N(2)-(2-carboxyethyl)arginine synthase
Details
- Name
- N(2)-(2-carboxyethyl)arginine synthase
- Synonyms
- 2.5.1.66
- CEA synthetase
- Gene Name
- ceaS
- Organism
- Streptomyces clavuligerus
- Amino acid sequence
>lcl|BSEQ0019401|N(2)-(2-carboxyethyl)arginine synthase MSRVSTAPSGKPTAAHALLSRLRDHGVGKVFGVVGREAASILFDEVEGIDFVLTRHEFTA GVAADVLARITGRPQACWATLGPGMTNLSTGIATSVLDRSPVIALAAQSESHDIFPNDTH QCLDSVAIVAPMSKYAVELQRPHEITDLVDSAVNAAMTEPVGPSFISLPVDLLGSSEGID TTVPNPPANTPAKPVGVVADGWQKAADQAAALLAEAKHPVLVVGAAAIRSGAVPAIRALA ERLNIPVITTYIAKGVLPVGHELNYGAVTGYMDGILNFPALQTMFAPVDLVLTVGYDYAE DLRPSMWQKGIEKKTVRISPTVNPIPRVYRPDVDVVTDVLAFVEHFETATASFGAKQRHD IEPLRARIAEFLADPETYEDGMRVHQVIDSMNTVMEEAAEPGEGTIVSDIGFFRHYGVLF ARADQPFGFLTSAGCSSFGYGIPAAIGAQMARPDQPTFLIAGDGGFHSNSSDLETIARLN LPIVTVVVNNDTNGLIELYQNIGHHRSHDPAVKFGGVDFVALAEANGVDATRATNREELL AALRKGAELGRPFLIEVPVNYDFQPGGFGALSI
- Number of residues
- 573
- Molecular Weight
- 60906.59
- Theoretical pI
- 4.85
- GO Classification
- Functionsmagnesium ion binding / N2-(2-carboxyethyl)arginine synthase activity / thiamine pyrophosphate binding
- General Function
- Thiamine pyrophosphate binding
- Specific Function
- Involved in the biosynthesis of the beta-lactamase inhibitor, clavulanic acid. Catalyzes the thiamine diphosphate (ThDP) dependent condensation of D-glyceraldehyde-3-phosphate (D-G3P) with L-arginine to yield the beta-amino acid, N2-(2-carboxyethyl)arginine (CEA) via a beta-elimination resulting in the formation of an enol which undergoes a second elimination to generate the alpha,beta-unsaturated acryloyl-ThDP.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0006386|1722 bp ATGTCCCGTGTATCGACCGCCCCCAGCGGCAAGCCTACCGCCGCTCACGCCCTCCTGTCA CGGTTGCGTGATCACGGTGTGGGGAAGGTGTTTGGGGTTGTCGGCCGAGAGGCCGCGTCG ATTCTCTTCGACGAGGTCGAGGGGATCGACTTCGTTCTGACCCGCCACGAGTTCACCGCG GGTGTCGCCGCTGATGTCCTCGCGCGGATCACCGGTCGCCCCCAGGCGTGCTGGGCCACC CTGGGCCCCGGTATGACCAACCTCTCCACCGGTATCGCCACGTCCGTCCTGGACCGCTCG CCGGTCATCGCGCTCGCCGCGCAGTCGGAGTCGCACGACATCTTCCCGAACGACACCCAC CAGTGCCTGGACTCGGTGGCGATCGTCGCCCCGATGTCCAAGTACGCCGTGGAGCTCCAG CGGCCCCACGAGATCACCGACCTCGTCGACTCCGCCGTGAACGCGGCCATGACCGAGCCG GTCGGGCCCTCCTTCATCTCCCTCCCGGTGGACCTGCTCGGCTCCTCCGAGGGCATCGAC ACCACCGTCCCCAACCCGCCGGCGAACACCCCGGCGAAACCGGTCGGCGTCGTCGCCGAC GGCTGGCAGAAGGCCGCCGACCAGGCCGCCGCCCTGCTCGCCGAGGCCAAGCACCCGGTG CTCGTCGTCGGAGCGGCCGCGATCCGCTCGGGCGCCGTCCCGGCGATCCGCGCCCTGGCC GAGCGCCTGAACATCCCGGTCATCACGACCTACATCGCCAAGGGTGTCCTGCCGGTCGGC CACGAGCTGAACTACGGCGCCGTCACCGGCTACATGGACGGCATCCTCAACTTCCCGGCG CTCCAGACCATGTTCGCCCCGGTGGACCTCGTCCTCACCGTCGGCTACGACTACGCCGAG GACCTGCGCCCGTCCATGTGGCAGAAGGGCATCGAGAAGAAGACCGTCCGTATCTCCCCG ACGGTCAACCCGATCCCCCGGGTCTACCGGCCCGACGTCGACGTCGTCACCGACGTCCTC GCCTTCGTGGAGCACTTCGAGACCGCGACCGCCTCCTTCGGGGCCAAGCAGCGCCACGAC ATCGAGCCGCTGCGCGCCCGGATCGCGGAGTTCCTGGCCGACCCGGAGACCTACGAGGAC GGCATGCGCGTCCACCAGGTCATCGACTCCATGAACACCGTCATGGAGGAGGCCGCCGAG CCCGGCGAGGGCACGATCGTCTCCGACATCGGCTTCTTCCGTCACTACGGTGTGCTCTTC GCCCGCGCCGACCAGCCCTTCGGCTTCCTCACCTCGGCGGGCTGCTCCAGCTTCGGCTAC GGCATCCCCGCCGCCATCGGCGCCCAGATGGCCCGCCCGGACCAGCCGACCTTCCTCATC GCGGGTGACGGCGGCTTCCACTCCAACAGCTCCGACCTGGAGACCATCGCCCGGCTCAAC CTGCCGATCGTGACCGTCGTCGTCAACAACGACACCAACGGCCTGATCGAGCTGTACCAG AACATCGGTCACCACCGCAGCCACGACCCGGCGGTCAAGTTCGGCGGCGTCGACTTCGTC GCGCTCGCCGAGGCCAACGGTGTCGACGCCACCCGCGCCACCAACCGCGAGGAGCTGCTC GCGGCCCTGCGCAAGGGTGCCGAGCTGGGTCGTCCGTTCCTCATCGAGGTCCCGGTCAAC TACGACTTCCAGCCGGGCGGCTTCGGCGCCCTGAGCATCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9LCV9 UniProtKB Entry Name CEAS_STRCL GenBank Gene ID U87786 - General References
- Aidoo KA, Wong A, Alexander DC, Rittammer RA, Jensen SE: Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis. Gene. 1994 Sep 15;147(1):41-6. [Article]
- Paradkar AS, Aidoo KA, Wong A, Jensen SE: Molecular analysis of a beta-lactam resistance gene encoded within the cephamycin gene cluster of Streptomyces clavuligerus. J Bacteriol. 1996 Nov;178(21):6266-74. [Article]
- Paradkar AS, Aidoo KA, Jensen SE: A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus. Mol Microbiol. 1998 Feb;27(4):831-43. [Article]
- Jensen SE, Elder KJ, Aidoo KA, Paradkar AS: Enzymes catalyzing the early steps of clavulanic acid biosynthesis are encoded by two sets of paralogous genes in Streptomyces clavuligerus. Antimicrob Agents Chemother. 2000 Mar;44(3):720-6. [Article]
- Merski M, Townsend CA: Observation of an acryloyl-thiamin diphosphate adduct in the first step of clavulanic acid biosynthesis. J Am Chem Soc. 2007 Dec 26;129(51):15750-1. Epub 2007 Dec 5. [Article]
- Caines ME, Elkins JM, Hewitson KS, Schofield CJ: Crystal structure and mechanistic implications of N2-(2-carboxyethyl)arginine synthase, the first enzyme in the clavulanic acid biosynthesis pathway. J Biol Chem. 2004 Feb 13;279(7):5685-92. Epub 2003 Nov 17. [Article]
- Caines ME, Sorensen JL, Schofield CJ: Structural and mechanistic studies on N(2)-(2-carboxyethyl)arginine synthase. Biochem Biophys Res Commun. 2009 Aug 7;385(4):512-7. doi: 10.1016/j.bbrc.2009.05.095. Epub 2009 May 27. [Article]