Aminoglycoside N(6')-acetyltransferase type 1
Details
- Name
- Aminoglycoside N(6')-acetyltransferase type 1
- Synonyms
- 2.3.1.82
- AAC(6')-Iy
- Aminoglycoside resistance protein
- Gene Name
- Not Available
- Organism
- Salmonella enteritidis
- Amino acid sequence
>lcl|BSEQ0022293|Aminoglycoside N(6')-acetyltransferase type 1 MDIRQMNKTHLEHWRGLRKQLWPGHPDDAHLADGEEILQADHLASFIAMADGVAIGFADA SIRHDYVNGCDSSPVVFLEGIFVLPSFRQRGVAKQLIAAVQRWGTNKGCREMASDTSPEN TISQKVHQALGFEETERVIFYRKRC
- Number of residues
- 145
- Molecular Weight
- 16361.42
- Theoretical pI
- 6.88
- GO Classification
- Functionsaminoglycoside 6'-N-acetyltransferase activity / protein homodimerization activityProcessesacetyl-CoA metabolic process / histone acetylation / response to antibiotic
- General Function
- Protein homodimerization activity
- Specific Function
- Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, tobramycin, dibekacin and ribostamycin. Able to acetylate eukaryotic histone proteins.
- Pfam Domain Function
- Acetyltransf_1 (PF00583)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0005249|438 bp ATGGACATCAGGCAAATGAACAAAACCCATCTGGAGCACTGGCGCGGATTGCGAAAACAG CTCTGGCCTGGTCACCCGGATGACGCCCATCTGGCGGACGGCGAAGAAATTCTGCAAGCC GATCATCTGGCATCATTTATTGCGATGGCAGACGGGGTGGCGATTGGCTTTGCGGATGCC TCAATCCGCCACGATTATGTCAATGGCTGTGACAGTTCGCCCGTGGTTTTCCTTGAAGGT ATTTTTGTTCTCCCCTCATTCCGTCAACGCGGCGTAGCGAAACAATTGATTGCAGCGGTG CAACGATGGGGAACGAATAAAGGGTGTCGGGAAATGGCCTCCGATACCTCGCCGGAAAAT ACAATTTCCCAGAAAGTTCATCAGGCGTTAGGATTTGAGGAAACAGAGCGCGTCATTTTC TACCGAAAGCGTTGTTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9R381 UniProtKB Entry Name AAC6_SALEN GenBank Gene ID AF144881 - General References
- Magnet S, Courvalin P, Lambert T: Activation of the cryptic aac(6')-Iy aminoglycoside resistance gene of Salmonella by a chromosomal deletion generating a transcriptional fusion. J Bacteriol. 1999 Nov;181(21):6650-5. [Article]
- Vetting MW, Magnet S, Nieves E, Roderick SL, Blanchard JS: A bacterial acetyltransferase capable of regioselective N-acetylation of antibiotics and histones. Chem Biol. 2004 Apr;11(4):565-73. [Article]
- Magalhaes ML, Vetting MW, Gao F, Freiburger L, Auclair K, Blanchard JS: Kinetic and structural analysis of bisubstrate inhibition of the Salmonella enterica aminoglycoside 6'-N-acetyltransferase. Biochemistry. 2008 Jan 15;47(2):579-84. Epub 2007 Dec 21. [Article]