Phenylacetone monooxygenase
Details
- Name
- Phenylacetone monooxygenase
- Synonyms
- 1.14.13.92
- Baeyer-Villiger monooxygenase
- BVMO
- PAMO
- Gene Name
- pamO
- Organism
- Thermobifida fusca (strain YX)
- Amino acid sequence
>lcl|BSEQ0012092|Phenylacetone monooxygenase MAGQTTVDSRRQPPEEVDVLVVGAGFSGLYALYRLRELGRSVHVIETAGDVGGVWYWNRY PGARCDIESIEYCYSFSEEVLQEWNWTERYASQPEILRYINFVADKFDLRSGITFHTTVT AAAFDEATNTWTVDTNHGDRIRARYLIMASGQLSVPQLPNFPGLKDFAGNLYHTGNWPHE PVDFSGQRVGVIGTGSSGIQVSPQIAKQAAELFVFQRTPHFAVPARNAPLDPEFLADLKK RYAEFREESRNTPGGTHRYQGPKSALEVSDEELVETLERYWQEGGPDILAAYRDILRDRD ANERVAEFIRNKIRNTVRDPEVAERLVPKGYPFGTKRLILEIDYYEMFNRDNVHLVDTLS APIETITPRGVRTSEREYELDSLVLATGFDALTGALFKIDIRGVGNVALKEKWAAGPRTY LGLSTAGFPNLFFIAGPGSPSALSNMLVSIEQHVEWVTDHIAYMFKNGLTRSEAVLEKED EWVEHVNEIADETLYPMTASWYTGANVPGKPRVFMLYVGGFHRYRQICDEVAAKGYEGFV LT
- Number of residues
- 542
- Molecular Weight
- 61123.225
- Theoretical pI
- 5.0
- GO Classification
- Functionsphenylacetone monooxygenase activity
- General Function
- Phenylacetone monooxygenase activity
- Specific Function
- Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Is most efficient with phenylacetone as substrate, leading to the formation of benzyl acetate. Can also oxidize other aromatic ketones (benzylacetone, alpha-methylphenylacetone and 4-hydroxyacetophenone), some aliphatic ketones (dodecan-2-one and bicyclohept-2-en-6-one) and sulfides (e.g. methyl 4-tolylsulfide).
- Pfam Domain Function
- DAO (PF01266)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0012093|Phenylacetone monooxygenase (pamO) ATGGCCGGGCAGACGACTGTCGACTCTCGCCGACAACCACCAGAGGAAGTGGACGTCCTG GTCGTGGGAGCCGGCTTCTCCGGCCTGTACGCCCTCTACCGTCTGCGGGAACTCGGGCGT AGCGTGCACGTCATCGAAACCGCTGGCGACGTGGGCGGTGTGTGGTACTGGAACCGCTAC CCGGGGGCGCGGTGCGACATCGAGAGCATCGAGTACTGCTACTCGTTCTCCGAGGAGGTC CTCCAAGAGTGGAACTGGACTGAGCGGTACGCTTCCCAGCCCGAGATCCTGCGCTACATC AACTTCGTCGCCGACAAATTCGACCTGCGGAGCGGAATCACCTTCCACACCACGGTGACC GCGGCAGCCTTCGACGAGGCCACCAACACGTGGACGGTCGACACCAACCACGGCGACCGG ATCCGCGCCCGCTACCTCATCATGGCCAGCGGCCAGCTCTCCGTCCCGCAGCTCCCCAAC TTCCCAGGGCTCAAAGACTTCGCCGGCAACCTCTACCACACCGGGAACTGGCCGCACGAG CCCGTGGACTTCTCCGGGCAGCGGGTGGGCGTGATCGGCACCGGGTCGTCCGGAATCCAG GTGAGCCCACAGATCGCGAAACAGGCTGCGGAACTGTTCGTGTTCCAGCGCACCCCCCAC TTTGCCGTCCCCGCCCGCAACGCGCCGCTCGACCCGGAGTTCCTCGCCGACCTGAAGAAG CGCTACGCCGAATTCCGGGAAGAGTCCCGCAACACTCCCGGAGGAACCCACCGCTACCAG GGGCCGAAGTCCGCGCTGGAAGTCAGCGACGAAGAGCTGGTGGAAACCCTGGAACGCTAC TGGCAGGAGGGCGGCCCCGACATTCTGGCCGCCTACCGCGACATCCTGCGCGACCGGGAC GCCAACGAACGGGTCGCTGAATTCATCCGCAACAAGATCCGCAACACCGTGCGCGACCCG GAGGTGGCGGAACGCCTGGTCCCCAAGGGCTACCCGTTCGGCACCAAGCGCCTCATCCTG GAAATCGACTACTACGAAATGTTCAACCGGGACAACGTGCACCTGGTCGACACGTTGTCT GCGCCGATCGAGACGATCACGCCGCGCGGGGTGCGCACCTCGGAACGGGAGTACGAACTC GACTCCCTGGTGCTGGCGACCGGGTTCGACGCGCTGACCGGGGCGCTGTTCAAGATCGAC ATCCGCGGTGTGGGCAACGTCGCTTTGAAGGAGAAGTGGGCCGCAGGGCCACGCACCTAC CTGGGACTGTCCACCGCCGGGTTCCCCAACCTGTTCTTCATCGCAGGCCCGGGCAGCCCG TCTGCGCTCAGCAACATGCTGGTCTCTATCGAACAGCACGTGGAATGGGTGACCGACCAC ATCGCCTACATGTTCAAGAACGGCCTCACCCGATCCGAGGCAGTCCTGGAGAAAGAAGAC GAGTGGGTGGAGCACGTCAACGAGATCGCCGACGAAACCCTCTACCCCATGACCGCCTCG TGGTACACGGGCGCCAACGTCCCCGGTAAACCCCGGGTGTTCATGCTCTACGTCGGCGGC TTCCACCGCTACCGGCAGATCTGCGACGAGGTGGCTGCCAAGGGCTACGAAGGTTTCGTC CTCACCTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q47PU3 UniProtKB Entry Name PAMO_THEFY GenBank Gene ID CP000088 - General References
- Lykidis A, Mavromatis K, Ivanova N, Anderson I, Land M, DiBartolo G, Martinez M, Lapidus A, Lucas S, Copeland A, Richardson P, Wilson DB, Kyrpides N: Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX. J Bacteriol. 2007 Mar;189(6):2477-86. Epub 2007 Jan 5. [Article]
- Fraaije MW, Wu J, Heuts DP, van Hellemond EW, Spelberg JH, Janssen DB: Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining. Appl Microbiol Biotechnol. 2005 Jan;66(4):393-400. Epub 2004 Oct 27. [Article]
- Malito E, Alfieri A, Fraaije MW, Mattevi A: Crystal structure of a Baeyer-Villiger monooxygenase. Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13157-62. Epub 2004 Aug 24. [Article]
- Orru R, Dudek HM, Martinoli C, Torres Pazmino DE, Royant A, Weik M, Fraaije MW, Mattevi A: Snapshots of enzymatic Baeyer-Villiger catalysis: oxygen activation and intermediate stabilization. J Biol Chem. 2011 Aug 19;286(33):29284-91. doi: 10.1074/jbc.M111.255075. Epub 2011 Jun 22. [Article]
- Martinoli C, Dudek HM, Orru R, Edmondson DE, Fraaije MW, Mattevi A: Beyond the Protein Matrix: Probing Cofactor Variants in a Baeyer-Villiger Oxygenation Reaction. ACS Catal. 2013;3(12):3058-3062. [Article]