pH-gated potassium channel KcsA
Details
- Name
- pH-gated potassium channel KcsA
- Synonyms
- skc1
- Streptomyces lividans K+ channel
- Gene Name
- kcsA
- Organism
- Streptomyces lividans
- Amino acid sequence
>lcl|BSEQ0010947|pH-gated potassium channel KcsA MPPMLSGLLARLVKLLLGRHGSALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLI TYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGREQE RRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR
- Number of residues
- 160
- Molecular Weight
- 17693.465
- Theoretical pI
- 10.66
- GO Classification
- Functionsidentical protein binding / voltage-gated potassium channel activityComponentsvoltage-gated potassium channel complex
- General Function
- Voltage-gated potassium channel activity
- Specific Function
- Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).
- Pfam Domain Function
- Ion_trans_2 (PF07885)
- Transmembrane Regions
- 28-50 88-111
- Cellular Location
- Cell membrane
- Gene sequence
>lcl|BSEQ0002690|483 bp ATGCCACCCATGCTGTCCGGTCTTCTGGCCAGATTGGTCAAACTGCTGCTCGGGCGCCAC GGCAGTGCGCTGCACTGGAGGGCCGCGGGTGCCGCGACGGTCCTCCTGGTGATCGTCCTC CTCGCGGGCTCGTACTTGGCCGTCCTGGCTGAGCGCGGCGCACCGGGCGCGCAGCTGATC ACGTATCCGCGGGCGCTGTGGTGGTCCGTGGAGACCGCGACGACCGTCGGCTACGGCGAC CTGTACCCCGTGACTCTGTGGGGCCGGCTCGTGGCCGTGGTGGTGATGGTCGCCGGGATC ACCTCCTTCGGTCTGGTGACCGCCGCGCTGGCCACCTGGTTCGTCGGCCGGGAACAAGAG CGCCGGGGCCACTTCGTGCGCCACTCCGAGAAGGCCGCCGAGGAGGCGTACACGCGGACG ACCCGGGCGCTGCACGAGCGTTTCGACCGTTTGGAGCGAATGCTCGACGACAACCGCCGG TGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0A334 UniProtKB Entry Name KCSA_STRLI GenBank Protein ID 1089906 GenBank Gene ID Z37969 - General References
- Schrempf H, Schmidt O, Kummerlen R, Hinnah S, Muller D, Betzler M, Steinkamp T, Wagner R: A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 1995 Nov 1;14(21):5170-8. [Article]
- Cuello LG, Romero JG, Cortes DM, Perozo E: pH-dependent gating in the Streptomyces lividans K+ channel. Biochemistry. 1998 Mar 10;37(10):3229-36. [Article]
- le Coutre J, Whitelegge JP, Gross A, Turk E, Wright EM, Kaback HR, Faull KF: Proteomics on full-length membrane proteins using mass spectrometry. Biochemistry. 2000 Apr 18;39(15):4237-42. [Article]
- Hirano M, Takeuchi Y, Aoki T, Yanagida T, Ide T: Rearrangements in the KcsA cytoplasmic domain underlie its gating. J Biol Chem. 2010 Feb 5;285(6):3777-83. doi: 10.1074/jbc.M109.084368. Epub 2009 Dec 3. [Article]
- Gouaux E: Single potassium ion seeks open channel for transmembrane travels: tales from the KcsA structure. Structure. 1998 Oct 15;6(10):1221-6. [Article]
- Doyle DA, Morais Cabral J, Pfuetzner RA, Kuo A, Gulbis JM, Cohen SL, Chait BT, MacKinnon R: The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science. 1998 Apr 3;280(5360):69-77. [Article]
- Cortes DM, Cuello LG, Perozo E: Molecular architecture of full-length KcsA: role of cytoplasmic domains in ion permeation and activation gating. J Gen Physiol. 2001 Feb;117(2):165-80. [Article]
- Liu YS, Sompornpisut P, Perozo E: Structure of the KcsA channel intracellular gate in the open state. Nat Struct Biol. 2001 Oct;8(10):883-7. [Article]
- Morais-Cabral JH, Zhou Y, MacKinnon R: Energetic optimization of ion conduction rate by the K+ selectivity filter. Nature. 2001 Nov 1;414(6859):37-42. [Article]
- Zhou Y, Morais-Cabral JH, Kaufman A, MacKinnon R: Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution. Nature. 2001 Nov 1;414(6859):43-8. [Article]
- Uysal S, Vasquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A: Crystal structure of full-length KcsA in its closed conformation. Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6644-9. doi: 10.1073/pnas.0810663106. Epub 2009 Apr 3. [Article]
- Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E: Mechanism of activation gating in the full-length KcsA K+ channel. Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):11896-9. doi: 10.1073/pnas.1105112108. Epub 2011 Jul 5. [Article]