Serine/threonine-protein kinase RIO3

Details

Name

Serine/threonine-protein kinase RIO3

Synonyms

• 2.7.11.1
• RIO kinase 3
• SUDD
• sudD homolog

Gene Name

RIOK3

Organism

Humans

Amino acid sequence

>lcl|BSEQ0051938|Serine/threonine-protein kinase RIO3
MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVA
VAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVH
PYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARME
NFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLL
MYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFK
NRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMS
FIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVW
LIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEAD
FLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

Number of residues

519

Molecular Weight

59092.58

Theoretical pI

Not Available

GO Classification

Functions

ATP binding / caspase binding / metal ion binding / protein serine/threonine kinase activity

Processes

cellular response to dsDNA / cellular response to dsRNA / cellular response to virus / chromosome segregation / defense response to virus / innate immune response / maturation of SSU-rRNA / negative regulation of I-kappaB kinase/NF-kappaB signaling / negative regulation of MDA-5 signaling pathway / negative regulation of protein homooligomerization / positive regulation of innate immune response / positive regulation of interferon-beta production / rRNA processing

Components

cytosol / preribosome, small subunit precursor

General Function

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed:24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed:25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway (PubMed:19557502). May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA (PubMed:22418843).

Specific Function

Atp binding

Pfam Domain Function

Not Available

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0051939|Serine/threonine-protein kinase RIO3 (RIOK3)
ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGC
AAGTGTCCATGGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGT
GAACAGCTGGCCAAAGAATTGCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCT
GTTGCTGAAGGACCATTTATTACTGGAGAAAACATTGATACTTCCAGTGACCTTATGCTG
GCTCAGATGCTACAGATGGAATATGACAGAGAATATGATGCACAGCTTAGGCGTGAAGAA
AAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCGAAAAGTGCAT
CCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT
CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAA
GATATCACCACCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAA
AATTTTGCACCTGAGTTTCAGGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAAC
CATGTTTTCAATGCTTTAAAACAACATGCCTACTCAGAAGAACGTCGAAGTGCCCGCCTA
CATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTGATCCTAAGACACGTTTACTT
ATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTATTAGTACAGGA
AAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT
AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAG
AATCGTGACAAATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAAT
CCACGTAAGATCATCCGCATGTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAG
AGAGCTGGAATTCCTTGTCCAACAGTTGTACTACTGAAGAAACACATTTTAGTTATGTCT
TTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAGAAGTAAAGCTCAATAGTGAA
GAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATATCATGAATGT
ACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTCTGG
TTGATCGATGTCAGTCAGTCAGTAGAACCTACCCACCCTCACGGCCTGGAGTTCTTGTTC
CGGGACTGCAGGAATGTCTCGCAGTTTTTCCAGAAAGGAGGAGTCAAGGAAGCCCTTAGT
GAACGAGAACTCTTCAATGCTGTTTCAGGCTTAAACATCACAGCAGATAATGAAGCTGAT
TTTTTAGCTGAGATAGAAGCTTTGGAGAAAATGAATGAAGATCACGTTCAGAAGAATGGA
AGGAAAGCTGCTTCATTTTTGAAAGATGATGGAGACCCACCACTACTATATGATGAATAG

Chromosome Location

18

Locus

18q11.2

External Identifiers

Resource	Link
UniProtKB ID	O14730
UniProtKB Entry Name	RIOK3_HUMAN
HGNC ID	HGNC:11451

General References

1. Anaya P, Evans SC, Dai C, Lozano G, May GS: Isolation of the Aspergillus nidulans sudD gene and its human homologue. Gene. 1998 May 12;211(2):323-9. [Article]
2. Nusbaum C, Zody MC, Borowsky ML, Kamal M, Kodira CD, Taylor TD, Whittaker CA, Chang JL, Cuomo CA, Dewar K, FitzGerald MG, Yang X, Abouelleil A, Allen NR, Anderson S, Bloom T, Bugalter B, Butler J, Cook A, DeCaprio D, Engels R, Garber M, Gnirke A, Hafez N, Hall JL, Norman CH, Itoh T, Jaffe DB, Kuroki Y, Lehoczky J, Lui A, Macdonald P, Mauceli E, Mikkelsen TS, Naylor JW, Nicol R, Nguyen C, Noguchi H, O'Leary SB, O'Neill K, Piqani B, Smith CL, Talamas JA, Topham K, Totoki Y, Toyoda A, Wain HM, Young SK, Zeng Q, Zimmer AR, Fujiyama A, Hattori M, Birren BW, Sakaki Y, Lander ES: DNA sequence and analysis of human chromosome 18. Nature. 2005 Sep 22;437(7058):551-5. [Article]
3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
4. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [Article]
5. Shan J, Wang P, Zhou J, Wu D, Shi H, Huo K: RIOK3 interacts with caspase-10 and negatively regulates the NF-kappaB signaling pathway. Mol Cell Biochem. 2009 Dec;332(1-2):113-20. doi: 10.1007/s11010-009-0180-8. Epub 2009 Jun 26. [Article]
6. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
7. Widmann B, Wandrey F, Badertscher L, Wyler E, Pfannstiel J, Zemp I, Kutay U: The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits. Mol Biol Cell. 2012 Jan;23(1):22-35. doi: 10.1091/mbc.E11-07-0639. Epub 2011 Nov 9. [Article]
8. Baumas K, Soudet J, Caizergues-Ferrer M, Faubladier M, Henry Y, Mougin A: Human RioK3 is a novel component of cytoplasmic pre-40S pre-ribosomal particles. RNA Biol. 2012 Feb;9(2):162-74. doi: 10.4161/rna.18810. Epub 2012 Feb 1. [Article]
9. Zhou H, Di Palma S, Preisinger C, Peng M, Polat AN, Heck AJ, Mohammed S: Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res. 2013 Jan 4;12(1):260-71. doi: 10.1021/pr300630k. Epub 2012 Dec 18. [Article]
10. Feng J, De Jesus PD, Su V, Han S, Gong D, Wu NC, Tian Y, Li X, Wu TT, Chanda SK, Sun R: RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. J Virol. 2014 Jul;88(14):7987-97. doi: 10.1128/JVI.00643-14. Epub 2014 May 7. [Article]
11. Takashima K, Oshiumi H, Takaki H, Matsumoto M, Seya T: RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell Rep. 2015 Apr 14;11(2):192-200. doi: 10.1016/j.celrep.2015.03.027. [Article]
12. Greenman C, Stephens P, Smith R, Dalgliesh GL, Hunter C, Bignell G, Davies H, Teague J, Butler A, Stevens C, Edkins S, O'Meara S, Vastrik I, Schmidt EE, Avis T, Barthorpe S, Bhamra G, Buck G, Choudhury B, Clements J, Cole J, Dicks E, Forbes S, Gray K, Halliday K, Harrison R, Hills K, Hinton J, Jenkinson A, Jones D, Menzies A, Mironenko T, Perry J, Raine K, Richardson D, Shepherd R, Small A, Tofts C, Varian J, Webb T, West S, Widaa S, Yates A, Cahill DP, Louis DN, Goldstraw P, Nicholson AG, Brasseur F, Looijenga L, Weber BL, Chiew YE, DeFazio A, Greaves MF, Green AR, Campbell P, Birney E, Easton DF, Chenevix-Trench G, Tan MH, Khoo SK, Teh BT, Yuen ST, Leung SY, Wooster R, Futreal PA, Stratton MR: Patterns of somatic mutation in human cancer genomes. Nature. 2007 Mar 8;446(7132):153-8. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB12010	Fostamatinib	approved, investigational	unknown	inhibitor	Details