Queuine tRNA-ribosyltransferase
Details
- Name
- Queuine tRNA-ribosyltransferase
- Synonyms
- 2.4.2.29
- Guanine insertion enzyme
- tRNA-guanine transglycosylase
- Gene Name
- tgt
- Organism
- Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
- Amino acid sequence
>lcl|BSEQ0002789|Queuine tRNA-ribosyltransferase MVEATAQETDRPRFSFSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVR ATGADIILGNTYHLMLRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEE GVTFKSHLDGSRHMLSPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAK RSRDAFDSRKEQAENAALFGIQQGSVFENLRQQSADALAEIGFDGYAVGGLAVGEGQDEM FRVLDFSVPMLPDDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPI NIRNARFSEDLTPLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQK IRDSISEGRFSQFAQDFRARYFARNS
- Number of residues
- 386
- Molecular Weight
- 42842.235
- Theoretical pI
- 6.85
- GO Classification
- Functionsmetal ion binding / queuine tRNA-ribosyltransferase activityProcessesqueuosine biosynthetic process / tRNA modification
- General Function
- Queuine trna-ribosyltransferase activity
- Specific Function
- Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
- Pfam Domain Function
- TGT (PF01702)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016412|Queuine tRNA-ribosyltransferase (tgt) GTGGTAGAAGCAACAGCGCAAGAGACCGATCGTCCGCGTTTTTCTTTTTCAATCGCGGCA AGGGAAGGAAAAGCCCGCACCGGCACTATCGAAATGAAGCGGGGCGTTATCCGAACCCCT GCCTTTATGCCGGTTGGAACGGCAGCTACCGTAAAGGCTTTGAAGCCGGAAACAGTTCGG GCAACTGGCGCTGATATTATCTTGGGGAATACCTATCATTTGATGCTTCGTCCGGGTGCC GAACGGATAGCTAAGCTGGGCGGATTACATTCTTTTATGGGGTGGGATCGGCCTATTTTG ACGGATAGCGGCGGGTATCAGGTGATGAGCCTGTCTTCTTTGACGAAGCAGAGCGAAGAG GGCGTTACCTTTAAAAGTCATCTTGACGGTTCCCGCCATATGCTGTCGCCGGAACGTTCT ATCGAAATCCAGCATTTACTAGGCAGTGATATCGTAATGGCCTTTGACGAATGCACGCCT TATCCGGCAACGCCTTCGCGCGCGGCCTCGTCAATGGAACGCTCGATGCGGTGGGCGAAA AGATCACGGGATGCCTTTGATAGCCGAAAAGAACAGGCAGAAAATGCGGCTTTGTTCGGA ATTCAACAAGGTTCTGTTTTTGAAAATCTGCGGCAACAATCGGCGGATGCTCTGGCTGAA ATCGGCTTTGATGGCTATGCTGTTGGGGGATTGGCTGTGGGTGAAGGACAGGATGAAATG TTCCGCGTCCTTGATTTTTCTGTGCCGATGCTGCCCGATGACAAACCTCATTATCTTATG GGCGTTGGTAAGCCTGATGATATCGTTGGGGCGGTTGAACGCGGCATTGATATGTTCGAT TGCGTCTTGCCGACACGTTCCGGTCGGAATGGGCAAGCCTTTACATGGGATGGGCCTATC AATATCAGAAATGCTCGTTTTTCAGAAGATTTGACGCCGTTGGATAGTGAATGTCATTGT GCCGTTTGCCAGAAATGGAGCCGCGCCTATATCCATCATTTAATTCGGGCGGGTGAGATC TTGGGCGCTATGCTGATGACAGAGCATAATATCGCCTTTTACCAACAGCTTATGCAAAAA ATACGGGACTCTATTTCGGAGGGGCGTTTTTCGCAATTTGCTCAGGATTTCAGAGCGCGC TATTTCGCACGGAATAGCTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P28720 UniProtKB Entry Name TGT_ZYMMO GenBank Protein ID 498141 GenBank Gene ID L33777 - General References
- Reuter K, Ficner R: Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzyme. J Bacteriol. 1995 Sep;177(18):5284-8. [Article]
- Seo JS, Chong H, Park HS, Yoon KO, Jung C, Kim JJ, Hong JH, Kim H, Kim JH, Kil JI, Park CJ, Oh HM, Lee JS, Jin SJ, Um HW, Lee HJ, Oh SJ, Kim JY, Kang HL, Lee SY, Lee KJ, Kang HS: The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4. Nat Biotechnol. 2005 Jan;23(1):63-8. Epub 2004 Dec 12. [Article]
- Shark KB, Conway T: Cloning and molecular characterization of the DNA ligase gene (lig) from Zymomonas mobilis. FEMS Microbiol Lett. 1992 Sep 1;75(1):19-26. [Article]
- Romier C, Reuter K, Suck D, Ficner R: Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange. EMBO J. 1996 Jun 3;15(11):2850-7. [Article]
- Gradler U, Ficner R, Garcia GA, Stubbs MT, Klebe G, Reuter K: Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity. FEBS Lett. 1999 Jul 2;454(1-2):142-6. [Article]
- Gradler U, Gerber HD, Goodenough-Lashua DM, Garcia GA, Ficner R, Reuter K, Stubbs MT, Klebe G: A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase. J Mol Biol. 2001 Feb 23;306(3):455-67. [Article]
- Meyer EA, Brenk R, Castellano RK, Furler M, Klebe G, Diederich F: De novo design, synthesis, and in vitro evaluation of inhibitors for prokaryotic tRNA-guanine transglycosylase: a dramatic sulfur effect on binding affinity. Chembiochem. 2002 Mar 1;3(2-3):250-3. [Article]
- Brenk R, Naerum L, Gradler U, Gerber HD, Garcia GA, Reuter K, Stubbs MT, Klebe G: Virtual screening for submicromolar leads of tRNA-guanine transglycosylase based on a new unexpected binding mode detected by crystal structure analysis. J Med Chem. 2003 Mar 27;46(7):1133-43. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB01825 2-amino-8-methyl-4(1H)-quinazolinone experimental unknown Details DB02041 Isoluminol experimental unknown Details DB02441 2-Butyl-5,6-Dihydro-1h-Imidazo[4,5-D]Pyridazine-4,7-Dione experimental unknown Details DB02599 2,6-Diamino-8-Propylsulfanylmethyl-3h-Quinazoline-4-One experimental unknown Details DB03074 7-cyano-7-deazaguanine experimental unknown Details DB03304 7-Deaza-7-Aminomethyl-Guanine experimental unknown Details DB03505 2,6-diaminoquinazolin-4-ol experimental unknown Details DB03780 2-Aminoquinazolin-4(3h)-One experimental unknown Details DB04004 2,6-Diamino-8-(2-Dimethylaminoethylsulfanylmethyl)-3h-Quinazolin-4-One experimental unknown Details DB04169 3,5-Diaminophthalhydrazide experimental unknown Details DB04239 2-Amino-6-Aminomethyl-8-Phenylsulfanylmethyl-3h-Quinazolin-4-One experimental unknown Details DB04543 2,6-Diamino-8-(1h-Imidazol-2-Ylsulfanylmethyl)-3h-Quinazoline-4-One experimental unknown Details DB07012 6-AMINO-3,7-DIHYDRO-IMIDAZO[4,5-G]QUINAZOLIN-8-ONE experimental unknown Details DB07452 2,6-diamino-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one experimental unknown Details DB07481 tert-butyl [(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]carbamate experimental unknown Details DB07564 6-amino-2-[(2-morpholin-4-ylethyl)amino]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one experimental unknown Details DB07704 6-AMINO-4-[2-(4-METHOXYPHENYL)ETHYL]-1,7-DIHYDRO-8H-IMIDAZO[4,5-G]QUINAZOLIN-8-ONE experimental unknown Details DB08267 6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one experimental unknown Details DB08268 6-AMINO-4-[2-(4-METHYLPHENYL)ETHYL]-1,7-DIHYDRO-8H-IMIDAZO[4,5-G]QUINAZOLIN-8-ONE experimental unknown Details DB08511 6-amino-2-methyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one experimental unknown Details DB08512 6-amino-2-[(1-naphthylmethyl)amino]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one experimental unknown Details DB08514 6-amino-2-[(thiophen-2-ylmethyl)amino]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one experimental unknown Details DB14732 Queuine experimental, nutraceutical unknown substrate Details