Trans-2,3-dihydro-3-hydroxyanthranilate isomerase
Details
- Name
- Trans-2,3-dihydro-3-hydroxyanthranilate isomerase
- Synonyms
- 5.3.3.17
- Phenazine/pyocyanine biosynthesis protein PhzF
- Gene Name
- phzF
- Organism
- Pseudomonas fluorescens
- Amino acid sequence
>lcl|BSEQ0022276|Trans-2,3-dihydro-3-hydroxyanthranilate isomerase MHNYVIIDAFASVPLEGNPVAVFFDADDLPPAQMQRIAREMNLSESTFVLKPRNGGDALI RIFTPVNELPFAGHPLLGTAIALGAHTDNHRLYLETQMGTIAFELERQNGSVIAASMDQP IPTWTALGRDAELLKALGISDSTFPIEIYHNGPRHVFVGLPSIDALSALHPDHRALSNFH DMAINCFAGAGRRWRSRMFSPAYGVVEDAATGSAAGPLAIHLARHGQIEFGQPVEILQGV EIGRPSLMFAKAEGRAEQLTRVEVSGNGVTFGRGTIVL
- Number of residues
- 278
- Molecular Weight
- 30052.965
- Theoretical pI
- 6.12
- GO Classification
- Functionsisomerase activityProcessespathogenesis / phenazine biosynthetic process
- General Function
- Isomerase activity
- Specific Function
- Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known.
- Pfam Domain Function
- PhzC-PhzF (PF02567)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0003073|837 bp ATGCACAACTACGTCATTATCGACGCCTTTGCCAGCGTCCCGCTGGAAGGCAATCCGGTC GCGGTGTTCTTTGACGCCGATGATTTACCGCCCGCGCAAATGCAGCGCATCGCCCGAGAA ATGAACCTCTCGGAGAGCACTTTTGTGCTCAAGCCGCGCAACGGTGGAGATGCGCTGATC CGGATTTTCACACCGGTCAACGAACTGCCTTTTGCCGGGCACCCGTTGCTGGGTACGGCC ATTGCCCTGGGAGCACATACCGACAACCACCGGCTGTATCTGGAAACCCAGATGGGCACC ATCGCCTTTGAACTGGAGCGCCAGAACGGCAGCGTTATCGCCGCCAGCATGGACCAGCCG ATCCCGACCTGGACGGCCCTGGGGCGTGACGCTGAACTGCTCAAGGCCTTGGGCATCAGT GATTCGACCTTTCCCATCGAGATCTATCACAACGGCCCGCGCCATGTGTTTGTCGGCCTG CCGAGCATTGACGCGTTATCGGCCCTGCACCCCGACCACCGTGCACTGTCCAACTTCCAT GACATGGCGATCAACTGTTTTGCCGGCGCAGGACGACGCTGGCGCAGCCGCATGTTCTCA CCGGCCTACGGCGTTGTGGAGGATGCGGCCACAGGCTCCGCCGCCGGACCATTGGCGATT CATCTGGCGCGCCATGGCCAGATTGAGTTTGGCCAGCCAGTCGAGATTTTACAGGGTGTG GAAATCGGCCGCCCCTCACTGATGTTCGCCAAAGCTGAGGGCCGCGCCGAGCAACTGACG CGAGTCGAGGTATCAGGTAATGGCGTTACATTTGGACGGGGGACCATCGTGCTATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q51792 UniProtKB Entry Name PHZF_PSEFL GenBank Protein ID 1045018 GenBank Gene ID L48616 - General References
- Mavrodi DV, Ksenzenko VN, Bonsall RF, Cook RJ, Boronin AM, Thomashow LS: A seven-gene locus for synthesis of phenazine-1-carboxylic acid by Pseudomonas fluorescens 2-79. J Bacteriol. 1998 May;180(9):2541-8. [Article]
- Ahuja EG, Janning P, Mentel M, Graebsch A, Breinbauer R, Hiller W, Costisella B, Thomashow LS, Mavrodi DV, Blankenfeldt W: PhzA/B catalyzes the formation of the tricycle in phenazine biosynthesis. J Am Chem Soc. 2008 Dec 17;130(50):17053-61. doi: 10.1021/ja806325k. [Article]
- Parsons JF, Song F, Parsons L, Calabrese K, Eisenstein E, Ladner JE: Structure and function of the phenazine biosynthesis protein PhzF from Pseudomonas fluorescens 2-79. Biochemistry. 2004 Oct 5;43(39):12427-35. [Article]
- Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV: Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens. Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. [Article]