatinib	approved, investigational	unknown	inhibitor	Details