Aliphatic amidase expression-regulating protein

Details

Name
Aliphatic amidase expression-regulating protein
Synonyms
Not Available
Gene Name
amiC
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Amino acid sequence
>lcl|BSEQ0016430|Aliphatic amidase expression-regulating protein
MGSHQERPLIGLLFSETGVTADIERSQRYGALLAVEQLNREGGVGGRPIETLSQDPGGDP
DRYRLCAEDFIRNRGVRFLVGCYMSHTRKAVMPVVERADALLCYPTPYEGFEYSPNIVYG
GPAPNQNSAPLAAYLIRHYGERVVFIGSDYIYPRESNHVMRHLYRQHGGTVLEEIYIPLY
PSDDDVQRAVERIYQARADVVFSTVVGTGTAELYRAIARRYGDGRRPPIASLTTSEAEVA
KMESDVAEGQVVVAPYFSSIDTAASRAFVQACHGFFPENATITAWAEAAYWQTLLLGRAA
QAAGSWRVEDVQRHLYDICIDAPQGPVRVERQNNHSRLSSRIAEIDARGVFQVRWQSPEP
IRPDPYVVVHNLDDWSASMGGGALP
Number of residues
385
Molecular Weight
42806.69
Theoretical pI
5.82
GO Classification
Functions
amide binding / kinase activity
Processes
amino acid transport / negative regulation of hydrolase activity / regulation of cellular amide catabolic process
General Function
Kinase activity
Specific Function
Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity.
Pfam Domain Function
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016431|Aliphatic amidase expression-regulating protein (amiC)
ATGGGATCGCACCAGGAGCGGCCGCTGATCGGCCTGCTGTTCTCCGAAACCGGCGTCACC
GCCGATATCGAGCGCTCGCAGCGCTATGGCGCATTGCTTGCGGTCGAGCAACTGAACCGC
GAGGGCGGCGTCGGCGGTCGCCCGATCGAAACGCTGTCCCAGGACCCCGGCGGCGATCCG
GACCGCTATCGGCTGTGCGCCGAGGACTTCATTCGCAACCGGGGGGTACGGTTCCTCGTG
GGCTGCTACATGTCGCACACGCGCAAGGCGGTGATGCCGGTGGTCGAGCGCGCCGACGCG
CTGCTCTGCTACCCGACTCCCTACGAGGGCTTCGAGTATTCGCCGAACATCGTCTACGGC
GGTCCGGCGCCGAACCAGAACAGTGCGCCGCTGGCGGCGTACCTGATTCGCCACTACGGC
GAGCGGGTGGTGTTCATCGGCTCGGACTACATCTATCCGCGGGAAAGCAACCATGTGATG
CGCCACCTGTACCGCCAGCACGGCGGCACGGTGCTCGAGGAAATCTACATTCCGCTGTAT
CCCTCCGACGACGACGTGCAGCGCGCCGTCGAGCGCATCTACCAGGCGCGCGCCGACGTG
GTCTTCTCCACCGTGGTGGGCACCGGCACCGCCGAGCTGTATCGCGCCATCGCCCGTCGC
TACGGCGATGGCAGGCGGCCGCCGATCGCCAGCCTGACCACCAGCGAGGCGGAGGTGGCG
AAGATGGAGAGCGACGTGGCGGAGGGGCAGGTGGTGGTCGCGCCGTACTTCTCCAGCATC
GATACGGCCGCCAGCCGCGCCTTCGTCCAGGCCTGCCATGGCTTCTTCCCGGAAAACGCG
ACCATCACCGCCTGGGCCGAGGCGGCCTACTGGCAGACCTTGCTGCTTGGCCGCGCCGCG
CAGGCCGCAGGCAGTTGGCGAGTGGAAGACGTGCAGCGGCACCTGTACGACATTTGCATC
GACGCGCCCCAGGGGCCGGTCCGGGTGGAGCGCCAGAACAACCACAGCCGCCTGTCTTCG
CGCATCGCGGAAATCGATGCGCGCGGCGTGTTCCAGGTCCGCTGGCAGTCGCCCGAACCG
ATTCGCCCCGATCCTTATGTCGTCGTGCATAACCTCGACGACTGGTCCGCCAGCATGGGC
GGGGGAGCGCTCCCATGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP27017
UniProtKB Entry NameAMIC_PSEAE
GenBank Protein ID45271
GenBank Gene IDX13776
General References
  1. Wilson S, Drew R: Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product. J Bacteriol. 1991 Aug;173(16):4914-21. [Article]
  2. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
  3. Wilson SA, Chayen NE, Hemmings AM, Drew RE, Pearl LH: Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa. J Mol Biol. 1991 Dec 20;222(4):869-71. [Article]
  4. Pearl L, O'Hara B, Drew R, Wilson S: Crystal structure of AmiC: the controller of transcription antitermination in the amidase operon of Pseudomonas aeruginosa. EMBO J. 1994 Dec 15;13(24):5810-7. [Article]
  5. O'Hara BP, Norman RA, Wan PT, Roe SM, Barrett TE, Drew RE, Pearl LH: Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex. EMBO J. 1999 Oct 1;18(19):5175-86. [Article]
  6. O'Hara BP, Wilson SA, Lee AW, Roe SM, Siligardi G, Drew RE, Pearl LH: Structural adaptation to selective pressure for altered ligand specificity in the Pseudomonas aeruginosa amide receptor, amiC. Protein Eng. 2000 Feb;13(2):129-32. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB02121ButyramideexperimentalunknownDetails
DB02736AcetamideexperimentalunknownDetails