Serpin B6

Details

Name

Serpin B6

Synonyms

• CAP
• Cytoplasmic antiproteinase
• Peptidase inhibitor 6
• PI-6
• PI6
• Placental thrombin inhibitor
• PTI

Gene Name

SERPINB6

Organism

Humans

Amino acid sequence

>lcl|BSEQ0037166|Serpin B6
MDVLAEANGTFALNLLKTLGKDNSKNVFFSPMSMSCALAMVYMGAKGNTAAQMAQILSFN
KSGGGGDIHQGFQSLLTEVNKTGTQYLLRMANRLFGEKSCDFLSSFRDSCQKFYQAEMEE
LDFISAVEKSRKHINTWVAEKTEGKIAELLSPGSVDPLTRLVLVNAVYFRGNWDEQFDKE
NTEERLFKVSKNEEKPVQMMFKQSTFKKTYIGEIFTQILVLPYVGKELNMIIMLPDETTD
LRTVEKELTYEKFVEWTRLDMMDEEEVEVSLPRFKLEESYDMESVLRNLGMTDAFELGKA
DFSGMSQTDLSLSKVVHKSFVEVNEEGTEAAAATAAIMMMRCARFVPRFCADHPFLFFIQ
HSKTNGILFCGRFSSP

Number of residues

376

Molecular Weight

42621.575

Theoretical pI

4.93

GO Classification

Functions

protease binding / serine-type endopeptidase inhibitor activity

Processes

cellular response to osmotic stress / negative regulation of endopeptidase activity / sensory perception of sound

Components

cytoplasm / cytosol / extracellular exosome / extracellular space / protein complex

General Function

Serine-type endopeptidase inhibitor activity

Specific Function

May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood (By similarity). Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress and loss of this protection results in cell death and sensorineural hearing loss.

Pfam Domain Function

• Serpin (PF00079)

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0011553|Serpin B6 (SERPINB6)
ATGTCTGCCATCATGGATGTTCTCGCAGAAGCAAATGGCACCTTTGCCTTAAACCTTTTG
AAAACGCTGGGTAAAGACAACTCGAAGAATGTGTTTTTCTCACCCATGAGCATGTCCTGT
GCCCTGGCCATGGTCTACATGGGGGCAAAGGGAAACACCGCTGCACAGATGGCCCAGATA
CTTTCTTTCAATAAAAGTGGCGGTGGTGGAGACATCCACCAGGGCTTCCAGTCTCTTCTC
ACCGAAGTGAACAAGACTGGCACGCAGTACTTGCTTAGGATGGCCAACAGGCTCTTTGGG
GAAAAGTCTTGTGATTTCCTCTCATCTTTTAGAGATTCCTGCCAAAAATTCTACCAAGCA
GAGATGGAGGAGCTTGACTTTATCAGCGCCGTAGAGAAGTCCAGAAAACACATAAACACC
TGGGTAGCTGAAAAGACAGAAGGTAAAATTGCGGAGTTGCTCTCTCCGGGCTCAGTGGAT
CCATTGACAAGGCTGGTTCTGGTGAATGCTGTCTATTTCAGAGGAAACTGGGATGAACAG
TTTGACAAGGAGAACACCGAGGAGAGACTGTTTAAAGTCAGCAAGAATGAGGAGAAACCT
GTGCAAATGATGTTTAAGCAATCTACTTTTAAGAAGACCTATATAGGAGAAATATTTACC
CAAATCTTGGTGCTTCCATATGTTGGCAAGGAACTGAATATGATCATCATGCTTCCGGAC
GAGACCACTGACTTGAGAACGGTGGAGAAAGAACTCACTTACGAGAAGTTCGTAGAATGG
ACGAGGCTGGACATGATGGATGAAGAGGAGGTGGAAGTGTCCCTCCCGCGGTTTAAACTA
GAGGAAAGCTACGACATGGAGAGTGTCCTGCGCAACCTGGGCATGACTGATGCCTTCGAG
CTGGGCAAGGCAGACTTCTCTGGAATGTCCCAGACAGACCTGTCTCTGTCCAAGGTCGTG
CACAAGTCTTTTGTGGAGGTCAATGAGGAAGGCACGGAGGCTGCAGCCGCCACAGCTGCC
ATCATGATGATGCGGTGTGCCAGATTCGTCCCCCGCTTCTGCGCCGACCACCCCTTCCTT
TTCTTCATCCAGCACAGCAAGACCAACGGGATTCTCTTCTGCGGCCGCTTTTCCTCTCCG
TGA

Chromosome Location

6

Locus

6p25

External Identifiers

Resource	Link
UniProtKB ID	P35237
UniProtKB Entry Name	SPB6_HUMAN
GenBank Protein ID	297412
GenBank Gene ID	Z22658
GenAtlas ID	SERPINB6
HGNC ID	HGNC:8950

General References

1. Coughlin P, Sun J, Cerruti L, Salem HH, Bird P: Cloning and molecular characterization of a human intracellular serine proteinase inhibitor. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9417-21. [Article]
2. Morgenstern KA, Sprecher C, Holth L, Foster D, Grant FJ, Ching A, Kisiel W: Complementary DNA cloning and kinetic characterization of a novel intracellular serine proteinase inhibitor: mechanism of action with trypsin and factor Xa as model proteinases. Biochemistry. 1994 Mar 22;33(11):3432-41. [Article]
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6. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
7. Coughlin PB, Tetaz T, Salem HH: Identification and purification of a novel serine proteinase inhibitor. J Biol Chem. 1993 May 5;268(13):9541-7. [Article]
8. Scott FL, Hirst CE, Sun J, Bird CH, Bottomley SP, Bird PI: The intracellular serpin proteinase inhibitor 6 is expressed in monocytes and granulocytes and is a potent inhibitor of the azurophilic granule protease, cathepsin G. Blood. 1999 Mar 15;93(6):2089-97. [Article]
9. Strik MC, Wolbink A, Wouters D, Bladergroen BA, Verlaan AR, van Houdt IS, Hijlkema S, Hack CE, Kummer JA: Intracellular serpin SERPINB6 (PI6) is abundantly expressed by human mast cells and forms complexes with beta-tryptase monomers. Blood. 2004 Apr 1;103(7):2710-7. Epub 2003 Dec 11. [Article]
10. Scott FL, Sun J, Whisstock JC, Kato K, Bird PI: SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes. J Biochem. 2007 Oct;142(4):435-42. Epub 2007 Aug 30. [Article]
11. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [Article]
12. Sirmaci A, Erbek S, Price J, Huang M, Duman D, Cengiz FB, Bademci G, Tokgoz-Yilmaz S, Hismi B, Ozdag H, Ozturk B, Kulaksizoglu S, Yildirim E, Kokotas H, Grigoriadou M, Petersen MB, Shahin H, Kanaan M, King MC, Chen ZY, Blanton SH, Liu XZ, Zuchner S, Akar N, Tekin M: A truncating mutation in SERPINB6 is associated with autosomal-recessive nonsyndromic sensorineural hearing loss. Am J Hum Genet. 2010 May 14;86(5):797-804. doi: 10.1016/j.ajhg.2010.04.004. Epub 2010 May 6. [Article]
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15. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB00055	Drotrecogin alfa	approved, investigational, withdrawn	unknown		Details