4-hydroxy-tetrahydrodipicolinate reductase
Details
- Name
- 4-hydroxy-tetrahydrodipicolinate reductase
- Synonyms
- 1.17.1.8
- HTPA reductase
- Gene Name
- dapB
- Organism
- Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- Amino acid sequence
>lcl|BSEQ0051134|4-hydroxy-tetrahydrodipicolinate reductase MRVGVLGAKGKVGATMVRAVAAADDLTLSAELDAGDPLSLLTDGNTEVVIDFTHPDVVMG NLEFLIDNGIHAVVGTTGFTAERFQQVESWLVAKPNTSVLIAPNFAIGAVLSMHFAKQAA RFFDSAEVIELHHPHKADAPSGTAARTAKLIAEARKGLPPNPDATSTSLPGARGADVDGI PVHAVRLAGLVAHQEVLFGTEGETLTIRHDSLDRTSFVPGVLLAVRRIAERPGLTVGLEP LLDLH
- Number of residues
- 245
- Molecular Weight
- 25733.14
- Theoretical pI
- Not Available
- GO Classification
- Functions4-hydroxy-tetrahydrodipicolinate reductase / NADH binding / NADPH bindingProcessesdiaminopimelate biosynthetic process / lysine biosynthetic process via diaminopimelateComponentscell wall / cytosol / plasma membrane
- General Function
- Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate (Probable). Can use both NADH and NADPH as a reductant, with NADH being 6-fold as effective as NADPH.
- Specific Function
- 4-hydroxy-tetrahydrodipicolinate reductase
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0051135|4-hydroxy-tetrahydrodipicolinate reductase (dapB) ATGCGGGTAGGCGTCCTTGGAGCCAAAGGCAAGGTCGGAGCGACGATGGTGCGGGCGGTG GCGGCCGCCGACGACCTGACCCTATCCGCCGAGCTGGATGCCGGCGATCCGCTGAGCCTG CTAACGGACGGTAACACCGAGGTCGTCATCGACTTCACCCACCCGGACGTGGTGATGGGC AATCTGGAGTTCCTCATCGACAACGGAATTCACGCCGTGGTCGGTACCACGGGGTTCACC GCCGAGCGGTTTCAACAAGTCGAATCGTGGCTCGTCGCAAAACCCAACACATCGGTGTTG ATAGCGCCAAACTTCGCGATCGGAGCGGTGCTGTCCATGCATTTCGCCAAGCAGGCCGCA CGGTTTTTCGACTCGGCCGAGGTCATTGAGCTGCATCATCCGCACAAGGCTGACGCGCCG TCAGGCACGGCCGCGCGTACCGCGAAGCTGATCGCCGAGGCCCGAAAAGGCTTGCCGCCC AATCCCGATGCCACCAGTACCAGCCTGCCGGGCGCGCGTGGTGCCGACGTCGACGGCATA CCGGTGCACGCGGTGCGGCTGGCCGGACTGGTCGCCCACCAGGAGGTGCTGTTCGGGACC GAGGGGGAGACTCTGACCATCCGCCACGATAGCCTCGATCGCACATCGTTTGTGCCCGGT GTGCTGTTGGCGGTGCGCCGCATCGCCGAACGCCCTGGTCTCACCGTAGGTCTTGAGCCC CTACTCGATCTGCACTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P9WP23 UniProtKB Entry Name DAPB_MYCTU - General References
- Pavelka MS Jr, Weisbrod TR, Jacobs WR Jr: Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis. J Bacteriol. 1997 Apr;179(8):2777-82. [Article]
- Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [Article]
- Kefala G, Janowski R, Panjikar S, Mueller-Dieckmann C, Weiss MS: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt 7):718-21. Epub 2005 Jun 30. [Article]
- Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [Article]
- Cirilli M, Zheng R, Scapin G, Blanchard JS: The three-dimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and -NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity. Biochemistry. 2003 Sep 16;42(36):10644-50. [Article]
- Janowski R, Kefala G, Weiss MS: The structure of dihydrodipicolinate reductase (DapB) from Mycobacterium tuberculosis in three crystal forms. Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):61-72. doi: 10.1107/S0907444909043960. Epub 2009 Dec 21. [Article]