Deacetoxycephalosporin C synthase
Details
- Name
- Deacetoxycephalosporin C synthase
- Synonyms
- 1.14.20.1
- DAOCS
- Expandase
- Gene Name
- cefE
- Organism
- Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
- Amino acid sequence
>lcl|BSEQ0011313|Deacetoxycephalosporin C synthase MDTTVPTFSLAELQQGLHQDEFRRCLRDKGLFYLTDCGLTDTELKSAKDLVIDFFEHGSE AEKRAVTSPVPTMRRGFTGLESESTAQITNTGSYSDYSMCYSMGTADNLFPSGDFERIWT QYFDRQYTASRAVAREVLRATGTEPDGGVEAFLDCEPLLRFRYFPQVPEHRSAEEQPLRM APHYDLSMVTLIQQTPCANGFVSLQAEVGGAFTDLPYRPDAVLVFCGAIATLVTGGQVKA PRHHVAAPRRDQIAGSSRTSSVFFLRPNADFTFSVPLARECGFDVSLDGETATFQDWIGG NYVNIRRTSKA
- Number of residues
- 311
- Molecular Weight
- 34555.38
- Theoretical pI
- 4.94
- GO Classification
- Functionsdeacetoxycephalosporin-C synthase activity / iron ion binding / L-ascorbic acid bindingProcessesantibiotic biosynthetic process
- General Function
- L-ascorbic acid binding
- Specific Function
- Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0003631|936 bp ATGGACACGACGGTGCCCACCTTCAGCCTGGCCGAACTCCAGCAGGGCCTGCACCAGGAC GAGTTCCGCAGGTGTCTGAGGGACAAGGGCCTCTTCTATCTGACGGACTGCGGTCTGACC GACACCGAGCTGAAGTCGGCCAAGGACCTCGTCATCGACTTCTTCGAGCACGGCAGCGAG GCGGAGAAGCGCGCCGTCACCTCGCCCGTCCCCACCATGCGCCGCGGCTTCACCGGGCTG GAGTCGGAGAGCACCGCCCAGATCACCAATACCGGCAGCTACTCCGACTACTCGATGTGC TACTCGATGGGCACCGCGGACAACCTCTTCCCGTCCGGTGACTTCGAGCGGATCTGGACC CAGTACTTCGACCGCCAGTACACCGCCTCCCGCGCGGTCGCCCGGGAGGTCCTGCGGGCG ACCGGGACCGAGCCCGACGGCGGGGTCGAGGCCTTCCTCGACTGCGAGCCGCTGCTGCGG TTCCGCTACTTCCCGCAGGTCCCCGAGCACCGCAGCGCCGAGGAGCAGCCCCTGCGGATG GCGCCGCACTACGACCTGTCGATGGTCACCCTCATCCAGCAGACACCCTGCGCCAACGGC TTCGTCAGCCTCCAGGCCGAGGTCGGCGGCGCGTTCACGGACCTGCCCTACCGTCCGGAC GCCGTCCTCGTCTTCTGCGGCGCCATCGCGACCCTGGTGACCGGCGGCCAGGTCAAGGCC CCCCGGCACCATGTCGCGGCCCCCCGCAGGGACCAGATAGCGGGCAGCAGCCGCACCTCC AGTGTGTTCTTCCTCCGTCCCAACGCGGACTTCACCTTCTCCGTCCCGCTGGCGCGCGAG TGCGGCTTCGATGTCAGCCTGGACGGCGAGACCGCCACGTTCCAGGATTGGATCGGGGGC AACTACGTGAACATCCGCCGCACATCCAAGGCATAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P18548 UniProtKB Entry Name CEFE_STRC2 GenBank Protein ID 153205 GenBank Gene ID M32324 - General References
- Kovacevic S, Weigel BJ, Tobin MB, Ingolia TD, Miller JR: Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. J Bacteriol. 1989 Feb;171(2):754-60. [Article]
- Kovacevic S, Tobin MB, Miller JR: The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus. J Bacteriol. 1990 Jul;172(7):3952-8. [Article]