Queuine tRNA-ribosyltransferase

Details

Name
Queuine tRNA-ribosyltransferase
Synonyms
  • 2.4.2.29
  • Guanine insertion enzyme
  • tRNA-guanine transglycosylase
Gene Name
tgt
Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Amino acid sequence
>lcl|BSEQ0002789|Queuine tRNA-ribosyltransferase
MVEATAQETDRPRFSFSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVR
ATGADIILGNTYHLMLRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEE
GVTFKSHLDGSRHMLSPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAK
RSRDAFDSRKEQAENAALFGIQQGSVFENLRQQSADALAEIGFDGYAVGGLAVGEGQDEM
FRVLDFSVPMLPDDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPI
NIRNARFSEDLTPLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQK
IRDSISEGRFSQFAQDFRARYFARNS
Number of residues
386
Molecular Weight
42842.235
Theoretical pI
6.85
GO Classification
Functions
metal ion binding / queuine tRNA-ribosyltransferase activity
Processes
queuosine biosynthetic process / tRNA modification
General Function
Queuine trna-ribosyltransferase activity
Specific Function
Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016412|Queuine tRNA-ribosyltransferase (tgt)
GTGGTAGAAGCAACAGCGCAAGAGACCGATCGTCCGCGTTTTTCTTTTTCAATCGCGGCA
AGGGAAGGAAAAGCCCGCACCGGCACTATCGAAATGAAGCGGGGCGTTATCCGAACCCCT
GCCTTTATGCCGGTTGGAACGGCAGCTACCGTAAAGGCTTTGAAGCCGGAAACAGTTCGG
GCAACTGGCGCTGATATTATCTTGGGGAATACCTATCATTTGATGCTTCGTCCGGGTGCC
GAACGGATAGCTAAGCTGGGCGGATTACATTCTTTTATGGGGTGGGATCGGCCTATTTTG
ACGGATAGCGGCGGGTATCAGGTGATGAGCCTGTCTTCTTTGACGAAGCAGAGCGAAGAG
GGCGTTACCTTTAAAAGTCATCTTGACGGTTCCCGCCATATGCTGTCGCCGGAACGTTCT
ATCGAAATCCAGCATTTACTAGGCAGTGATATCGTAATGGCCTTTGACGAATGCACGCCT
TATCCGGCAACGCCTTCGCGCGCGGCCTCGTCAATGGAACGCTCGATGCGGTGGGCGAAA
AGATCACGGGATGCCTTTGATAGCCGAAAAGAACAGGCAGAAAATGCGGCTTTGTTCGGA
ATTCAACAAGGTTCTGTTTTTGAAAATCTGCGGCAACAATCGGCGGATGCTCTGGCTGAA
ATCGGCTTTGATGGCTATGCTGTTGGGGGATTGGCTGTGGGTGAAGGACAGGATGAAATG
TTCCGCGTCCTTGATTTTTCTGTGCCGATGCTGCCCGATGACAAACCTCATTATCTTATG
GGCGTTGGTAAGCCTGATGATATCGTTGGGGCGGTTGAACGCGGCATTGATATGTTCGAT
TGCGTCTTGCCGACACGTTCCGGTCGGAATGGGCAAGCCTTTACATGGGATGGGCCTATC
AATATCAGAAATGCTCGTTTTTCAGAAGATTTGACGCCGTTGGATAGTGAATGTCATTGT
GCCGTTTGCCAGAAATGGAGCCGCGCCTATATCCATCATTTAATTCGGGCGGGTGAGATC
TTGGGCGCTATGCTGATGACAGAGCATAATATCGCCTTTTACCAACAGCTTATGCAAAAA
ATACGGGACTCTATTTCGGAGGGGCGTTTTTCGCAATTTGCTCAGGATTTCAGAGCGCGC
TATTTCGCACGGAATAGCTAG
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP28720
UniProtKB Entry NameTGT_ZYMMO
GenBank Protein ID498141
GenBank Gene IDL33777
General References
  1. Reuter K, Ficner R: Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzyme. J Bacteriol. 1995 Sep;177(18):5284-8. [Article]
  2. Seo JS, Chong H, Park HS, Yoon KO, Jung C, Kim JJ, Hong JH, Kim H, Kim JH, Kil JI, Park CJ, Oh HM, Lee JS, Jin SJ, Um HW, Lee HJ, Oh SJ, Kim JY, Kang HL, Lee SY, Lee KJ, Kang HS: The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4. Nat Biotechnol. 2005 Jan;23(1):63-8. Epub 2004 Dec 12. [Article]
  3. Shark KB, Conway T: Cloning and molecular characterization of the DNA ligase gene (lig) from Zymomonas mobilis. FEMS Microbiol Lett. 1992 Sep 1;75(1):19-26. [Article]
  4. Romier C, Reuter K, Suck D, Ficner R: Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange. EMBO J. 1996 Jun 3;15(11):2850-7. [Article]
  5. Gradler U, Ficner R, Garcia GA, Stubbs MT, Klebe G, Reuter K: Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity. FEBS Lett. 1999 Jul 2;454(1-2):142-6. [Article]
  6. Gradler U, Gerber HD, Goodenough-Lashua DM, Garcia GA, Ficner R, Reuter K, Stubbs MT, Klebe G: A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase. J Mol Biol. 2001 Feb 23;306(3):455-67. [Article]
  7. Meyer EA, Brenk R, Castellano RK, Furler M, Klebe G, Diederich F: De novo design, synthesis, and in vitro evaluation of inhibitors for prokaryotic tRNA-guanine transglycosylase: a dramatic sulfur effect on binding affinity. Chembiochem. 2002 Mar 1;3(2-3):250-3. [Article]
  8. Brenk R, Naerum L, Gradler U, Gerber HD, Garcia GA, Reuter K, Stubbs MT, Klebe G: Virtual screening for submicromolar leads of tRNA-guanine transglycosylase based on a new unexpected binding mode detected by crystal structure analysis. J Med Chem. 2003 Mar 27;46(7):1133-43. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB018252-amino-8-methyl-4(1H)-quinazolinoneexperimentalunknownDetails
DB02041IsoluminolexperimentalunknownDetails
DB024412-Butyl-5,6-Dihydro-1h-Imidazo[4,5-D]Pyridazine-4,7-DioneexperimentalunknownDetails
DB025992,6-Diamino-8-Propylsulfanylmethyl-3h-Quinazoline-4-OneexperimentalunknownDetails
DB030747-cyano-7-deazaguanineexperimentalunknownDetails
DB033047-Deaza-7-Aminomethyl-GuanineexperimentalunknownDetails
DB035052,6-diaminoquinazolin-4-olexperimentalunknownDetails
DB037802-Aminoquinazolin-4(3h)-OneexperimentalunknownDetails
DB040042,6-Diamino-8-(2-Dimethylaminoethylsulfanylmethyl)-3h-Quinazolin-4-OneexperimentalunknownDetails
DB041693,5-DiaminophthalhydrazideexperimentalunknownDetails
DB042392-Amino-6-Aminomethyl-8-Phenylsulfanylmethyl-3h-Quinazolin-4-OneexperimentalunknownDetails
DB045432,6-Diamino-8-(1h-Imidazol-2-Ylsulfanylmethyl)-3h-Quinazoline-4-OneexperimentalunknownDetails
DB070126-AMINO-3,7-DIHYDRO-IMIDAZO[4,5-G]QUINAZOLIN-8-ONEexperimentalunknownDetails
DB074522,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-oneexperimentalunknownDetails
DB07481tert-butyl [(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]carbamateexperimentalunknownDetails
DB075646-amino-2-[(2-morpholin-4-ylethyl)amino]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-oneexperimentalunknownDetails
DB077046-AMINO-4-[2-(4-METHOXYPHENYL)ETHYL]-1,7-DIHYDRO-8H-IMIDAZO[4,5-G]QUINAZOLIN-8-ONEexperimentalunknownDetails
DB082676-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-oneexperimentalunknownDetails
DB082686-AMINO-4-[2-(4-METHYLPHENYL)ETHYL]-1,7-DIHYDRO-8H-IMIDAZO[4,5-G]QUINAZOLIN-8-ONEexperimentalunknownDetails
DB085116-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-oneexperimentalunknownDetails
DB085126-amino-2-[(1-naphthylmethyl)amino]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-oneexperimentalunknownDetails
DB085146-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-oneexperimentalunknownDetails
DB14732Queuineexperimental, nutraceuticalunknownsubstrateDetails