Urease subunit alpha

Details

Name
Urease subunit alpha
Synonyms
  • 3.5.1.5
  • Urea amidohydrolase subunit alpha
Gene Name
ureC
Organism
Enterobacter aerogenes
Amino acid sequence
>lcl|BSEQ0010092|Urease subunit alpha
MSNISRQAYADMFGPTVGDKVRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGMGQGQM
LAADCVDLVLTNALIVDHWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIGAATEVIA
AEGKIVTAGGIDTHIHWICPQQAEEALVSGVTTMVGGGTGPAAGTHATTCTPGPWYISRM
LQAADSLPVNIGLLGKGNVSQPDALREQVAAGVIGLKIHEDWGATPAAIDCALTVADEMD
IQVALHSDTLNESGFVEDTLAAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNP
TLPYTLNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRRETIAAEDVLHDLGAFSLTSSD
SQAMGRVGEVILRTWQVAHRMKVQRGALAEETGDNDNFRVKRYIAKYTINPALTHGIAHE
VGSIEVGKLADLVVWSPAFFGVKPATVIKGGMIAIAPMGDINASIPTPQPVHYRPMFGAL
GSARHHCRLTFLSQAAAANGVAERLNLRSAIAVVKGCRTVQKADMVHNSLQPNITVDAQT
YEVRVDGELITSEPADVLPMAQRYFLF
Number of residues
567
Molecular Weight
60304.12
Theoretical pI
5.03
GO Classification
Functions
nickel cation binding / urease activity
Processes
urea catabolic process
Components
cytoplasm
General Function
Urease activity
Specific Function
Not Available
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0000664|1704 bp
ATGAGTAATATTTCACGCCAGGCCTATGCCGATATGTTCGGCCCCACCGTCGGCGACAAG
GTGCGCCTGGCAGATACCGAGCTGTGGATCGAGGTGGAGGACGATTTGACCACCTACGGG
GAAGAGGTCAAATTCGGCGGCGGCAAAGTGATCCGCGACGGCATGGGCCAGGGACAGATG
CTGGCCGCCGACTGTGTCGACCTGGTGCTCACCAACGCGTTGATCGTCGATCACTGGGGG
ATCGTTAAGGCCGATATCGGCGTGAAGGACGGCCGGATCTTCGCCATCGGCAAAGCCGGC
AACCCCGACATCCAGCCCAACGTCACCATCCCCATCGGCGCTGCGACGGAAGTGATCGCC
GCCGAAGGAAAAATTGTCACCGCCGGCGGGATCGATACCCATATTCACTGGATCTGTCCG
CAGCAGGCGGAAGAGGCGCTGGTCTCTGGCGTGACCACCATGGTCGGCGGCGGCACCGGC
CCGGCCGCGGGCACCCATGCCACCACCTGCACCCCGGGCCCGTGGTATATCTCACGCATG
CTGCAGGCGGCCGACAGCCTGCCGGTCAATATCGGCCTGCTGGGCAAGGGAAACGTTTCT
CAGCCGGATGCCCTGCGCGAGCAGGTGGCGGCAGGCGTTATTGGCCTGAAGATCCATGAG
GACTGGGGCGCCACCCCGGCGGCGATCGACTGTGCGTTAACCGTCGCCGATGAAATGGAC
ATCCAGGTCGCCCTGCACAGCGACACCCTGAATGAATCCGGTTTTGTGGAAGACACCCTC
GCCGCCATCGGCGGGCGCACCATCCACACCTTCCATACCGAAGGGGCCGGCGGCGGCCAT
GCGCCGGACATCATCACCGCCTGCGCCCACCCGAACATTTTGCCGTCGTCCACCAACCCA
ACGCTGCCCTACACCCTCAACACCATCGATGAACATCTCGATATGCTGATGGTCTGCCAC
CATCTGGACCCGGACATCGCCGAGGACGTGGCCTTTGCCGAGTCGCGCATTCGCCGGGAA
ACCATCGCTGCGGAAGACGTGCTGCACGATCTCGGCGCCTTCTCGCTCACCTCCTCCGAT
TCGCAGGCCATGGGCCGCGTCGGGGAAGTGATTCTCCGCACCTGGCAGGTGGCGCATCGC
ATGAAGGTGCAGCGCGGAGCGCTGGCGGAGGAGACCGGGGATAACGACAACTTCCGCGTG
AAGCGCTACATCGCCAAATACACCATCAACCCGGCGCTGACCCACGGCATCGCACACGAA
GTCGGATCCATTGAGGTGGGTAAGCTGGCTGACCTCGTGGTCTGGTCACCAGCCTTCTTC
GGCGTGAAACCGGCCACCGTGATCAAAGGCGGCATGATCGCCATCGCGCCGATGGGCGAT
ATCAATGCCTCTATTCCGACCCCGCAGCCGGTGCACTACCGCCCGATGTTTGGCGCGCTG
GGCAGCGCCCGCCATCACTGCCGCCTCACCTTCCTGTCGCAGGCGGCGGCAGCCAATGGC
GTTGCCGAGCGGCTGAACCTGCGCAGCGCGATCGCCGTGGTGAAAGGCTGCCGTACGGTG
CAGAAAGCCGACATGGTGCACAACAGTCTGCAGCCTAACATCACCGTCGACGCCCAGACC
TATGAGGTGCGGGTGGATGGCGAACTTATCACCAGCGAGCCGGCAGACGTTCTGCCGATG
GCGCAACGATATTTTCTGTTTTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP18314
UniProtKB Entry NameURE1_ENTAE
GenBank Protein ID149337
GenBank Gene IDM36068
General References
  1. Mulrooney SB, Hausinger RP: Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation. J Bacteriol. 1990 Oct;172(10):5837-43. [Article]
  2. Lee MH, Mulrooney SB, Renner MJ, Markowicz Y, Hausinger RP: Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis. J Bacteriol. 1992 Jul;174(13):4324-30. [Article]
  3. Martin PR, Hausinger RP: Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease. J Biol Chem. 1992 Oct 5;267(28):20024-7. [Article]
  4. Park IS, Hausinger RP: Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis. Protein Sci. 1993 Jun;2(6):1034-41. [Article]
  5. Park IS, Carr MB, Hausinger RP: In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3233-7. [Article]
  6. Park IS, Hausinger RP: Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation. J Bacteriol. 1995 Apr;177(8):1947-51. [Article]
  7. Park IS, Hausinger RP: Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter. Science. 1995 Feb 24;267(5201):1156-8. [Article]
  8. Moncrief MB, Hausinger RP: Purification and activation properties of UreD-UreF-urease apoprotein complexes. J Bacteriol. 1996 Sep;178(18):5417-21. [Article]
  9. Soriano A, Hausinger RP: GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11140-4. [Article]
  10. Soriano A, Colpas GJ, Hausinger RP: UreE stimulation of GTP-dependent urease activation in the UreD-UreF-UreG-urease apoprotein complex. Biochemistry. 2000 Oct 10;39(40):12435-40. [Article]
  11. Mulrooney S, Zakharian T, Schaller RA, Hausinger RP: Dual effects of ionic strength on Klebsiella aerogenes urease: pH-dependent activation and inhibition. Arch Biochem Biophys. 2001 Oct 15;394(2):280-2. [Article]
  12. Chang Z, Kuchar J, Hausinger RP: Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease. J Biol Chem. 2004 Apr 9;279(15):15305-13. Epub 2004 Jan 28. [Article]
  13. Jabri E, Carr MB, Hausinger RP, Karplus PA: The crystal structure of urease from Klebsiella aerogenes. Science. 1995 May 19;268(5213):998-1004. [Article]
  14. Jabri E, Karplus PA: Structures of the Klebsiella aerogenes urease apoenzyme and two active-site mutants. Biochemistry. 1996 Aug 20;35(33):10616-26. [Article]
  15. Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP: Characterization of the mononickel metallocenter in H134A mutant urease. J Biol Chem. 1996 Aug 2;271(31):18632-7. [Article]
  16. Pearson MA, Michel LO, Hausinger RP, Karplus PA: Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease. Biochemistry. 1997 Jul 1;36(26):8164-72. [Article]
  17. Pearson MA, Schaller RA, Michel LO, Karplus PA, Hausinger RP: Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand. Biochemistry. 1998 Apr 28;37(17):6214-20. [Article]
  18. Yamaguchi K, Cosper NJ, Stalhandske C, Scott RA, Pearson MA, Karplus PA, Hausinger RP: Characterization of metal-substituted Klebsiella aerogenes urease. J Biol Inorg Chem. 1999 Aug;4(4):468-77. [Article]
  19. Pearson MA, Park IS, Schaller RA, Michel LO, Karplus PA, Hausinger RP: Kinetic and structural characterization of urease active site variants. Biochemistry. 2000 Jul 25;39(29):8575-84. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB00551Acetohydroxamic acidapprovedyesinhibitorDetails
DB05265EcabetinvestigationalyesinhibitorDetails