Cholesterol oxidase
Details
- Name
- Cholesterol oxidase
- Synonyms
- 1.1.3.6
- CHOD
- Cholesterol isomerase
- Gene Name
- choB
- Organism
- Brevibacterium sterolicum
- Amino acid sequence
>lcl|BSEQ0010856|Cholesterol oxidase MTDSRANRADATRGVASVSRRRFLAGAGLTAGAIALSSMSTSASAAPSRTLADGDRVPAL VIGSGYGGAVAALRLTQAGIPTQIVEMGRSWDTPGSDGKIFCGMLNPDKRSMWLADKTDQ PVSNFMGFGINKSIDRYVGVLDSERFSGIKVYQGRGVGGGSLVNGGMAVTPKRNYFEEIL PSVDSNEMYNKYFPRANTGLGVNNIDQAWFESTEWYKFARTGRKTAQRSGFTTAFVPNVY DFEYMKKEAAGQVTKSGLGGEVIYGNNAGKKSLDKTYLAQAAATGKLTITTLHRVTKVAP ATGSGYSVTMEQIDEQGNVVATKVVTADRVFFAAGSVGTSKLLVSMKAQGHLPNLSSQVG EGWGNNGNIMVGRANHMWDATGSKQATIPTMGIDNWADPTAPIFAEIAPLPAGLETYVSL YLAITKNPERARFQFNSGTGKVDLTWAQSQNQKGIDMAKKVFDKINQKEGTIYRTDLFGV YFKTWGDDFTYHPLGGVLLNKATDNFGRLPEYPGLYVVDGSLVPGNVGVNPFVTITRLAE RNMDKIISSDIQ
- Number of residues
- 552
- Molecular Weight
- 59357.765
- Theoretical pI
- 9.73
- GO Classification
- Functionscholesterol oxidase activity / flavin adenine dinucleotide binding / steroid delta-isomerase activityProcessescholesterol metabolic processComponentsextracellular region
- General Function
- Steroid delta-isomerase activity
- Specific Function
- Catalyzes the oxidation and isomerization of cholesterol to cholestenone (4-cholesten-3-one), which is an initial step in the cholesterol degradation process.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Gene sequence
>lcl|BSEQ0002448|1659 bp ATGACCGATAGCCGGGCGAACAGAGCCGATGCGACTCGGGGGGTTGCATCCGTCTCACGC CGTCGATTCCTTGCGGGCGCAGGTCTGACCGCGGGAGCCATCGCGCTCTCGTCGATGTCG ACCTCCGCCTCCGCAGCCCCCAGCCGCACCCTCGCCGACGGCGACCGCGTCCCTGCCCTC GTCATCGGCAGTGGATACGGCGGTGCCGTCGCCGCGCTGCGGCTGACGCAGGCCGGTATC CCCACGCAGATCGTCGAGATGGGCCGCAGCTGGGACACCCCGGGCTCCGACGGCAAGATC TTCTGCGGGATGCTCAACCCCGACAAGCGCTCGATGTGGTTGGCCGACAAGACCGATCAG CCGGTCAGCAACTTCATGGGCTTCGGCATCAACAAGAGCATCGACCGGTACGTCGGCGTC CTCGACTCCGAGCGGTTCTCCGGCATCAAGGTCTACCAGGGCCGCGGCGTCGGCGGCGGC TCGCTCGTCAACGGCGGTATGGCAGTCACCCCGAAGCGCAACTACTTCGAGGAGATCCTG CCGTCGGTCGACTCGAACGAGATGTACAACAAGTACTTCCCGCGCGCCAACACCGGTCTG GGTGTCAACAACATCGACCAGGCGTGGTTCGAGTCCACCGAGTGGTACAAGTTCGCCCGC ACCGGCCGCAAGACCGCCCAACGTTCGGGTTTCACAACCGCTTTCGTGCCCAACGTGTAC GACTTCGAGTACATGAAGAAGGAGGCTGCCGGCCAGGTCACCAAGTCGGGCCTCGGCGGT GAGGTCATCTACGGCAACAACGCCGGCAAGAAGTCGCTCGACAAGACCTACCTCGCGCAG GCCGCGGCCACCGGGAAGCTGACGATCACGACTTTGCACCGCGTCACCAAGGTCGCGCCG GCCACCGGCAGCGGCTACAGCGTGACGATGGAACAGATCGACGAGCAGGGCAACGTCGTC GCCACCAAGGTCGTCACCGCCGATCGGGTGTTCTTCGCGGCCGGCAGCGTCGGCACCAGC AAGCTCCTGGTCTCGATGAAGGCGCAGGGCCACCTGCCGAACCTGTCGTCGCAGGTCGGC GAGGGCTGGGGCAACAACGGCAACATCATGGTGGGCCGCGCGAACCACATGTGGGACGCC ACCGGATCCAAGCAGGCCACCATCCCGACGATGGGAATCGACAACTGGGCCGACCCGACG GCACCGATCTTCGCGGAGATCGCCCCGCTGCCGGCCGGGCTCGAGACCTACGTCAGCCTG TACCTGGCCATCACGAAGAACCCCGAACGTGCTCGCTTCCAGTTCAATTCGGGCACCGGC AAGGTCGATCTCACCTGGGCTCAGTCGCAGAACCAGAAGGGCATCGACATGGCCAAGAAG GTGTTCGACAAGATCAACCAGAAGGAGGGCACCATCTACCGCACCGATCTGTTCGGTGTC TACTTCAAGACCTGGGGCGACGACTTCACGTACCACCCGCTGGGCGGCGTGCTGCTGAAC AAGGCGACCGACAACTTTGGCCGCTTGCCCGAGTACCCCGGCCTGTACGTGGTGGACGGC TCGCTCGTCCCCGGCAATGTGGGCGTCAACCCGTTCGTCACGATCACCCGGCTCGCCGAG CGCAACATGGACAAGATCATCTCGTCCGACATCCAGTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P22637 UniProtKB Entry Name CHOD_BREST GenBank Protein ID 216385 GenBank Gene ID D00712 - General References
- Ohta T, Fujishiro K, Yamaguchi K, Tamura Y, Aisaka K, Uwajima T, Hasegawa M: Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: comparison with choA of streptomyces sp. SA-COO. Gene. 1991 Jul 15;103(1):93-6. [Article]
- Fujishiro K, Ohta T, Hasegawa M, Yamaguchi K, Mizukami T, Uwajima T, Ota T: Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis. Biochem Biophys Res Commun. 1990 Oct 30;172(2):721-7. [Article]
- Vrielink A, Lloyd LF, Blow DM: Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8 A resolution. J Mol Biol. 1991 Jun 5;219(3):533-54. [Article]