Respiratory nitrate reductase 1 gamma chain
Details
- Name
- Respiratory nitrate reductase 1 gamma chain
- Synonyms
- 1.7.99.4
- chlI
- Cytochrome B-NR
- Nitrate reductase A subunit gamma
- Quinol-nitrate oxidoreductase subunit gamma
- Gene Name
- narI
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0017233|Respiratory nitrate reductase 1 gamma chain MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLDRKGMNLASNLFHIGIL GIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCLIGGVLLLKRRLFSPRVRA TTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLVGWAQSVVTFHGGASQHLDGV AFIFRLHLVLGMTLFLLFPFSRLIHIWSVPVEYLTRKYQLVRARH
- Number of residues
- 225
- Molecular Weight
- 25497.16
- Theoretical pI
- 10.11
- GO Classification
- Functionselectron carrier activity / heme binding / metal ion binding / nitrate reductase activityProcessesanaerobic electron transport chain / anaerobic respiration / nitrate assimilationComponentsintegral component of plasma membrane / NarGHI complex / nitrate reductase complex / plasma membrane
- General Function
- Nitrate reductase activity
- Specific Function
- The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.
- Pfam Domain Function
- Nitrate_red_gam (PF02665)
- Transmembrane Regions
- 4-29 48-70 83-112 125-148 183-198
- Cellular Location
- Cell inner membrane
- Gene sequence
>lcl|BSEQ0017234|Respiratory nitrate reductase 1 gamma chain (narI) ATGCAATTCCTGAATATGTTCTTCTTTGATATCTACCCGTACATAGCCGGGGCGGTCTTC CTGATTGGTAGCTGGCTGCGTTATGACTACGGGCAGTACACCTGGCGCGCGGCGTCCAGC CAGATGCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTG GGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGG CTGCCGATTGAAGTGAAACAGAAAATGGCAATGTTTGCTGGTGGTGCCAGCGGCGTGCTG TGTCTGATTGGCGGCGTGCTGTTGCTGAAACGTCGTCTGTTCAGCCCACGCGTGCGTGCA ACCACTACCGGAGCGGATATCCTGATCCTGTCGCTGCTCGTTATCCAGTGCGCGCTGGGC CTGTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTT GGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGCGCTTCTCAACACCTCGATGGTGTG GCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTC TCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTG GTGCGCGCTCGTCACTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11350 UniProtKB Entry Name NARI_ECOLI GenBank Protein ID 146919 GenBank Gene ID M20147 - General References
- Sodergren EJ, DeMoss JA: narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes. J Bacteriol. 1988 Apr;170(4):1721-9. [Article]
- Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Sodergren EJ, Hsu PY, DeMoss JA: Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli. J Biol Chem. 1988 Nov 5;263(31):16156-62. [Article]
- Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. [Article]
- Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC: Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A. Nat Struct Biol. 2003 Sep;10(9):681-7. Epub 2003 Aug 10. [Article]