Peptide deformylase
Details
- Name
- Peptide deformylase
- Synonyms
- 3.5.1.88
- Polypeptide deformylase
- Gene Name
- def
- Organism
- Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
- Amino acid sequence
>lcl|BSEQ0019482|Peptide deformylase MSVRKILRMGDPILRKISEPVTEDEIQTKEFKKLIRDMFDTMRHAEGVGLAAPQIGILKQ IVVVGSEDNERYPGTPDVPERIILNPVITPLTKDTSGFWEGCLSVPGMRGYVERPNQIRM QWMDEKGNQFDETIDGYKAIVYQHECDHLQGILYVDRLKDTKLFGFNETLDSSHNVLD
- Number of residues
- 178
- Molecular Weight
- 20379.185
- Theoretical pI
- 4.93
- GO Classification
- Functionsiron ion binding / peptide deformylase activityProcessestranslation
- General Function
- Peptide deformylase activity
- Specific Function
- Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).
- Pfam Domain Function
- Pep_deformylase (PF01327)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0019483|Peptide deformylase (def) ATGTCAGTCAGAAAAATTTTAAGAATGGGAGATCCAATTCTCCGTAAAATCTCAGAGCCA GTTACAGAAGACGAAATTCAAACTAAAGAATTCAAAAAGTTAATCCGAGATATGTTCGAT ACAATGCGACACGCGGAAGGAGTGGGACTTGCGGCTCCTCAGATCGGAATTTTGAAACAA ATCGTAGTCGTTGGTTCCGAAGATAATGAACGTTACCCAGGAACCCCAGACGTTCCAGAG AGAATCATTTTAAATCCAGTAATTACACCTCTTACAAAAGATACTTCCGGTTTTTGGGAA GGTTGCCTTTCCGTCCCAGGAATGCGCGGTTATGTGGAAAGACCCAACCAAATCAGAATG CAATGGATGGACGAAAAAGGAAACCAATTTGACGAAACCATAGACGGCTATAAAGCAATC GTCTATCAACATGAATGTGATCACCTACAGGGAATTTTATACGTAGATAGACTAAAAGAC ACTAAGTTATTCGGTTTTAACGAAACCCTAGACTCTTCTCACAACGTCCTAGACTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q93LE9 UniProtKB Entry Name DEF_LEPIN GenBank Protein ID 14626937 GenBank Gene ID AY040678 - General References
- Li Y, Ren S, Gong W: Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans. Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):846-8. Epub 2002 Apr 26. [Article]
- Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, Xu H, Zhang YX, Xiong H, Lu G, Lu LF, Jiang HQ, Jia J, Tu YF, Jiang JX, Gu WY, Zhang YQ, Cai Z, Sheng HH, Yin HF, Zhang Y, Zhu GF, Wan M, Huang HL, Qian Z, Wang SY, Ma W, Yao ZJ, Shen Y, Qiang BQ, Xia QC, Guo XK, Danchin A, Saint Girons I, Somerville RL, Wen YM, Shi MH, Chen Z, Xu JG, Zhao GP: Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature. 2003 Apr 24;422(6934):888-93. [Article]
- Zhou Z, Song X, Li Y, Gong W: Unique structural characteristics of peptide deformylase from pathogenic bacterium Leptospira interrogans. J Mol Biol. 2004 May 21;339(1):207-15. [Article]