Splicing factor 1

Details

Name

Splicing factor 1

Synonyms

• BBP
• Mammalian branch point-binding protein
• mBBP
• Transcription factor ZFM1
• ZFM1
• Zinc finger gene in MEN1 locus
• Zinc finger protein 162
• ZNF162

Gene Name

SF1

Organism

Humans

Amino acid sequence

>lcl|BSEQ0049944|Splicing factor 1
MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIED
LTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALN
PDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGK
GSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLR
KMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDP
QSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMS
TTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGP
PPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPP
PPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGS
IPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPP
PPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN

Number of residues

639

Molecular Weight

68329.55

Theoretical pI

Not Available

GO Classification

Functions

identical protein binding / pre-mRNA branch point binding / RNA binding / transcription corepressor activity / zinc ion binding

Processes

Leydig cell differentiation / male sex determination / mRNA 3'-splice site recognition / mRNA splicing, via spliceosome / negative regulation of smooth muscle cell proliferation / nuclear body organization / regulation of steroid biosynthetic process / regulation of transcription, DNA-templated / spliceosomal complex assembly / transcription, DNA-templated

Components

nuclear body / nucleoplasm / nucleus / ribosome / spliceosomal complex

General Function

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Specific Function

Identical protein binding

Pfam Domain Function

• zf-CCHC (PF00098)
• KH_1 (PF00013)
• SF1-HH (PF16275)

Transmembrane Regions

Not Available

Cellular Location

Nucleus

Gene sequence

>lcl|BSEQ0049945|Splicing factor 1 (SF1)
ATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCCAAGTAAGAAGCGGAAGAGGAGC
CGCTGGAACCAAGACACAATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATT
CCCCCTGGACTTACTCGAGAACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGAC
CTGACTCGTAAACTGCGCACAGGAGACCTGGGCATCCCCCCTAACCCTGAGGACAGGTCC
CCTTCCCCTGAGCCCATCTACAATAGCGAGGGGAAGCGGCTTAACACCCGAGAGTTCCGC
ACCCGCAAAAAGCTGGAAGAGGAGCGGCACAACCTCATCACAGAGATGGTTGCACTCAAT
CCGGATTTCAAGCCACCTGCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTC
ATGATTCCACAAGATGAGTACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGA
GGGAACACCCTGAAGAACATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAA
GGGTCTGTGAAAGAAGGGAAGGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGAT
GAGCCACTTCATGCCCTGGTTACTGCCAATACAATGGAGAACGTCAAAAAGGCAGTGGAA
CAGATAAGAAACATCCTGAAGCAGGGTATCGAGACTCCAGAGGACCAGAATGATCTACGG
AAGATGCAGCTTCGGGAGTTGGCTCGCTTAAATGGGACCCTTCGGGAAGACGATAACAGG
ATCTTAAGACCCTGGCAGAGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACC
AAGTGTGGAGGGGCTGGCCACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCT
CAGTCAGCTCAGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTG
GGTGAAGCACCTGTCCCAGCATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTG
GCCAGCGCACCTCGTCCTGCTGCTCCCGCCAACAACCCACCTCCACCGTCTCTCATGTCT
ACCACCCAGAGCCGCCCACCCTGGATGAATTCTGGCCCTTCAGAGAGTCGGCCCTACCAC
GGCATGCATGGAGGTGGTCCTGGTGGGCCCGGAGGTGGCCCCCACAGCTTCCCACACCCA
TTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCC
CCACCCCCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGG
CACCATGGCCCTCCTCCAATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTC
TATCGCCTGCATCAAGGAAAAGGTATGATGCCGCCACCACCTATGGGCATGATGCCGCCG
CCGCCGCCGCCTCCCAGTGGGCAGCCCCCACCCCCTCCCTCTGGTCCTCTTCCCCCATGG
CAACAACAGCAGCAGCAGCCTCCGCCACCCCCTCCGCCCAGCAGCAGTATGGCTTCCAGT
ACCCCCTTGCCATGGCAGCAAAATACGACGACTACCACCACGAGCGCTGGCACAGGGTCC
ATCCCGCCATGGCAACAGCAGCAGGCGGCTGCCGCAGCTTCTCCAGGAGCCCCTCAGATG
CAAGGCAACCCCACTATGGTGCCCCTGCCCCCCGGGGTCCAGCCGCCTCTGCCGCCTGGG
GCCCCTCCCCCTCCGCCGCCTCCACCGCCTGGTTCCGCCGGCATGATGTATGCCCCGCCC
CCTCCTCCTCCGCCTCCCATGGACCCTTCTAACTTTGTCACCATGATGGGCATGGGGGTG
GCGGGCATGCCGCCCTTCGGGATGCCTCCAGCTCCCCCACCGCCTCCACCACAGAACTAG

Chromosome Location

11

Locus

11q13.1

External Identifiers

Resource	Link
UniProtKB ID	Q15637
UniProtKB Entry Name	SF01_HUMAN
HGNC ID	HGNC:12950

General References

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8. Wang X, Bruderer S, Rafi Z, Xue J, Milburn PJ, Kramer A, Robinson PJ: Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly. EMBO J. 1999 Aug 16;18(16):4549-59. [Article]
9. Rappsilber J, Ryder U, Lamond AI, Mann M: Large-scale proteomic analysis of the human spliceosome. Genome Res. 2002 Aug;12(8):1231-45. [Article]
10. Zhang D, Paley AJ, Childs G: The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription. J Biol Chem. 1998 Jul 17;273(29):18086-91. [Article]
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12. Beausoleil SA, Villen J, Gerber SA, Rush J, Gygi SP: A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol. 2006 Oct;24(10):1285-92. Epub 2006 Sep 10. [Article]
13. Corsini L, Bonnal S, Basquin J, Hothorn M, Scheffzek K, Valcarcel J, Sattler M: U2AF-homology motif interactions are required for alternative splicing regulation by SPF45. Nat Struct Mol Biol. 2007 Jul;14(7):620-9. Epub 2007 Jun 24. [Article]
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Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB11638	Artenimol	approved, experimental, investigational	unknown	ligand	Details