50S ribosomal protein L2

Details

Name
50S ribosomal protein L2
Synonyms
  • Hl4
  • Hmal2
Gene Name
rpl2
Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Amino acid sequence
>lcl|BSEQ0020802|50S ribosomal protein L2
MGRRIQGQRRGRGTSTFRAPSHRYKADLEHRKVEDGDVIAGTVVDIEHDPARSAPVAAVE
FEDGDRRLILAPEGVGVGDELQVGVSAEIAPGNTLPLAEIPEGVPVCNVESSPGDGGKFA
RASGVNAQLLTHDRNVAVVKLPSGEMKRLDPQCRATIGVVAGGGRTDKPFVKAGNKHHKM
KARGTKWPNVRGVAMNAVDHPFGGGGRQHPGKPKSISRNAPPGRKVGDIASKRTGRGGNE
Number of residues
240
Molecular Weight
25308.485
Theoretical pI
Not Available
GO Classification
Functions
rRNA binding / structural constituent of ribosome
Processes
translation
Components
large ribosomal subunit
General Function
Structural constituent of ribosome
Specific Function
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome (By similarity).
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0020803|50S ribosomal protein L2 (rpl2)
ATGGGACGACGAATCCAAGGACAACGGCGCGGCCGCGGGACCTCCACGTTCCGTGCTCCG
TCGCACCGTTACAAGGCTGATCTGGAGCACCGCAAGGTCGAGGACGGCGACGTCATCGCC
GGCACGGTCGTCGACATTGAGCACGACCCTGCCCGCTCGGCTCCGGTCGCTGCCGTCGAG
TTCGAAGACGGCGACCGACGACTTATCCTCGCGCCGGAAGGCGTCGGGGTAGGCGACGAG
CTACAGGTCGGCGTCAGCGCCGAGATCGCACCCGGGAACACGCTCCCGCTGGCCGAGATC
CCAGAGGGGGTCCCGGTGTGCAACGTCGAATCCAGCCCCGGTGACGGTGGGAAGTTCGCC
CGCGCGTCGGGTGTCAACGCCCAGCTGCTCACCCACGACCGCAACGTCGCGGTCGTCAAG
CTCCCCTCCGGGGAGATGAAGCGGCTTGACCCGCAGTGTCGTGCTACCATCGGCGTCGTC
GCCGGTGGCGGCCGAACTGACAAGCCGTTCGTGAAGGCTGGCAACAAGCATCACAAGATG
AAAGCGCGAGGGACGAAGTGGCCCAACGTCCGCGGTGTGGCGATGAACGCCGTCGACCAC
CCGTTCGGTGGTGGCGGACGCCAGCACCCCGGCAAGCCCAAGTCCATCTCGCGGAACGCC
CCGCCTGGCCGTAAGGTCGGGGACATCGCCTCGAAGCGAACAGGTCGAGGTGGCAACGAA
TGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP20276
UniProtKB Entry NameRL2_HALMA
General References
  1. Arndt E, Kromer W, Hatakeyama T: Organization and nucleotide sequence of a gene cluster coding for eight ribosomal proteins in the archaebacterium Halobacterium marismortui. J Biol Chem. 1990 Feb 25;265(6):3034-9. [Article]
  2. Baliga NS, Bonneau R, Facciotti MT, Pan M, Glusman G, Deutsch EW, Shannon P, Chiu Y, Weng RS, Gan RR, Hung P, Date SV, Marcotte E, Hood L, Ng WV: Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. Genome Res. 2004 Nov;14(11):2221-34. [Article]
  3. Uhlein M, Weglohner W, Urlaub H, Wittmann-Liebold B: Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies. Biochem J. 1998 Apr 15;331 ( Pt 2):423-30. [Article]
  4. Ban N, Nissen P, Hansen J, Capel M, Moore PB, Steitz TA: Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit. Nature. 1999 Aug 26;400(6747):841-7. [Article]
  5. Ban N, Nissen P, Hansen J, Moore PB, Steitz TA: The complete atomic structure of the large ribosomal subunit at 2.4 A resolution. Science. 2000 Aug 11;289(5481):905-20. [Article]
  6. Nissen P, Hansen J, Ban N, Moore PB, Steitz TA: The structural basis of ribosome activity in peptide bond synthesis. Science. 2000 Aug 11;289(5481):920-30. [Article]
  7. Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA: A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits. Nat Struct Biol. 2002 Mar;9(3):225-30. [Article]
  8. Klein DJ, Schmeing TM, Moore PB, Steitz TA: The kink-turn: a new RNA secondary structure motif. EMBO J. 2001 Aug 1;20(15):4214-21. [Article]
  9. Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA: The structures of four macrolide antibiotics bound to the large ribosomal subunit. Mol Cell. 2002 Jul;10(1):117-28. [Article]
  10. Hansen JL, Schmeing TM, Moore PB, Steitz TA: Structural insights into peptide bond formation. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11670-5. Epub 2002 Aug 16. [Article]
  11. Hansen JL, Moore PB, Steitz TA: Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit. J Mol Biol. 2003 Jul 25;330(5):1061-75. [Article]
  12. Schmeing TM, Moore PB, Steitz TA: Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit. RNA. 2003 Nov;9(11):1345-52. [Article]
  13. Gabdulkhakov A, Nikonov S, Garber M: Revisiting the Haloarcula marismortui 50S ribosomal subunit model. Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):997-1004. doi: 10.1107/S0907444913004745. Epub 2013 May 11. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB04865Omacetaxine mepesuccinateapproved, investigationalyesantagonistDetails