Inner centromere protein

Details

Name

Inner centromere protein

Synonyms

Not Available

Gene Name

INCENP

Organism

Humans

Amino acid sequence

>lcl|BSEQ0007304|Inner centromere protein
MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTP
SQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSV
LRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAE
QHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATP
QDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDS
QSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLE
RLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAAS
SPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPS
SPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQ
RLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIE
QKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRH
QELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLA
EQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKA
KEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRK
PIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSP
PLQGARVPSSLAYSLKKH

Number of residues

918

Molecular Weight

105427.925

Theoretical pI

10.1

GO Classification

Processes

chromosome segregation / cytokinesis / mitotic cell cycle / mitotic nuclear division / small GTPase mediated signal transduction

Components

central element / chromocenter / chromosome, centromeric region / condensed chromosome kinetochore / cytosol / kinetochore / lateral element / microtubule / midbody / pericentric heterochromatin / protein complex / spindle

General Function

Not Available

Specific Function

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.

Pfam Domain Function

• INCENP_ARK-bind (PF03941)
• INCENP_N (PF12178)

Transmembrane Regions

Not Available

Cellular Location

Nucleus

Gene sequence

>lcl|BSEQ0019470|Inner centromere protein (INCENP)
ATGGGGACGACGGCCCCAGGGCCCATTCACCTGCTGGAGCTATGTGACCAGAAGCTCATG
GAGTTTCTCTGCAACATGGATAATAAGGACTTGGTGTGGCTTGAGGAAATCCAAGAGGAG
GCCGAGCGCATGTTCACCAGAGAATTCAGCAAAGAGCCAGAGCTGATGCCCAAAACACCT
TCTCAGAAGAACCGACGGAAGAAGAGACGGATTTCTTATGTTCAGGATGAAAACAGAGAT
CCCATCAGGAGAAGGTTATCCCGCAGAAAGTCTCGGAGCAGCCAGCTGAGCTCCCGACGC
CTCCGCAGCAAGGACAGTGTAGAGAAGCTGGCTACAGTGGTCGGGGAGAACGGCTCCGTC
CTGCGGCGTGTGACCCGTGCTGCGGCTGCAGCTGCCGCGGCTACCATGGCATTGGCTGCA
CCTTCTTCACCCACCCCTGAGTCTCCCACGATGCTGACTAAGAAGCCCGAGGATAACCAC
ACCCAGTGCCAGCTGGTGCCTGTGGTGGAGATCGGCATCAGTGAGCGCCAGAATGCTGAG
CAGCATGTCACCCAGCTCATGTCCACCGAGCCTCTGCCCCGCACTCTGTCCCCGACTCCA
GCTTCAGCCACAGCTCCAACCTCCCAGGGCATCCCGACATCAGATGAGGAATCAACACCT
AAGAAGTCGAAGGCCAGGATACTGGAGTCCATCACAGTGAGCTCCCTGATGGCTACACCC
CAGGACCCCAAGGGTCAAGGGGTCGGGACGGGGCGGTCTGCGTCTAAGCTCAGGATTGCG
CAGGTCTCCCCTGGCCCACGGGACTCGCCAGCCTTTCCAGATTCTCCATGGCGGGAGCGG
GTGCTGGCTCCCATCCTGCCGGATAACTTCTCCACGCCCACGGGCTCTCGCACGGACTCT
CAATCGGTGCGGCACAGCCCGATCGCCCCGTCTTCCCCGAGTCCCCAAGTCTTAGCCCAG
AAGTACTCTCTGGTGGCCAAACAGGAAAGTGTTGTCCGCAGGGCGAGCAGAAGGCTTGCC
AAGAAGACTGCCGAAGAGCCAGCTGCCTCTGGCCGCATCATCTGTCACAGTTACCTGGAG
AGGCTCCTGAATGTTGAGGTGCCCCAGAAAGTTGGTTCTGAGCAGAAGGAACCCCCCGAG
GAGGCTGAGCCTGTGGCGGCAGCTGAGCCAGAGGTCCCTGAGAACAATGGAAATAACTCG
TGGCCCCACAATGACACGGAGATTGCCAACAGCACACCCAACCCGAAGCCTGCAGCCAGC
AGCCCGGAAACACCCTCTGCAGGGCAGCAAGAGGCCAAGACGGACCAAGCAGATGGACCC
AGAGAGCCACCGCAGAGTGCCAGGAGGAAGCGCAGCTACAAGCAGGCCGTGAGTGAGCTG
GACGAGGAGCAGCACCTGGAGGATGAGGAGCTGCAGCCCCCCAGGAGCAAGACCCCTTCC
TCACCCTGCCCAGCCAGCAAGGTGGTACGGCCCCTCCGGACCTTTCTGCACACAGTGCAG
AGGAACCAGATGCTCATGACCCCGACCTCAGCCCCACGCAGCGTCATGAAGTCCTTTATT
AAGCGCAACACTCCCCTGCGCATGGACCCCAAGTGCAGCTTCGTCGAGAAGGAGCGGCAG
CGCCTGGAGAATCTGCGGCGGAAGGAGGAGGCCGAGCAGCTGCGCAGGCAGAAGGTGGAG
GAGGACAAGCGGCGGCGGCTGGAGGAGGTGAAGCTGAAGCGTGAGGAACGCCTCCGCAAG
GTGCTGCAGGCCCGCGAGCGGGTGGAGCAGATGAAGGAGGAGAAGAAGAAGCAGATTGAG
CAGAAGTTTGCTCAGATCGACGAGAAGACTGAGAAGGCCAAGGAGGAGCGGCTGGCAGAG
GAGAAGGCCAAGAAAAAGGCGGCGGCCAAGAAGATGGAGGAGGTGGAAGCACGCAGGAAG
CAGGAAGAGGAGGCACGTAGGCTCAGGTGGCTGCAGCAGGAGGAGGAAGAGCGGCGGCAC
CAAGAGCTGCTGCAGAAGAAGAAGGAAGAGGAGCAGGAGCGGCTGCGGAAGGCGGCCGAG
GCTAAGCGGCTGGCAGAGCAGCGGGAGCAGGAGCGGCGGGAGCAGGAGCGGCGCGAGCAG
GAGCGGCGCGAGCAGGAGCGGCGGGAGCAGGAGCGGCGCGAGCAGGAGCGACAGCTGGCA
GAGCAGGAGCGTCGGCGGGAGCAGGAGCGGCTCCAGGCCGAGAGGGAGCTGCAGGAGCGG
GAGAAGGCCCTGCGGCTGCAGAAGGAGCAGCTGCAGAGGGAACTGGAGGAGAAGAAGAAG
AAGGAAGAGCAGCAGCGTCTGGCTGAGCGGCAGCTGCAGGAGGAGCAAGAGAAGAAAGCC
AAGGAGGCAGCAGGGGCCAGCAAGGCCCTGAATGTGACTGTGGACGTGCAGTCTCCAGCT
TGTACCTCATATCAGATGACTCCGCAAGGGCACAGGGCCCCTCCCAAGATCAACCCAGAT
AACTACGGGATGGATCTGAATAGCGACGACTCCACCGATGATGAGGCCCATCCCCGGAAG
CCCATCCCCACCTGGGCCCGAGGCACCCCGCTCAGCCAGGCTATCATTCACCAGTACTAC
CACCCACCGAACCTTCTGGAGCTCTTTGGAACCATTCTCCCACTGGACTTGGAGGATATC
TTCAAGAAGAGCAAGCCCCGCTATCACAAGCGCACCAGCTCTGCTGTCTGGAACTCACCG
CCCCTGCAGGGCGCCAGGGTCCCCAGCAGCCTGGCCTACAGCCTGAAGAAGCACTGA

Chromosome Location

11

Locus

11q12-q13

External Identifiers

Resource	Link
UniProtKB ID	Q9NQS7
UniProtKB Entry Name	INCE_HUMAN
GenBank Protein ID	9438179
GenBank Gene ID	AF282265
HGNC ID	HGNC:6058

General References

1. Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC: Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells. Chromosoma. 2001 May;110(2):65-74. [Article]
2. Li X, Sakashita G, Matsuzaki H, Sugimoto K, Kimura K, Hanaoka F, Taniguchi H, Furukawa K, Urano T: Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C. J Biol Chem. 2004 Nov 5;279(45):47201-11. Epub 2004 Aug 16. [Article]
3. Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, Lloyd C, Itoh T, Takeda T, Kim DW, She X, Barlow KF, Bloom T, Bruford E, Chang JL, Cuomo CA, Eichler E, FitzGerald MG, Jaffe DB, LaButti K, Nicol R, Park HS, Seaman C, Sougnez C, Yang X, Zimmer AR, Zody MC, Birren BW, Nusbaum C, Fujiyama A, Hattori M, Rogers J, Lander ES, Sakaki Y: Human chromosome 11 DNA sequence and analysis including novel gene identification. Nature. 2006 Mar 23;440(7083):497-500. [Article]
4. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
5. Ainsztein AM, Kandels-Lewis SE, Mackay AM, Earnshaw WC: INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1. J Cell Biol. 1998 Dec 28;143(7):1763-74. [Article]
6. Wheatley SP, Kandels-Lewis SE, Adams RR, Ainsztein AM, Earnshaw WC: INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow. Exp Cell Res. 2001 Jan 15;262(2):122-7. [Article]
7. Honda R, Korner R, Nigg EA: Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis. Mol Biol Cell. 2003 Aug;14(8):3325-41. Epub 2003 May 29. [Article]
8. Wheatley SP, Henzing AJ, Dodson H, Khaled W, Earnshaw WC: Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo. J Biol Chem. 2004 Feb 13;279(7):5655-60. Epub 2003 Nov 10. [Article]
9. Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC: Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J Cell Biol. 2004 Jul 19;166(2):179-91. Epub 2004 Jul 12. [Article]
10. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. [Article]
11. Faitar SL, Sossey-Alaoui K, Ranalli TA, Cowell JK: EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in the completion of cytokinesis. Exp Cell Res. 2006 Jul 15;312(12):2325-35. Epub 2006 Apr 19. [Article]
12. Qi W, Tang Z, Yu H: Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1. Mol Biol Cell. 2006 Aug;17(8):3705-16. Epub 2006 Jun 7. [Article]
13. Cantin GT, Yi W, Lu B, Park SK, Xu T, Lee JD, Yates JR 3rd: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res. 2008 Mar;7(3):1346-51. doi: 10.1021/pr0705441. Epub 2008 Jan 26. [Article]
14. Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Korner R, Greff Z, Keri G, Stemmann O, Mann M: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell. 2008 Aug 8;31(3):438-48. doi: 10.1016/j.molcel.2008.07.007. [Article]
15. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [Article]
16. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [Article]
17. Oppermann FS, Gnad F, Olsen JV, Hornberger R, Greff Z, Keri G, Mann M, Daub H: Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics. 2009 Jul;8(7):1751-64. doi: 10.1074/mcp.M800588-MCP200. Epub 2009 Apr 15. [Article]
18. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [Article]
19. Nozawa RS, Nagao K, Masuda HT, Iwasaki O, Hirota T, Nozaki N, Kimura H, Obuse C: Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation. Nat Cell Biol. 2010 Jul;12(7):719-27. doi: 10.1038/ncb2075. Epub 2010 Jun 20. [Article]
20. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. [Article]
21. Platica M, Ionescu A, Ivan E, Holland JF, Mandeli J, Platica O: PAR, a protein involved in the cell cycle, is functionally related to chromosomal passenger proteins. Int J Oncol. 2011 Mar;38(3):777-85. doi: 10.3892/ijo.2011.900. Epub 2011 Jan 11. [Article]
22. Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. [Article]
23. Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E: Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together. Cell. 2007 Oct 19;131(2):271-85. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB07340	Reversine	experimental	unknown		Details