Cystathionine gamma-synthase
Details
- Name
- Cystathionine gamma-synthase
- Synonyms
- 2.5.1.48
- CGS
- O-succinylhomoserine (thiol)-lyase
- Gene Name
- metB
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0020661|Cystathionine gamma-synthase MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRA LAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRVL FVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKICHLAREVGAVSVVDNTFLSPA LQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDVVTELAWWANNIGVTGGAFDSYL LLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLYHPSLPENQGHEIAARQQKGFGAML SFELDGDEQTLRRFLGGLSLFTLAESLGGVESLISHAATMTHAGMAPEARAAAGISETLL RISTGIEDGEDLIADLENGFRAANKG
- Number of residues
- 386
- Molecular Weight
- 41549.995
- Theoretical pI
- 6.44
- GO Classification
- Functionscystathionine gamma-synthase activity / pyridoxal phosphate bindingProcessesmethionine biosynthetic processComponentscytoplasm
- General Function
- Pyridoxal phosphate binding
- Specific Function
- Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia.
- Pfam Domain Function
- Cys_Met_Meta_PP (PF01053)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0020662|Cystathionine gamma-synthase (metB) ATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTAT GGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAA CCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCG CTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATT CACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGC TACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTG TTTGTTGATCAAGGCGATGAACAGGCATTACGGGCAGCGCTGGCAGAAAAACCCAAACTG GTACTGGTAGAAAGCCCAAGTAATCCATTGTTACGCGTCGTGGATATTGCGAAAATCTGC CATCTGGCAAGGGAAGTCGGGGCGGTGAGCGTGGTGGATAACACCTTCTTAAGCCCGGCA TTACAAAATCCGCTGGCATTAGGTGCCGATCTGGTGTTGCATTCATGCACGAAATATCTG AACGGTCACTCAGACGTAGTGGCCGGCGTGGTGATTGCTAAAGACCCGGACGTTGTCACT GAACTGGCCTGGTGGGCAAACAATATTGGCGTGACGGGCGGCGCGTTTGACAGCTATCTG CTGCTACGTGGGTTGCGAACGCTGGTGCCGCGTATGGAGCTGGCGCAGCGCAACGCGCAG GCGATTGTGAAATACCTGCAAACCCAGCCGTTGGTGAAAAAACTGTATCACCCGTCGTTG CCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTG AGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTG TTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATG ACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTG CGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTC CGGGCTGCAAACAAGGGGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P00935 UniProtKB Entry Name METB_ECOLI GenBank Gene ID K01546 - General References
- Duchange N, Zakin MM, Ferrara P, Saint-Girons I, Park I, Tran SV, Py MC, Cohen GN: Structure of the metJBLF cluster in Escherichia coli K12. Sequence of the metB structural gene and of the 5'- and 3'-flanking regions of the metBL operon. J Biol Chem. 1983 Dec 25;258(24):14868-71. [Article]
- Plunkett G 3rd, Burland V, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 1993 Jul 25;21(15):3391-8. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Martel A, Bouthier de la Tour C, Le Goffic F: Pyridoxal 5'phosphate binding site of Escherichia coli beta cystathionase and cystathionine gamma synthase comparison of their sequences. Biochem Biophys Res Commun. 1987 Sep 15;147(2):565-71. [Article]
- Holbrook EL, Greene RC, Krueger JH: Purification and properties of cystathionine gamma-synthase from overproducing strains of Escherichia coli. Biochemistry. 1990 Jan 16;29(2):435-42. [Article]
- VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
- Clausen T, Huber R, Prade L, Wahl MC, Messerschmidt A: Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution. EMBO J. 1998 Dec 1;17(23):6827-38. [Article]