Reducing end xylose-releasing exo-oligoxylanase
Details
- Name
- Reducing end xylose-releasing exo-oligoxylanase
- Synonyms
- 3.2.1.156
- Rex
- Gene Name
- Not Available
- Organism
- Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
- Amino acid sequence
>lcl|BSEQ0016975|Reducing end xylose-releasing exo-oligoxylanase MKKTTEGAFYTREYRNLFKEFGYSEAEIQERVKDTWEQLFGDNPETKIYYEVGDDLGYLL DTGNLDVRTEGMSYGMMMAVQMDRKDIFDRIWNWTMKNMYMTEGVHAGYFAWSCQPDGTK NSWGPAPDGEEYFALALFFASHRWGDGDEQPFNYSEQARKLLHTCVHNGEGGPGHPMWNR DNKLIKFIPEVEFSDPSYHLPHFYELFSLWANEEDRVFWKEAAEASREYLKIACHPETGL APEYAYYDGTPNDEKGYGHFFSDSYRVAANIGLDAEWFGGSEWSAEEINKIQAFFADKEP EDYRRYKIDGEPFEEKSLHPVGLIATNAMGSLASVDGPYAKANVDLFWNTPVRTGNRRYY DNCLYLFAMLALSGNFKIWFPEGQEEEH
- Number of residues
- 388
- Molecular Weight
- 45009.595
- Theoretical pI
- 4.45
- GO Classification
- Functionsoligosaccharide reducing-end xylanase activityProcessesxylan catabolic process
- General Function
- Oligosaccharide reducing-end xylanase activity
- Specific Function
- Hydrolyzes xylooligosaccharides with a degree of polymerization of greater than or equal to 3, releasing xylose from the reducing end. Only hydrolyzes the beta anomers of xylooligosaccharides, with inversion of anomeric configuration. Hydrolyzes the glucose and xylose-based trisaccharides where xylose is located at the -1 subsite, GXX, XXG and GXG. Does not hydrolyze xylan, chitosan, lichenan, curdlan or carboxymethylcellulose.
- Pfam Domain Function
- Glyco_hydro_8 (PF01270)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0016976|Reducing end xylose-releasing exo-oligoxylanase ATGAAGAAAACGACAGAAGGTGCATTTTATACGAGGGAGTACCGAAATCTTTTCAAGGAA TTCGGGTATTCGGAAGCGGAAATCCAAGAACGAGTTAAGGACACATGGGAACAGCTTTTT GGCGATAACCCAGAAACGAAAATTTATTATGAAGTGGGCGATGATCTAGGTTATCTCCTC GATACGGGAAACCTCGATGTGCGGACTGAAGGAATGTCCTACGGAATGATGATGGCTGTT CAAATGGACAGAAAGGATATTTTCGACCGGATCTGGAATTGGACGATGAAGAATATGTAT ATGACGGAAGGCGTGCACGCGGGCTACTTCGCTTGGTCATGCCAGCCTGATGGCACTAAA AACTCCTGGGGCCCAGCTCCGGACGGAGAGGAATATTTTGCCCTTGCATTATTTTTTGCT TCGCACCGCTGGGGCGATGGTGATGAACAGCCGTTTAATTACAGCGAACAAGCTCGTAAG CTACTTCATACTTGCGTGCACAATGGTGAAGGTGGTCCTGGCCATCCAATGTGGAACCGG GATAATAAGCTCATTAAATTCATACCTGAAGTGGAGTTCAGTGATCCCTCTTATCATCTC CCCCATTTTTATGAACTGTTCAGTTTATGGGCAAACGAAGAGGACCGCGTCTTCTGGAAA GAGGCTGCTGAAGCGAGCCGGGAGTATTTGAAAATAGCTTGCCATCCTGAAACAGGACTT GCGCCTGAGTATGCTTACTATGATGGAACGCCAAATGATGAAAAGGGATACGGCCACTTT TTTAGCGATTCTTATCGTGTGGCTGCTAATATTGGACTTGACGCTGAATGGTTCGGAGGA AGCGAGTGGTCGGCAGAAGAGATAAATAAGATCCAGGCGTTTTTTGCAGATAAGGAGCCT GAAGACTACCGCCGCTATAAAATAGACGGGGAACCGTTCGAAGAGAAATCATTGCACCCT GTCGGACTGATTGCTACTAACGCGATGGGTTCTTTAGCCTCTGTTGATGGACCATACGCT AAAGCTAATGTGGATTTGTTCTGGAACACACCGGTTCGCACCGGCAACCGCCGTTATTAC GATAATTGTTTATATTTGTTTGCGATGCTCGCACTAAGCGGCAATTTTAAAATTTGGTTT CCTGAGGGCCAAGAAGAGGAACACTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9KB30 UniProtKB Entry Name REOX_BACHD GenBank Gene ID BA000004 - General References
- Takami H, Nakasone K, Takaki Y, Maeno G, Sasaki R, Masui N, Fuji F, Hirama C, Nakamura Y, Ogasawara N, Kuhara S, Horikoshi K: Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res. 2000 Nov 1;28(21):4317-31. [Article]
- Honda Y, Kitaoka M: A family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase. J Biol Chem. 2004 Dec 31;279(53):55097-103. Epub 2004 Oct 18. [Article]
- Honda Y, Kitaoka M: The first glycosynthase derived from an inverting glycoside hydrolase. J Biol Chem. 2006 Jan 20;281(3):1426-31. Epub 2005 Nov 21. [Article]
- Honda Y, Fushinobu S, Hidaka M, Wakagi T, Shoun H, Taniguchi H, Kitaoka M: Alternative strategy for converting an inverting glycoside hydrolase into a glycosynthase. Glycobiology. 2008 Apr;18(4):325-30. doi: 10.1093/glycob/cwn011. Epub 2008 Feb 9. [Article]
- Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M: Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125. J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. [Article]