Cholix toxin

Details

Name
Cholix toxin
Synonyms
  • 2.4.2.36
  • Exotoxin A
  • NAD(+)--diphthamide ADP-ribosyltransferase
  • toxA
Gene Name
chxA
Organism
Vibrio cholerae
Amino acid sequence
>lcl|BSEQ0012985|Cholix toxin
MYLTFYLEKVMKKMLLIAGATVISSMAHPTFAVEDELNIFDECRSPCSLTPEPGKPIQSK
LSIPSDVVLDEGVLYYSMTINDEQNDIKDEDKGESIITIGEFATVRATRHYVNQDAPFGV
IHLDITTENGTKTYSYNRKEGEFAINWLVPIGEDSPASIKISVDELDQQRNIIEVPKLYS
IDLDNQTLEQWKTQGNVSFSVTRPEHNIAISWPSVSYKAAQKEGSRHKRWAHWHTGLALC
WLVPMDAIYNYITQQNCTLGDNWFGGSYETVAGTPKVITVKQGIEQKPVEQRIHFSKGNA
MSALAAHRVCGVPLETLARSRKPRDLTDDLSCAYQAQNIVSLFVATRILFSHLDSVFTLN
LDEQEPEVAERLSDLRRINENNPGMVTQVLTVARQIYNDYVTHHPGLTPEQTSAGAQAAD
ILSLFCPDADKSCVASNNDQANINIESRSGRSYLPENRAVITPQGVTNWTYQELEATHQA
LTREGYVFVGYHGTNHVAAQTIVNRIAPVPRGNNTENEEKWGGLYVATHAEVAHGYARIK
EGTGEYGLPTRAERDARGVMLRVYIPRASLERFYRTNTPLENAEEHITQVIGHSLPLRNE
AFTGPESAGGEDETVIGWDMAIHAVAIPSTIPGNAYEELAIDEEAVAKEQSISTKPPYKE
RKDELK
Number of residues
666
Molecular Weight
74292.735
Theoretical pI
5.06
GO Classification
Functions
NAD+-diphthamide ADP-ribosyltransferase activity
General Function
Nad+-diphthamide adp-ribosyltransferase activity
Specific Function
An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ5EK40
UniProtKB Entry NameCHXA_VIBCL
GenBank Protein ID58615287
GenBank Gene IDAY876053
General References
  1. Purdy A, Rohwer F, Edwards R, Azam F, Bartlett DH: A glimpse into the expanded genome content of Vibrio cholerae through identification of genes present in environmental strains. J Bacteriol. 2005 May;187(9):2992-3001. [Article]
  2. Jorgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Merrill AR: Cholix toxin, a novel ADP-ribosylating factor from Vibrio cholerae. J Biol Chem. 2008 Apr 18;283(16):10671-8. doi: 10.1074/jbc.M710008200. Epub 2008 Feb 14. [Article]
  3. Turgeon Z, White D, Jorgensen R, Visschedyk D, Fieldhouse RJ, Mangroo D, Merrill AR: Yeast as a tool for characterizing mono-ADP-ribosyltransferase toxins. FEMS Microbiol Lett. 2009 Nov;300(1):97-106. doi: 10.1111/j.1574-6968.2009.01777.x. Epub 2009 Aug 31. [Article]
  4. Turgeon Z, Jorgensen R, Visschedyk D, Edwards PR, Legree S, McGregor C, Fieldhouse RJ, Mangroo D, Schapira M, Merrill AR: Newly discovered and characterized antivirulence compounds inhibit bacterial mono-ADP-ribosyltransferase toxins. Antimicrob Agents Chemother. 2011 Mar;55(3):983-91. doi: 10.1128/AAC.01164-10. Epub 2010 Dec 6. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB08348N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDEexperimentalunknownDetails