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Cruzipain

ID 2368
Name Cruzipain
Synonyms
  • EC 3.4.22.51
  • Major cysteine proteinase
  • Cruzaine
  • Cruzipain precursor
Gene Name Not Available
Protein Sequence 
>Cruzipain precursor
MSGWARALLLAAVLVVMACLVPAATASLHAEETLTSQFAEFKQKHGRVYESAAEEAFRLS
VFRENLFLARLHAAANPHATFGVTPFSDLTREEFRSRYHNGAAHFAAAQERARVPVKVEV
VGAPAAVDWRARGAVTAVKDQGQCGSCWAFSAIGNVECQWFLAGHPLTNLSEQMLVSCDK
TDSGCSGGLMNNAFEWIVQENNGAVYTEDSYPYASGEGISPPCTTSGHTVGATITGHVEL
PQDEAQIAAWLAVNGPVAVAVDASSWMTYTGGVMTSCVSEQLDHGVLLVGYNDSAAVPYW
IIKNSWTTQWGEEGYIRIAKGSNQCLVKEEASSAVVGGPGPTPEPTTTTTTSAPGPSPSY
FVQMSCTDAACIVGCENVTLPTGQCLLTTSGVSAIVTCGAETLTEEVFLTSTHCSGPSVR
SSVPLNKCNRLLRGSVEFFCGSSSSGRLADVDRQRRHQPYHSRHRRL
Number of Residues 467
Molecular Weight 49836
Theoretical pI 6.01
GO Classification
Function
cysteine-type endopeptidase activity
cysteine-type peptidase activity
catalytic activity
hydrolase activity
peptidase activity
endopeptidase activity
Process
physiological process
metabolism
macromolecule metabolism
protein metabolism
cellular protein metabolism
proteolysis
General Function Involved in cysteine-type endopeptidase activity
Specific Function The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle
Pathways Not Available
Reactions
  • Broad endopeptidase specificity similar to that of cathepsin L
Pfam Domain Function
Signals 1-18
Transmembrane Regions None
Essentiality Non-Essential
GenBank Protein ID 162048 Link_out
UniProtKB ID P25779 Link_out
UniProtKB Entry Name CYSP_TRYCR Link_out
PDB ID 1U9Q Link_out
PDB File show
3D Structure View 3D Structure
Cellular Location Cytoplasmic
Gene Sequence 
>1404 bp
ATGTCTGGCTGGGCGCGTGCGCTGTTGCTCGCGGCCGTCCTGGTCGTCATGGCGTGCCTC
GTCCCCGCGGCGACGGCGAGCCTGCATGCGGAGGAGACGCTGACGTCGCAATTCGCAGAA
TTCAAGCAGAAGCATGGCAGGGTGTACGAGAGCGCCGCGGAGGAGGCGTTCCGCCTGAGC
GTGTTCAGGGAGAACCTGTTTCTTGCGAGGCTGCACGCCGCGGCAAACCCACACGCGACC
TTCGGCGTCACGCCCTTCTCGGACCTCACGCGCGAGGAATTCCGGTCCCGCTACCACAAC
GGCGCGGCGCACTTTGCGGCGGCGCAGGAGCGCGCGAGAGTGCCGGTGAAGGTGGAGGTA
GTTGGCGCGCCCGCGGCAGTGGATTGGCGTGCGAGAGGCGCCGTGACAGCCGTCAAGGAC
CAGGGCCAATGCGGTTCGTGCTGGGCCTTCTCCGCCATTGGCAACGTTGAGTGCCAGTGG
TTTCTTGCCGGCCACCCGCTGACGAACCTGTCGGAGCAGATGCTCGTGTCGTGCGACAAA
ACGGACTCTGGCTGCAGTGGTGGCCTGATGAACAACGCCTTTGAGTGGATTGTGCAGGAG
AATAACGGCGCCGTGTACACGGAGGACAGCTACCCTTATGCGTCGGGCGAGGGGATATCG
CCGCCGTGCACGACGTCAGGCCACACGGTGGGTGCCACGATTACCGGTCACGTTGAATTA
CCTCAGGACGAGGCCCAAATAGCCGCATGGCTTGCAGTCAATGGCCCGGTTGCCGTTGCC
GTCGACGCCAGCAGCTGGATGACCTACACGGGCGGCGTTATGACGAGCTGCGTCTCCGAG
CAGCTGGATCACGGCGTTCTTCTCGTCGGCTACAATGACAGCGCCGCAGTGCCGTACTGG
ATCATCAAGAACTCGTGGACCACGCAGTGGGGCGAGGAAGGCTACATCCGCATTGCAAAG
GGCTCGAACCAGTGCCTTGTCAAGGAGGAGGCGAGCTCCGCGGTGGTCGGTGGTCCCGGA
CCCACTCCCGAGCCAACCACCACGACAACCACAAGTGCCCCAGGACCGTCCCCATCGTAC
TTTGTGCAGATGTCCTGCACTGACGCTGCGTGCATTGTCGGGTGCGAGAACGTGACGTTA
CCGACCGGTCAGTGTCTCCTGACCACCAGCGGCGTCTCTGCCATTGTCACGTGCGGTGCT
GAGACTCTCACAGAAGAAGTCTTCCTTACGAGTACGCACTGCAGCGGCCCATCGGTGAGG
TCCTCTGTTCCTCTCAACAAATGCAACCGGCTTTTAAGAGGCTCCGTTGAGTTCTTCTGC
GGCTCCAGCTCCAGTGGCCGACTGGCCGACGTGGACAGGCAGCGTCGCCATCAGCCATAC
CACAGCCGTCATCGCCGCCTCTGA
GenBank Gene ID M84342 Link_out
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
HPRD ID Not Available
IUPHAR ID Not Available
Guide to Pharmacology ID Not Available
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
General References
  1. Eakin AE, Mills AA, Harth G, McKerrow JH, Craik CS: The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi. J Biol Chem. 1992 Apr 15;267(11):7411-20. Pubmed
  2. Campetella O, Henriksson J, Aslund L, Frasch AC, Pettersson U, Cazzulo JJ: The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes. Mol Biochem Parasitol. 1992 Feb;50(2):225-34. Pubmed
  3. Eakin AE, Bouvier J, Sakanari JA, Craik CS, McKerrow JH: Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites. Mol Biochem Parasitol. 1990 Feb;39(1):1-8. Pubmed
  4. Aslund L, Henriksson J, Campetella O, Frasch AC, Pettersson U, Cazzulo JJ: The C-terminal extension of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi. Mol Biochem Parasitol. 1991 Apr;45(2):345-7. Pubmed
  5. Hellman U, Wernstedt C, Cazzulo JJ: Self-proteolysis of the cysteine proteinase, cruzipain, from Trypanosoma cruzi gives a major fragment corresponding to its carboxy-terminal domain. Mol Biochem Parasitol. 1991 Jan;44(1):15-21. Pubmed
  6. Cazzulo JJ, Cazzulo Franke MC, Martinez J, Franke de Cazzulo BM: Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi. Biochim Biophys Acta. 1990 Feb 9;1037(2):186-91. Pubmed
  7. Cazzulo JJ, Couso R, Raimondi A, Wernstedt C, Hellman U: Further characterization and partial amino acid sequence of a cysteine proteinase from Trypanosoma cruzi. Mol Biochem Parasitol. 1989 Feb;33(1):33-41. Pubmed
  8. Gillmor SA, Craik CS, Fletterick RJ: Structural determinants of specificity in the cysteine protease cruzain. Protein Sci. 1997 Aug;6(8):1603-11. Pubmed
Drugs
# DrugBank ID Drug Name Drug Type Drug Groups
1 DB01871 [1-(1-Benzyl-3-Hydroxy-2-Oxo-Propylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester small molecule experimental
2 DB01810 [1-(1-Methyl-4,5-Dioxo-Pent-2-Enylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester small molecule experimental
3 DB02128 [1-(3-Hydroxy-2-Oxo-1-Phenethyl-Propylcarbamoyl)2-Phenyl-Ethyl]-Carbamic Acid Pyridin-4-Ylmethyl Ester small molecule experimental
4 DB04427 3-[[N-[4-Methyl-Piperazinyl]Carbonyl]-Phenylalaninyl-Amino]-5-Phenyl-Pentane-1-Sulfonic Acid Benzyloxy-Amide small molecule experimental
5 DB02051 3-[N-[Benzyloxycarbonyl]-Phenylalaninyl-Amino]-5-Phenyl-Pentane-1-Sulfonic Acid 4-Nitro-Phenyl Ester small molecule experimental
6 DB02200 3-[N-[Benzyloxycarbonyl]-Phenylalaninyl-Amino]-5-Phenyl-Pentane-1-Sulfonylmethylbenzene small molecule experimental
7 DB03536 Benzoyl-Arginine-Alanine-Methyl Ketone small molecule experimental
8 DB03691 WRR-112 small molecule experimental
9 DB04502 WRR-204 small molecule experimental
10 DB03573 WRR-99 small molecule experimental