Beta-phosphoglucomutase
Details
- Name
- Beta-phosphoglucomutase
- Synonyms
- 5.4.2.6
- Beta-PGM
- Gene Name
- pgmB
- Organism
- Lactococcus lactis subsp. lactis (strain IL1403)
- Amino acid sequence
>lcl|BSEQ0010948|Beta-phosphoglucomutase MFKAVLFDLDGVITDTAEYHFRAWKALAEEIGINGVDRQFNEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNYVKMIQDVSPADVYPGILQLLKDLRSNKIKIALASASKNGP FLLEKMNLTGYFDAIADPAEVAASKPAPDIFIAAAHAVGVAPSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVIVPDTSYYTLEFLKEVWLQKQK
- Number of residues
- 221
- Molecular Weight
- 24208.37
- Theoretical pI
- 4.6
- GO Classification
- Functionsbeta-phosphoglucomutase activity / hydrolase activity / magnesium ion bindingProcessescarbohydrate metabolic processComponentscytoplasm
- General Function
- Magnesium ion binding
- Specific Function
- Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.
- Pfam Domain Function
- HAD_2 (PF13419)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0010949|Beta-phosphoglucomutase (pgmB) ATGTTTAAAGCAGTATTGTTTGATTTAGATGGTGTAATTACAGATACCGCAGAGTATCAT TTTAGAGCTTGGAAAGCTTTGGCTGAAGAAATTGGCATTAATGGTGTTGACCGCCAATTT AATGAGCAATTAAAAGGGGTCTCACGAGAAGACTCGCTTCAGAAAATTCTAGATTTAGCT GATAAAAAAGTATCAGCTGAGGAATTTAAAGAACTTGCTAAGAGAAAAAATGATAACTAT GTGAAAATGATTCAGGATGTGTCGCCAGCCGATGTCTATCCTGGAATTTTACAATTACTC AAAGATTTACGTTCAAATAAAATCAAAATTGCTTTAGCATCGGCTTCTAAGAATGGTCCA TTTTTATTAGAGAAAATGAATTTAACTGGATATTTTGATGCAATTGCTGATCCGGCTGAA GTTGCAGCATCAAAACCAGCACCAGATATTTTTATTGCAGCAGCACATGCAGTGGGTGTT GCCCCCTCTGAATCAATTGGGTTAGAGGATTCTCAAGCTGGAATTCAAGCCATCAAAGAT TCAGGGGCTTTACCAATTGGTGTAGGGCGCCCAGAAGATTTGGGAGATGATATCGTCATT GTGCCTGATACTTCATACTATACATTAGAATTTTTGAAAGAAGTTTGGCTTCAAAAGCAA AAATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P71447 UniProtKB Entry Name PGMB_LACLA GenBank Protein ID 1495997 GenBank Gene ID Z70730 - General References
- Qian N, Stanley GA, Bunte A, Radstrom P: Product formation and phosphoglucomutase activities in Lactococcus lactis: cloning and characterization of a novel phosphoglucomutase gene. Microbiology. 1997 Mar;143 ( Pt 3):855-65. [Article]
- Bolotin A, Wincker P, Mauger S, Jaillon O, Malarme K, Weissenbach J, Ehrlich SD, Sorokin A: The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome Res. 2001 May;11(5):731-53. [Article]
- Qian N, Stanley GA, Hahn-Hagerdal B, Radstrom P: Purification and characterization of two phosphoglucomutases from Lactococcus lactis subsp. lactis and their regulation in maltose- and glucose-utilizing cells. J Bacteriol. 1994 Sep;176(17):5304-11. [Article]
- Lahiri SD, Zhang G, Dai J, Dunaway-Mariano D, Allen KN: Analysis of the substrate specificity loop of the HAD superfamily cap domain. Biochemistry. 2004 Mar 16;43(10):2812-20. [Article]
- Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN: Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis. Biochemistry. 2002 Jul 2;41(26):8351-9. [Article]
- Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN: The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction. Science. 2003 Mar 28;299(5615):2067-71. Epub 2003 Mar 13. [Article]
- Zhang G, Dai J, Wang L, Dunaway-Mariano D, Tremblay LW, Allen KN: Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis. Biochemistry. 2005 Jul 12;44(27):9404-16. [Article]
- Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN: Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. J Am Chem Soc. 2005 Apr 20;127(15):5298-9. [Article]
- Dai J, Finci L, Zhang C, Lahiri S, Zhang G, Peisach E, Allen KN, Dunaway-Mariano D: Analysis of the structural determinants underlying discrimination between substrate and solvent in beta-phosphoglucomutase catalysis. Biochemistry. 2009 Mar 10;48(9):1984-95. doi: 10.1021/bi801653r. [Article]
- Baxter NJ, Bowler MW, Alizadeh T, Cliff MJ, Hounslow AM, Wu B, Berkowitz DB, Williams NH, Blackburn GM, Waltho JP: Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by MgF-3 rather than by phosphoranes. Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4555-60. doi: 10.1073/pnas.0910333106. Epub 2010 Feb 17. [Article]