Carboxylesterase
Details
- Name
- Carboxylesterase
- Synonyms
- 3.1.1.1
- est30
- Gene Name
- est
- Organism
- Geobacillus stearothermophilus
- Amino acid sequence
>lcl|BSEQ0037140|Carboxylesterase MKIVPPKPFFFEAGERAVLLLHGFTGNSADVRMLGRFLESKGYTCHAPIYKGHGVPPEEL VHTGPDDWWQDVMNGYEFLKNKGYEKIAVAGLSLGGVFSLKLGYTVPIEGIVTMCAPMYI KSEETMYEGVLEYAREYKKREGKSEEQIEQEMEKFKQTPMKTLKALQELIADVRDHLDLI YAPTFVVQARHDEMINPDSANIIYNEIESPVKQIKWYEQSGHVITLDQEKDQLHEDIYAF LESLDW
- Number of residues
- 246
- Molecular Weight
- 28256.125
- Theoretical pI
- 4.79
- GO Classification
- Functionscarboxylic ester hydrolase activity
- General Function
- Carboxylic ester hydrolase activity
- Specific Function
- Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones.
- Pfam Domain Function
- Hydrolase_4 (PF12146)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0002518|744 bp ATGATGAAAATTGTTCCGCCGAAGCCGTTTTTCTTTGAAGCCGGGGAGCGGGCGGTGCTG CTGTTGCATGGGTTTACCGGCAATTCCGCTGATGTTCGGATGCTCGGGCGTTTTTTAGAA TCCAAAGGCTATACGTGCCATGCGCCTATTTACAAAGGACACGGCGTGCCGCCTGAGGAG CTCGTCCACACCGGGCCGGATGACTGGTGGCAAGATGTCATGAACGGCTACGAGTTTTTG AAAAACAAGGGCTACGAAAAAATCGCCGTCGCCGGACTGTCGCTTGGAGGCGTATTTTCA TTGAAATTAGGTTACACTGTACCTATAGAGGGCATTGTGACGATGTGCGCGCCGATGTAC ATCAAAAGCGAGGAAACGATGTACGAAGGCGTGCTCGAGTATGCGCGCGAGTATAAAAAG CGGGAAGGAAAATCAGAGGAGCAGATCGAACAGGAGATGGAGAAGTTCAAGCAGACGCCG ATGAAGACGTTAAAGGCGCTGCAGGAGCTGATCGCCGATGTGCGTGACCATCTTGATTTG ATTTATGCCCCGACGTTTGTTGTGCAGGCGCGCCATGATGAGATGATCAACCCGGACAGC GCGAACATCATTTATAACGAAATTGAATCGCCGGTCAAACAAATCAAGTGGTATGAGCAA TCAGGCCATGTGATTACGCTTGATCAAGAAAAAGATCAGCTGCATGAAGATATTTATGCA TTTCTTGAATCGTTAGATTGGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q06174 UniProtKB Entry Name EST_GEOSE GenBank Protein ID 216314 GenBank Gene ID D12681 - General References
- Kugimiya W, Otani Y, Hashimoto Y: Molecular cloning and structure of the gene for esterase from a thermophilic bacterium, Bacillus stearothermophilus IFO 12550. Biosci Biotechnol Biochem. 1992 Dec;56(12):2074-5. [Article]
- Ewis HE, Abdelal AT, Lu CD: Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus. Gene. 2004 Mar 31;329:187-95. [Article]
- Liu P, Wang YF, Ewis HE, Abdelal AT, Lu CD, Harrison RW, Weber IT: Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30. J Mol Biol. 2004 Sep 10;342(2):551-61. [Article]