Glutathione S-transferase GstA

Details

Name
Glutathione S-transferase GstA
Synonyms
  • 2.5.1.18
  • gst
  • GST B1-1
Gene Name
gstA
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0019227|Glutathione S-transferase GstA
MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYFAVNPKGQVPALLLDDG
TLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEE
YKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAA
FMQRMAERPEVQDALSAEGLK
Number of residues
201
Molecular Weight
22868.14
Theoretical pI
6.13
GO Classification
Functions
glutathione transferase activity
Processes
glutathione metabolic process / xenobiotic catabolic process
Components
cytoplasm
General Function
Glutathione transferase activity
Specific Function
Catalyzes the conjugation of reduced glutathione (GSH) to a wide number of exogenous and endogenous hydrophobic electrophiles. Shows activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid. Also possesses thiol:disulfide oxidoreductase activity, using GSH to reduce bis-(2-hydroxyethyl) disulfide (HEDS). Has a low level of glutathione-dependent peroxidase activity toward cumene hydroperoxide. Is important for defense against oxidative stress, probably via its peroxidase activity.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0019228|Glutathione S-transferase GstA (gstA)
ATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAG
AGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGT
GACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGT
ACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGC
CAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTAC
ATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAG
TACAAACCGACAGTTCGCGCGCAGCTGGAGAAGAAGCTGCAATATGTGAACGAGGCACTG
AAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACG
GTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCA
TTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTA
AAGTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP0A9D2
UniProtKB Entry NameGSTA_ECOLI
GenBank Gene IDD38497
General References
  1. Nishida M, Kong KH, Inoue H, Takahashi K: Molecular cloning and site-directed mutagenesis of glutathione S-transferase from Escherichia coli. The conserved tyrosyl residue near the N terminus is not essential for catalysis. J Biol Chem. 1994 Dec 23;269(51):32536-41. [Article]
  2. Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Arca P, Garcia P, Hardisson C, Suarez JE: Purification and study of a bacterial glutathione S-transferase. FEBS Lett. 1990 Apr 9;263(1):77-9. [Article]
  6. Kanai T, Takahashi K, Inoue H: Three distinct-type glutathione S-transferases from Escherichia coli important for defense against oxidative stress. J Biochem. 2006 Nov;140(5):703-11. Epub 2006 Oct 3. [Article]
  7. Wang XY, Zhang ZR, Perrett S: Characterization of the activity and folding of the glutathione transferase from Escherichia coli and the roles of residues Cys(10) and His(106). Biochem J. 2009 Jan 1;417(1):55-64. doi: 10.1042/BJ20071702. [Article]
  8. Nishida M, Harada S, Noguchi S, Satow Y, Inoue H, Takahashi K: Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: catalytic roles of Cys10 and His106. J Mol Biol. 1998 Aug 7;281(1):135-47. [Article]
  9. Rife CL, Parsons JF, Xiao G, Gilliland GL, Armstrong RN: Conserved structural elements in glutathione transferase homologues encoded in the genome of Escherichia coli. Proteins. 2003 Dec 1;53(4):777-82. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB03003Glutathione sulfonic acidexperimentalunknownDetails