Splicing factor 3B subunit 3

Details

Name

Splicing factor 3B subunit 3

Synonyms

• KIAA0017
• Pre-mRNA-splicing factor SF3b 130 kDa subunit
• SAP 130
• SAP130
• SF3b130
• Spliceosome-associated protein 130
• STAF130

Gene Name

SF3B3

Organism

Humans

Amino acid sequence

>lcl|BSEQ0049753|Splicing factor 3B subunit 3
MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFG
VIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQF
LAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENP
MFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPG
GSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFF
FLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQ
IAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDT
PQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVN
ATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPG
KKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQ
RSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSI
GFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSW
LSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFP
LQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAA
AFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTG
EDWYVLVGVAKDLILNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVL
IGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQ
LIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRG
LLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDF
FQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRT
PPEVSKKLEDIRTRYAF

Number of residues

1217

Molecular Weight

135576.25

Theoretical pI

Not Available

GO Classification

Functions

protein complex binding / RNA binding

Processes

mRNA processing / mRNA splicing, via spliceosome / negative regulation of protein catabolic process / protein complex assembly / RNA splicing / RNA splicing, via transesterification reactions

Components

catalytic step 2 spliceosome / nucleolus / nucleoplasm / nucleus / small nuclear ribonucleoprotein complex / spliceosomal complex / U12-type spliceosomal complex

General Function

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Specific Function

Protein complex binding

Pfam Domain Function

• CPSF_A (PF03178)

Transmembrane Regions

Not Available

Cellular Location

Nucleus

Gene sequence

>lcl|BSEQ0049754|Splicing factor 3B subunit 3 (SF3B3)
ATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTCATGGA
AACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGGAGCTG
CTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTATTCGGT
GTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTGTAGTT
GGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGTTTGAG
AAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCCAGTTC
TTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGAAATTG
GTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAAGCC
CACAAAGCAAACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAATCCA
ATGTTTGCTTGTCTGGAAATGGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCA
GCAGCTAATACCCAGCAGACACTTACTTTCTATGAGCTAGACCTTGGTTTAAATCATGTG
GTCCGAAAATACAGTGAACCTTTGGAGGAACACGGCAACTTCCTTATTACAGTTCCAGGA
GGGTCAGATGGTCCAAGTGGAGTACTGATCTGCTCTGAAAACTATATTACTTACAAGAAC
TTTGGTGACCAGCCAGATATCCGCTGTCCAATTCCCAGGAGGCGGAATGACCTGGATGAC
CCTGAAAGAGGAATGATTTTTGTCTGCTCTGCAACCCATAAAACCAAATCGATGTTCTTC
TTTTTGGCTCAAACTGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGAT
ATGGTTACTGAGATCCGGCTCAAATATTTTGATACTGTACCCGTTGCTGCTGCCATGTGT
GTGCTTAAAACAGGGTTCCTTTTTGTAGCATCAGAATTTGGAAACCATTACTTATATCAA
ATTGCACATCTTGGAGATGATGATGAAGAACCTGAGTTTTCATCAGCCATGCCTCTGGAA
GAAGGAGACACATTCTTTTTTCAGCCAAGACCACTTAAAAACCTTGTGCTGGTTGATGAG
TTGGACAGCCTCTCTCCCATTCTGTTTTGCCAGATAGCTGATCTGGCCAATGAAGATACT
CCACAGTTGTATGTGGCCTGTGGTAGGGGACCCCGATCATCTCTGAGAGTCCTAAGACAT
GGACTTGAGGTGTCAGAAATGGCTGTTTCTGAGCTACCTGGTAACCCCAACGCTGTCTGG
ACAGTGCGTCGACACATTGAAGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAAT
GCCACCCTAGTGTTGTCCATTGGAGAAACTGTAGAAGAAGTGACTGACTCTGGGTTCCTG
GGGACCACCCCGACCTTGTCCTGCTCCTTATTAGGAGATGATGCCTTGGTGCAGGTCTAT
CCAGATGGCATTCGGCACATACGAGCAGACAAGAGAGTCAATGAGTGGAAGACCCCTGGA
AAGAAAACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATTGCCCTGACAGGA
GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGCTGAATGAGTACACAGAACGG
AAGGAGATGTCAGCAGATGTGGTGTGCATGAGTCTGGCCAATGTACCCCCTGGAGAGCAG
CGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGACAACACTGTCAGAATCATCTCCCTGGAT
CCCTCAGACTGTTTGCAACCTCTAAGCATGCAGGCTCTCCCAGCCCAGCCTGAGTCCTTG
TGTATCGTGGAAATGGGTGGGACTGAGAAGCAGGATGAGCTGGGTGAGAGGGGCTCGATT
GGCTTCCTATACCTGAATATTGGGCTACAGAACGGTGTGCTGCTGAGGACTGTCTTGGAC
CCTGTCACTGGGGATTTGTCTGATACTCGCACTCGGTACCTGGGGTCCCGTCCTGTGAAG
CTCTTCCGAGTCCGAATGCAAGGCCAGGAGGCAGTATTGGCCATGTCAAGCCGCTCATGG
TTGAGCTATTCTTACCAATCTCGCTTCCATCTCACCCCACTGTCTTACGAGACACTGGAA
TTTGCATCGGGTTTTGCCTCGGAACAGTGTCCCGAGGGCATTGTGGCCATCTCCACCAAC
ACCCTACGGATTTTGGCATTAGAGAAGCTCGGTGCTGTCTTCAATCAAGTAGCCTTCCCA
CTGCAGTACACACCCAGGAAATTTGTCATCCACCCTGAGAGTAACAACCTTATTATCATT
GAAACGGACCACAATGCCTACACTGAGGCCACGAAAGCTCAGAGAAAGCAGCAGATGGCA
GAGGAAATGGTGGAAGCAGCAGGGGAGGATGAGCGGGAGCTGGCCGCAGAGATGGCAGCA
GCATTCCTCAATGAAAACCTCCCTGAATCCATCTTTGGAGCTCCCAAGGCTGGCAATGGG
CAGTGGGCCTCTGTGATCCGAGTGATGAATCCCATTCAAGGGAACACACTGGACCTTGTC
CAGCTGGAACAGAATGAGGCAGCTTTTAGTGTGGCTGTGTGCAGGTTTTCCAACACTGGT
GAAGACTGGTATGTGCTGGTGGGTGTGGCCAAGGACCTGATACTAAACCCCCGATCTGTG
GCAGGGGGCTTCGTCTATACTTACAAGCTTGTGAACAATGGGGAAAAACTGGAGTTTTTG
CACAAGACTCCTGTGGAAGAGGTCCCTGCTGCTATTGCCCCATTCCAGGGGAGGGTGTTG
ATTGGTGTGGGGAAGCTGTTGCGTGTCTATGACCTGGGAAAGAAGAAGTTACTCCGAAAA
TGTGAGAATAAGCATATTGCCAATTATATCTCTGGGATCCAGACTATCGGACATAGGGTA
ATTGTATCTGATGTCCAAGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAAAACCAG
CTTATCATCTTTGCTGATGATACCTACCCCCGATGGGTCACTACAGCCAGCCTCCTGGAC
TATGACACTGTGGCTGGGGCAGACAAGTTTGGCAACATATGTGTGGTGAGGCTCCCACCT
AACACCAATGATGAAGTAGATGAGGATCCTACAGGAAACAAAGCCCTGTGGGACCGTGGC
TTGCTCAATGGGGCCTCCCAGAAGGCAGAGGTGATCATGAACTACCATGTCGGGGAGACG
GTGCTGTCCTTGCAGAAGACCACGCTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC
ACCTTGTCTGGAGGAATTGGCATCCTTGTGCCATTCACGTCCCATGAGGACCATGACTTC
TTCCAGCATGTGGAAATGCACCTGCGGTCTGAACATCCCCCTCTCTGTGGGCGGGACCAC
CTCAGCTTTCGCTCCTACTACTTCCCTGTGAAGAATGTGATTGATGGAGACCTCTGTGAG
CAGTTCAATTCCATGGAACCCAACAAACAAAAGAACGTCTCTGAAGAACTGGACCGAACC
CCACCCGAAGTGTCCAAGAAACTCGAGGATATCCGGACCCGCTACGCCTTCTGA

Chromosome Location

16

Locus

16q22.1

External Identifiers

Resource	Link
UniProtKB ID	Q15393
UniProtKB Entry Name	SF3B3_HUMAN
HGNC ID	HGNC:10770

General References

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2. Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S: Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1. DNA Res. 1994;1(1):27-35. [Article]
3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
4. Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007 Oct 31;8:399. [Article]
5. Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG: Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. Mol Cell Biol. 2001 Oct;21(20):6782-95. [Article]
6. Gangloff YG, Pointud JC, Thuault S, Carre L, Romier C, Muratoglu S, Brand M, Tora L, Couderc JL, Davidson I: The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger. Mol Cell Biol. 2001 Aug;21(15):5109-21. [Article]
7. Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Luhrmann R: Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. EMBO J. 2002 Sep 16;21(18):4978-88. [Article]
8. Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ: Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA. 2002 Apr;8(4):426-39. [Article]
9. Golas MM, Sander B, Will CL, Luhrmann R, Stark H: Molecular architecture of the multiprotein splicing factor SF3b. Science. 2003 May 9;300(5621):980-4. [Article]
10. Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Luhrmann R: The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA. 2004 Jun;10(6):929-41. [Article]
11. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
12. Bienvenut WV, Sumpton D, Martinez A, Lilla S, Espagne C, Meinnel T, Giglione C: Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-alpha-acetylation features. Mol Cell Proteomics. 2012 Jun;11(6):M111.015131. doi: 10.1074/mcp.M111.015131. Epub 2012 Jan 5. [Article]
13. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54. doi: 10.1073/pnas.1210303109. Epub 2012 Jul 18. [Article]
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15. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB04216	Quercetin	experimental, investigational	unknown		Details