Exotoxin A
Details
- Name
- Exotoxin A
- Synonyms
- 2.4.2.36
- ETA
- NAD(+)--diphthamide ADP-ribosyltransferase
- PE
- Gene Name
- eta
- Organism
- Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
- Amino acid sequence
>lcl|BSEQ0011340|Exotoxin A MHLTPHWIPLVASLGLLAGGSFASAAEEAFDLWNECAKACVLDLKDGVRSSRMSVDPAIA DTNGQGVLHYSMVLEGGNDALKLAIDNALSITSDGLTIRLEGGVEPNKPVRYSYTRQARG SWSLNWLVPIGHEKPSNIKVFIHELNAGNQLSHMSPIYTIEMGDELLAKLARDATFFVRA HESNEMQPTLAISHAGVSVVMAQAQPRREKRWSEWASGKVLCLLDPLDGVYNYLAQQRCN LDDTWEGKIYRVLAGNPAKHDLDIKPTVISHRLHFPEGGSLAALTAHQACHLPLETFTRH RQPRGWEQLEQCGYPVQRLVALYLAARLSWNQVDQVIRNALASPGSGGDLGEAIREQPEQ ARLALTLAAAESERFVRQGTGNDEAGAASADVVSLTCPVAAGECAGPADSGDALLERNYP TGAEFLGDGGDISFSTRGTQNWTVERLLQAHRQLEERGYVFVGYHGTFLEAAQSIVFGGV RARSQDLDAIWRGFYIAGDPALAYGYAQDQEPDARGRIRNGALLRVYVPRSSLPGFYRTG LTLAAPEAAGEVERLIGHPLPLRLDAITGPEEEGGRLETILGWPLAERTVVIPSAIPTDP RNVGGDLDPSSIPDKEQAISALPDYASQPGKPPREDLK
- Number of residues
- 638
- Molecular Weight
- 69283.345
- Theoretical pI
- 5.29
- GO Classification
- FunctionsNAD+-diphthamide ADP-ribosyltransferase activity
- General Function
- Nad+-diphthamide adp-ribosyltransferase activity
- Specific Function
- An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0011341|Exotoxin A (eta) ATGCACCTGACACCCCATTGGATCCCCCTGGTCGCCAGCCTCGGCCTGCTCGCCGGCGGC TCGTTCGCGTCCGCCGCCGAGGAAGCCTTCGACCTCTGGAACGAATGCGCCAAGGCCTGC GTGCTCGACCTCAAGGACGGCGTGCGTTCCAGCCGCATGAGCGTCGACCCGGCCATCGCC GACACCAACGGCCAGGGCGTGCTGCACTACTCCATGGTCCTGGAGGGCGGCAACGACGCG CTCAAGCTGGCCATCGACAACGCCCTCAGCATCACCAGCGACGGCCTGACCATCCGCCTC GAAGGTGGCGTCGAGCCGAACAAGCCGGTGCGCTACAGCTACACGCGCCAGGCGCGCGGC AGTTGGTCGCTGAACTGGCTGGTGCCGATCGGCCACGAGAAGCCTTCGAACATCAAGGTG TTCATCCACGAACTGAACGCCGGTAACCAGCTCAGCCACATGTCGCCGATCTACACCATC GAGATGGGCGACGAGTTGCTGGCGAAGCTGGCGCGCGATGCCACCTTCTTCGTCAGGGCG CACGAGAGCAACGAGATGCAGCCGACGCTCGCCATCAGCCATGCCGGGGTCAGCGTGGTC ATGGCCCAGGCCCAGCCGCGCCGGGAAAAGCGCTGGAGCGAATGGGCCAGCGGCAAGGTG TTGTGCCTGCTCGACCCGCTGGACGGGGTCTACAACTACCTCGCCCAGCAGCGCTGCAAC CTCGACGATACCTGGGAAGGCAAGATCTACCGGGTGCTCGCCGGCAACCCGGCGAAGCAT GACCTGGACATCAAGCCCACGGTCATCAGTCATCGCCTGCATTTCCCCGAGGGCGGCAGC CTGGCCGCGCTGACCGCGCACCAGGCCTGCCACCTGCCGCTGGAGACCTTCACCCGTCAT CGCCAGCCGCGCGGCTGGGAACAACTGGAGCAGTGCGGCTATCCGGTGCAGCGGCTGGTC GCCCTCTACCTGGCGGCGCGGCTGTCGTGGAACCAGGTCGACCAGGTGATCCGCAACGCC CTGGCCAGCCCCGGCAGCGGCGGCGACCTGGGCGAAGCGATCCGCGAGCAGCCGGAGCAG GCCCGTCTGGCCCTGACCCTGGCCGCCGCCGAGAGCGAGCGCTTCGTCCGGCAGGGCACA GGCAACGACGAGGCCGGCGCGGCCAGCGCCGACGTGGTGAGCCTGACCTGCCCGGTCGCC GCCGGTGAATGCGCGGGCCCGGCGGACAGCGGCGACGCCCTGCTGGAGCGCAACTATCCC ACTGGCGCGGAGTTCCTCGGCGACGGCGGCGACATCAGCTTCAGCACCCGCGGCACGCAG AACTGGACGGTGGAGCGGCTGCTCCAGGCGCACCGCCAACTGGAGGAGCGCGGCTATGTG TTCGTCGGCTACCACGGCACCTTCCTCGAAGCGGCGCAAAGCATCGTCTTCGGCGGGGTG CGCGCGCGCAGCCAGGACCTCGACGCGATCTGGCGCGGTTTCTATATCGCCGGCGATCCG GCGCTGGCCTACGGCTACGCCCAGGACCAGGAACCCGACGCGCGCGGCCGGATCCGCAAC GGTGCCCTGCTGCGGGTCTATGTGCCGCGCTCGAGTCTGCCGGGCTTCTACCGCACCGGC CTGACCCTGGCCGCGCCGGAGGCGGCGGGCGAGGTCGAACGGCTGATCGGCCATCCGCTG CCGCTGCGCCTGGACGCCATCACCGGCCCCGAGGAGGAAGGCGGGCGCCTGGAGACCATT CTCGGCTGGCCGCTGGCCGAGCGCACCGTGGTGATTCCCTCGGCGATCCCCACCGACCCG CGCAACGTCGGCGGCGACCTCGACCCGTCCAGCATCCCCGACAAGGAACAGGCGATCAGC GCCCTGCCGGACTACGCCAGCCAGCCCGGCAAACCGCCGCGCGAGGACCTGAAGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11439 UniProtKB Entry Name TOXA_PSEAE GenBank Protein ID 151216 GenBank Gene ID K01397 - General References
- Gray GL, Smith DH, Baldridge JS, Harkins RN, Vasil ML, Chen EY, Heyneker HL: Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1984 May;81(9):2645-9. [Article]
- Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
- Carroll SF, Collier RJ: Active site of Pseudomonas aeruginosa exotoxin A. Glutamic acid 553 is photolabeled by NAD and shows functional homology with glutamic acid 148 of diphtheria toxin. J Biol Chem. 1987 Jun 25;262(18):8707-11. [Article]
- Chaudhary VK, Jinno Y, Gallo MG, FitzGerald D, Pastan I: Mutagenesis of Pseudomonas exotoxin in identification of sequences responsible for the animal toxicity. J Biol Chem. 1990 Sep 25;265(27):16306-10. [Article]
- Bourdenet S, Vacheron MJ, Guinand M, Michel G, Arminjon F: Biochemical and immunochemical studies of proteolytic fragments of exotoxin A from Pseudomonas aeruginosa. Eur J Biochem. 1990 Sep 11;192(2):379-85. [Article]
- Kounnas MZ, Morris RE, Thompson MR, FitzGerald DJ, Strickland DK, Saelinger CB: The alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A. J Biol Chem. 1992 Jun 25;267(18):12420-3. [Article]
- McKee ML, FitzGerald DJ: Reduction of furin-nicked Pseudomonas exotoxin A: an unfolding story. Biochemistry. 1999 Dec 14;38(50):16507-13. [Article]
- Jorgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Merrill AR: Cholix toxin, a novel ADP-ribosylating factor from Vibrio cholerae. J Biol Chem. 2008 Apr 18;283(16):10671-8. doi: 10.1074/jbc.M710008200. Epub 2008 Feb 14. [Article]
- Turgeon Z, Jorgensen R, Visschedyk D, Edwards PR, Legree S, McGregor C, Fieldhouse RJ, Mangroo D, Schapira M, Merrill AR: Newly discovered and characterized antivirulence compounds inhibit bacterial mono-ADP-ribosyltransferase toxins. Antimicrob Agents Chemother. 2011 Mar;55(3):983-91. doi: 10.1128/AAC.01164-10. Epub 2010 Dec 6. [Article]
- Li M, Dyda F, Benhar I, Pastan I, Davies DR: The crystal structure of Pseudomonas aeruginosa exotoxin domain III with nicotinamide and AMP: conformational differences with the intact exotoxin. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9308-12. [Article]
- Li M, Dyda F, Benhar I, Pastan I, Davies DR: Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation. Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6902-6. [Article]
- Jorgensen R, Wang Y, Visschedyk D, Merrill AR: The nature and character of the transition state for the ADP-ribosyltransferase reaction. EMBO Rep. 2008 Aug;9(8):802-9. doi: 10.1038/embor.2008.90. Epub 2008 Jun 27. [Article]