Protoporphyrinogen oxidase
Details
- Name
- Protoporphyrinogen oxidase
- Synonyms
- 1.3.3.4
- hemG
- PPO
- Gene Name
- hemY
- Organism
- Bacillus subtilis (strain 168)
- Amino acid sequence
>lcl|BSEQ0012654|Protoporphyrinogen oxidase MSDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASPRVGGKIQTVKKDGYII ERGPDSFLERKKSAPQLVKDLGLEHLLVNNATGQSYVLVNRTLHPMPKGAVMGIPTKIAP FVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRVGDEVVENLIEPLLSGIYAGD IDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQLTAKKQGQFQTLSTGLQTLVEE IEKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTLDADSVIVTAPHKAAAGMLSELPAI SHLKNMHSTSVANVALGFPEGSVQMEHEGTGFVISRNSDFAITACTWTNKKWPHAAPEGK TLLRAYVGKAGDESIVDLSDNDIINIVLEDLKKVMNINGEPEMTCVTRWHESMPQYHVGH KQRIKELREALASAYPGVYMTGASFEGVGIPDCIDQGKAAVSDALTYLFS
- Number of residues
- 470
- Molecular Weight
- 51202.545
- Theoretical pI
- 8.3
- GO Classification
- Functionsoxygen-dependent protoporphyrinogen oxidase activityProcessesprotoporphyrinogen IX biosynthetic processComponentscytoplasm
- General Function
- Oxygen-dependent protoporphyrinogen oxidase activity
- Specific Function
- Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Also oxidizes the pathway intermediate coproporphyrinogen-III.
- Pfam Domain Function
- Amino_oxidase (PF01593)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0012655|Protoporphyrinogen oxidase (hemY) ATGAGTGACGGCAAAAAACATGTAGTCATCATCGGCGGCGGCATTACCGGTTTAGCCGCC GCCTTCTATATGGAAAAAGAAATCAAAGAAAAGAATCTGCCGCTTGAGCTGACACTTGTT GAGGCAAGTCCGAGAGTCGGCGGGAAAATCCAGACTGTCAAGAAAGACGGCTATATCATC GAAAGAGGGCCAGACTCATTTCTGGAACGAAAGAAAAGCGCCCCGCAGCTTGTTAAAGAC TTAGGTCTTGAGCATTTGCTTGTCAACAATGCGACCGGGCAATCCTATGTGCTTGTAAAC CGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCG TTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATC CTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTC GGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCAGGGATCTACGCAGGCGAC ATTGACAAGCTCAGCCTGATGTCGACATTTCCGCAATTTTATCAGACGGAACAAAAGCAT AGAAGCCTGATTCTCGGCATGAAAAAAACAAGGCCTCAAGGCTCAGGCCAGCAGCTGACG GCAAAAAAACAAGGGCAGTTCCAAACTCTGTCAACCGGTTTGCAGACCCTTGTAGAAGAG ATCGAAAAGCAGTTAAAGCTGACGAAGGTGTATAAAGGCACAAAAGTGACCAAACTTAGC CATAGCGGCTCTTGCTATTCGCTCGAACTGGATAACGGCGTCACACTTGATGCTGATTCA GTAATTGTGACTGCTCCGCATAAAGCGGCTGCGGGAATGCTTTCTGAGCTTCCTGCCATT TCTCATTTGAAAAATATGCACTCCACATCCGTGGCAAACGTCGCTTTAGGTTTCCCTGAA GGCTCCGTCCAAATGGAGCATGAGGGCACGGGTTTTGTCATTTCAAGAAACAGTGACTTT GCGATCACAGCCTGTACGTGGACGAATAAAAAATGGCCGCACGCAGCGCCGGAAGGCAAA ACGCTGCTTCGGGCATATGTCGGAAAAGCCGGAGACGAATCCATTGTCGATCTATCAGAT AATGACATTATCAACATTGTGTTAGAAGACTTAAAGAAAGTCATGAACATAAACGGCGAG CCGGAAATGACATGTGTAACCCGATGGCATGAAAGCATGCCGCAGTACCATGTCGGCCAT AAGCAGCGTATCAAGGAGCTGCGTGAAGCACTTGCATCTGCGTATCCGGGTGTTTATATG ACAGGCGCTTCTTTCGAAGGTGTCGGCATTCCCGACTGCATTGATCAAGGAAAAGCTGCC GTGTCTGACGCGCTTACCTATTTATTCAGCTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P32397 UniProtKB Entry Name PPOX_BACSU GenBank Protein ID 143042 GenBank Gene ID M97208 - General References
- Hansson M, Hederstedt L: Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes. J Bacteriol. 1992 Dec;174(24):8081-93. [Article]
- Noback MA, Holsappel S, Kiewiet R, Terpstra P, Wambutt R, Wedler H, Venema G, Bron S: The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene. Microbiology. 1998 Apr;144 ( Pt 4):859-75. [Article]
- Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
- Dailey TA, Meissner P, Dailey HA: Expression of a cloned protoporphyrinogen oxidase. J Biol Chem. 1994 Jan 14;269(2):813-5. [Article]
- Sun L, Wen X, Tan Y, Li H, Yang X, Zhao Y, Wang B, Cao Q, Niu C, Xi Z: Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase. Amino Acids. 2009 Sep;37(3):523-30. doi: 10.1007/s00726-009-0256-5. Epub 2009 Mar 6. [Article]
- Qin X, Sun L, Wen X, Yang X, Tan Y, Jin H, Cao Q, Zhou W, Xi Z, Shen Y: Structural insight into unique properties of protoporphyrinogen oxidase from Bacillus subtilis. J Struct Biol. 2010 Apr;170(1):76-82. doi: 10.1016/j.jsb.2009.11.012. Epub 2009 Nov 26. [Article]