HTH-type transcriptional regulator QacR
Details
- Name
- HTH-type transcriptional regulator QacR
- Synonyms
- Not Available
- Gene Name
- qacR
- Organism
- Staphylococcus aureus
- Amino acid sequence
>lcl|BSEQ0012752|HTH-type transcriptional regulator QacR MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFKTKENLFLEILNIEESK WQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEFYTEYYKTNSINEKMNKLE NKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIVTFTHEQNINERIKLMNKFSQ IFLNGLSK
- Number of residues
- 188
- Molecular Weight
- 22174.175
- Theoretical pI
- 8.55
- GO Classification
- FunctionsDNA binding / transcription factor activity, sequence-specific DNA bindingProcessesnegative regulation of transcription, DNA-templated / transcription, DNA-templated
- General Function
- Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
- Specific Function
- Dna binding
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0012753|PG:9487076 QACR, PSK1_P27; STAPHYLOCOCCUS AUREUS PLASMID PSK1; ORF188; SIMILAR TO KNOWN REGULATORS SUCH AS TET REGULATOR TN10; K18938 TETR/ACRR FAMILY TRANSCRIPTIONAL REGULATOR, REPRESSOR FOR QACA (N) ATGAACTTGAAAGATAAAATACTAGGTGTCGCAAAGGAATTATTTATAAAAAATGGATAT AATGCCACTACTACTGGAGAAATTGTTAAATTATCAGAAAGTAGTAAAGGGAATCTTTAT TATCACTTTAAAACAAAAGAAAATCTATTTTTAGAAATTTTAAATATAGAAGAATCTAAA TGGCAAGAACAGTGGAAAAAGGAACAAATCAAATGTAAAACTAATAGAGAAAAATTTTAT TTATATAATGAACTATCTTTAACAACCGAATACTATTATCCACTTCAAAACGCAATAATT GAATTTTACACTGAATATTATAAAACTAATAGCATTAATGAAAAAATGAATAAATTAGAA AACAAATATATAGATGCTTATCATGTAATTTTTAAGGAGGGTAATTTAAATGGCGAATGG TGTATTAATGATGTTAATGCTGTTAGTAAAATAGCAGCAAACGCTGTTAATGGAATTGTT ACCTTTACACATGAACAAAATATTAATGAAAGAATTAAACTTATGAACAAGTTCTCTCAA ATTTTTTTAAATGGACTTAGTAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0A0N4 UniProtKB Entry Name QACR_STAAU GenBank Protein ID 46660 GenBank Gene ID X56628 - General References
- Rouch DA, Cram DS, DiBerardino D, Littlejohn TG, Skurray RA: Efflux-mediated antiseptic resistance gene qacA from Staphylococcus aureus: common ancestry with tetracycline- and sugar-transport proteins. Mol Microbiol. 1990 Dec;4(12):2051-62. [Article]
- Jensen SO, Apisiridej S, Kwong SM, Yang YH, Skurray RA, Firth N: Analysis of the prototypical Staphylococcus aureus multiresistance plasmid pSK1. Plasmid. 2010 Nov;64(3):135-42. doi: 10.1016/j.plasmid.2010.06.001. Epub 2010 Jun 12. [Article]
- Grkovic S, Brown MH, Roberts NJ, Paulsen IT, Skurray RA: QacR is a repressor protein that regulates expression of the Staphylococcus aureus multidrug efflux pump QacA. J Biol Chem. 1998 Jul 17;273(29):18665-73. [Article]
- Grkovic S, Brown MH, Schumacher MA, Brennan RG, Skurray RA: The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers. J Bacteriol. 2001 Dec;183(24):7102-9. [Article]
- Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG: Structural mechanisms of QacR induction and multidrug recognition. Science. 2001 Dec 7;294(5549):2158-63. [Article]
- Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG: Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR. EMBO J. 2002 Mar 1;21(5):1210-8. [Article]
- Schumacher MA, Brennan RG: Deciphering the molecular basis of multidrug recognition: crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR. Res Microbiol. 2003 Mar;154(2):69-77. [Article]