Cyclopropane mycolic acid synthase 3
Details
- Name
- Cyclopropane mycolic acid synthase 3
- Synonyms
- 2.1.1.79
- AdoMet-MT
- CFA synthase
- cma3
- cmaA3
- CMAS
- CMAS-3
- Cyclopropane fatty acid synthase
- Cyclopropane-fatty-acyl-phospholipid synthase
- MA-MT
- Mycolic acid methyltransferase
- S-adenosylmethionine-dependent methyltransferase
- SAM-MT
- Gene Name
- pcaA
- Organism
- Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- Amino acid sequence
>lcl|BSEQ0051138|Cyclopropane mycolic acid synthase 3 MSVQLTPHFGNVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKL NLEPGMTLLDIGCGWGATMRRAIEKYDVNVVGLTLSENQAGHVQKMFDQMDTPRSRRVLL EGWEKFDEPVDRIVSIGAFEHFGHQRYHHFFEVTHRTLPADGKMLLHTIVRPTFKEGREK GLTLTHELVHFTKFILAEIFPGGWLPSIPTVHEYAEKVGFRVTAVQSLQLHYARTLDMWA TALEANKDQAIAIQSQTVYDRYMKYLTGCAKLFRQGYTDVDQFTLEK
- Number of residues
- 287
- Molecular Weight
- 33027.5
- Theoretical pI
- Not Available
- GO Classification
- Functionscyclopropane-fatty-acyl-phospholipid synthase activityProcessesactive evasion of host immune response / modulation by symbiont of host innate immune response / mycolic acid biosynthetic process / pathogenesis / S-adenosylmethionine metabolic processComponentscytoplasm
- General Function
- Involved in the phagosome maturation block (PMB). Catalyzes the conversion of a double bond to a cyclopropane ring at the proximal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. It can use cis, cis 11,14-eicosadienoic acid and linoelaidic acid as substrate. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence.
- Specific Function
- Cyclopropane-fatty-acyl-phospholipid synthase activity
- Pfam Domain Function
- CMAS (PF02353)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0051139|Cyclopropane mycolic acid synthase 3 (pcaA) ATGTCCGTGCAGCTCACGCCGCATTTTGGAAACGTGCAAGCTCATTACGACCTCTCCGAC GACTTTTTCCGGCTGTTCTTGGACCCCACCCAGACCTACAGCTGTGCCTACTTCGAACGC GACGACATGACGCTGCAGGAGGCCCAGATCGCCAAGATCGACCTGGCCCTGGGCAAGCTG AACCTCGAACCCGGGATGACGTTGCTGGACATCGGCTGCGGCTGGGGCGCGACCATGCGG CGCGCCATCGAGAAATACGACGTCAATGTCGTGGGCCTGACGTTGTCGGAGAACCAGGCC GGTCATGTCCAGAAAATGTTCGACCAAATGGACACCCCCCGCTCCAGACGAGTGTTGCTG GAGGGATGGGAGAAATTTGACGAGCCCGTCGACCGCATCGTCTCGATCGGCGCGTTCGAG CACTTCGGCCACCAGCGCTACCACCATTTCTTCGAGGTGACCCACCGCACGTTGCCGGCC GACGGCAAGATGTTGCTGCACACCATCGTGCGCCCCACCTTCAAAGAAGGCAGGGAAAAG GGCCTGACGTTGACCCACGAACTGGTTCACTTCACCAAATTCATCCTGGCCGAGATCTTC CCCGGTGGCTGGCTGCCGTCCATCCCGACGGTGCACGAGTACGCCGAGAAGGTCGGCTTC CGGGTCACCGCGGTCCAGTCATTGCAGCTGCACTACGCCAGGACGCTGGACATGTGGGCC ACAGCGCTCGAGGCCAACAAAGATCAGGCCATCGCGATCCAGTCGCAGACTGTCTACGAC CGCTACATGAAGTACCTGACCGGCTGCGCGAAGCTGTTCCGCCAGGGTTACACCGACGTC GACCAGTTCACACTGGAAAAGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P9WPB3 UniProtKB Entry Name CMAS3_MYCTU - General References
- Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [Article]
- Glickman MS, Cox JS, Jacobs WR Jr: A novel mycolic acid cyclopropane synthetase is required for cording, persistence, and virulence of Mycobacterium tuberculosis. Mol Cell. 2000 Apr;5(4):717-27. [Article]
- Alahari A, Trivelli X, Guerardel Y, Dover LG, Besra GS, Sacchettini JC, Reynolds RC, Coxon GD, Kremer L: Thiacetazone, an antitubercular drug that inhibits cyclopropanation of cell wall mycolic acids in mycobacteria. PLoS One. 2007 Dec 19;2(12):e1343. [Article]
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- Vaubourgeix J, Bardou F, Boissier F, Julien S, Constant P, Ploux O, Daffe M, Quemard A, Mourey L: S-adenosyl-N-decyl-aminoethyl, a potent bisubstrate inhibitor of mycobacterium tuberculosis mycolic acid methyltransferases. J Biol Chem. 2009 Jul 17;284(29):19321-30. doi: 10.1074/jbc.M809599200. Epub 2009 May 13. [Article]
- Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [Article]
- Corrales RM, Molle V, Leiba J, Mourey L, de Chastellier C, Kremer L: Phosphorylation of mycobacterial PcaA inhibits mycolic acid cyclopropanation: consequences for intracellular survival and for phagosome maturation block. J Biol Chem. 2012 Jul 27;287(31):26187-99. doi: 10.1074/jbc.M112.373209. Epub 2012 May 23. [Article]
- Huang CC, Smith CV, Glickman MS, Jacobs WR Jr, Sacchettini JC: Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis. J Biol Chem. 2002 Mar 29;277(13):11559-69. Epub 2001 Dec 26. [Article]