Banner

KHG/KDPG aldolase [Includes: 4-hydroxy-2-oxoglutarate aldolase

ID 3792
Name KHG/KDPG aldolase [Includes: 4-hydroxy-2-oxoglutarate aldolase
Synonyms
  • EC 4.1.3.16
  • 2-keto-4-hydroxyglutarate aldolase
  • KHG-aldolase
  • 2- dehydro-3-deoxy-phosphogluconate aldolase
  • EC 4.1.2.14
  • Phospho-2- dehydro-3-deoxygluconate aldolase
  • Phospho-2-keto-3-deoxygluconate aldolase
  • 2-keto-3-deoxy-6-phosphogluconate aldolase
  • KDPG- aldolase]
Gene Name eda
Protein Sequence 
>KHG/KDPG aldolase [Includes: 4-hydroxy-2-oxoglutarate aldolase
MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAI
AKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSE
LMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLC
IGGSWLVPADALEAGDYDRITKLAREAVEGAKL
Number of Residues 213
Molecular Weight 22284
Theoretical pI 5.35
GO Classification
Function
catalytic activity
Process
metabolism
physiological process
General Function Carbohydrate transport and metabolism
Specific Function 4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Pathways Not Available
Reactions
  • 4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Pfam Domain Function
Signals None
Transmembrane Regions None
Essentiality Non-Essential
GenBank Protein ID Not Available
UniProtKB ID P0A955 Link_out
UniProtKB Entry Name ALKH_ECOLI Link_out
PDB ID 1FQ0 Link_out
PDB File show
3D Structure View 3D Structure
Cellular Location Cytoplasm
Gene Sequence Not Available
GenBank Gene ID X68871 Link_out
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
HPRD ID Not Available
IUPHAR ID Not Available
Guide to Pharmacology ID Not Available
Chromosome Location Not Available
Locus Not Available
SNPs eda Link_out
General References
  1. Vlahos CJ, Dekker EE: The complete amino acid sequence and identification of the active-site arginine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase. J Biol Chem. 1988 Aug 25;263(24):11683-91. Pubmed
  2. Patil RV, Dekker EE: Cloning, nucleotide sequence, overexpression, and inactivation of the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene. J Bacteriol. 1992 Jan;174(1):102-7. Pubmed
  3. Egan SE, Fliege R, Tong S, Shibata A, Wolf RE Jr, Conway T: Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon. J Bacteriol. 1992 Jul;174(14):4638-46. Pubmed
  4. Carter AT, Pearson BM, Dickinson JR, Lancashire WE: Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway. Gene. 1993 Aug 16;130(1):155-6. Pubmed
  5. Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T, et al.: A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):379-92. Pubmed
  6. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-74. Pubmed
  7. Vlahos CJ, Dekker EE: Active-site residues of 2-keto-4-hydroxyglutarate aldolase from Escherichia coli. Bromopyruvate inactivation and labeling of glutamate 45. J Biol Chem. 1990 Nov 25;265(33):20384-9. Pubmed
  8. Wymer N, Buchanan LV, Henderson D, Mehta N, Botting CH, Pocivavsek L, Fierke CA, Toone EJ, Naismith JH: Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. Structure. 2001 Jan 10;9(1):1-9. Pubmed
  9. Allard J, Grochulski P, Sygusch J: Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) aldolase structure at 1.95-A resolution. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3679-84. Pubmed
Drugs
# DrugBank ID Drug Name Drug Type Drug Groups
1 DB04272 Citric Acid small molecule experimental