Ribonuclease H
Details
- Name
- Ribonuclease H
- Synonyms
- 3.1.26.4
- RNase H
- Gene Name
- rnhA
- Organism
- Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
- Amino acid sequence
>lcl|BSEQ0017305|Ribonuclease H MAKSKYYVVWNGRKPGIYTSWSACEAQVKGYTGAKFKSYPSKEEAEAAFRGEEATPKLAK EEIIWESLSVDVGSQGNPGIVEYKGVDTKTGEVLFEREPIPIGTNNMGEFLAIVHGLRYL KERNSRKPIYSDSQTAIKWVKDKKAKSTLVRNEETALIWKLVDEAEEWLNTHTYETPILK WQTDKWGEIKADYGRK
- Number of residues
- 196
- Molecular Weight
- 22373.225
- Theoretical pI
- 8.81
- GO Classification
- Functionsmetal ion binding / nucleic acid binding / RNA-DNA hybrid ribonuclease activityComponentscytoplasm
- General Function
- Rna-dna hybrid ribonuclease activity
- Specific Function
- Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
- Pfam Domain Function
- Cauli_VI (PF01693)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0017306|Ribonuclease H (rnhA) ATGGCAAAGTCAAAATACTATGTCGTATGGAATGGACGGAAGCCCGGCATTTATACGAGC TGGTCTGCATGTGAAGCTCAAGTAAAAGGATATACCGGCGCCAAATTTAAATCCTATCCT TCCAAGGAAGAAGCAGAGGCTGCTTTTAGAGGAGAAGAGGCAACACCGAAGCTTGCAAAA GAGGAGATTATTTGGGAGAGCCTGTCTGTAGATGTTGGCAGCCAAGGGAATCCCGGAATT GTGGAATATAAAGGCGTTGATACGAAAACGGGAGAAGTCCTTTTTGAACGAGAGCCGATT CCGATCGGGACAAACAATATGGGTGAGTTTCTCGCGATCGTTCACGGGCTTCGTTACCTT AAGGAACGGAACAGTCGTAAGCCGATCTATTCTGATTCCCAGACGGCAATCAAATGGGTG AAGGATAAAAAAGCAAAATCGACCCTCGTGCGCAATGAAGAAACAGCGCTTATTTGGAAG CTTGTAGATGAAGCGGAGGAGTGGCTAAACACTCATACATATGAAACGCCCATCTTAAAA TGGCAGACCGATAAGTGGGGGGAAATTAAGGCCGATTACGGGAGAAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9KEI9 UniProtKB Entry Name RNH1_BACHD GenBank Protein ID 10172613 GenBank Gene ID BA000004 - General References
- Takami H, Nakasone K, Takaki Y, Maeno G, Sasaki R, Masui N, Fuji F, Hirama C, Nakamura Y, Ogasawara N, Kuhara S, Horikoshi K: Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res. 2000 Nov 1;28(21):4317-31. [Article]
- Nowotny M, Gaidamakov SA, Crouch RJ, Yang W: Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell. 2005 Jul 1;121(7):1005-16. [Article]