Carbapenam-3-carboxylate synthase
Details
- Name
- Carbapenam-3-carboxylate synthase
- Synonyms
- 6.3.3.6
- Carbapenam-3-carboxylate ligase
- Gene Name
- carA
- Organism
- Pectobacterium carotovorum subsp. carotovorum
- Amino acid sequence
>lcl|BSEQ0022483|Carbapenam-3-carboxylate synthase MSNSFCVVYKGSDTDINNIQRDFDGKGEALSNGYLFIEQNGHYQKCEMERGTAYLIGSLY NRTFLIGLAGVWEGEAYLANDAELLALLFTRLGANALALAEGDFCFFIDEPNGELTVITE SRGFSPVHVVQGKKAWMTNSLKLVTAAEGEGALWFEEEALVCQSLMRADTYTPVKNAQRL KPGAVHVLTHDSEGYSFVESRTLTTPASNQLLALPREPLLALIDRYLNAPLEDLAPRFDT VGIPLSGGLDSSLVTALASRHFKKLNTYSIGTELSNEFEFSQQVADALGTHHQMKILSET EVINGIIESIYYNEIFDGLSAEIQSGLFNVYRQAQGQVSCMLTGYGSDLLFGGILKPGAQ YDNPNQLLAEQVYRTRWTGEFATHGASCYGIDIRHPFWSHSLISLCHALHPDYKIFDNEV KNILREYADSLQLLPKDIVWRKKIGIHEGSSVNQAFANVLGSTVDNYQTKSRFTYRVYQA FLRGRLSITDVTPSQLKDLIKKD
- Number of residues
- 503
- Molecular Weight
- 55997.77
- Theoretical pI
- 5.21
- GO Classification
- Functionsasparagine synthase (glutamine-hydrolyzing) activity / ATP bindingProcessesantibiotic biosynthetic process / asparagine biosynthetic process
- General Function
- Atp binding
- Specific Function
- Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0005498|1512 bp GTGAGCAATAGTTTTTGCGTTGTTTATAAAGGTTCTGATACCGATATAAATAATATCCAA CGCGACTTCGACGGAAAGGGCGAAGCATTATCTAATGGCTATCTTTTTATCGAACAGAAT GGCCATTATCAGAAGTGTGAGATGGAAAGAGGAACGGCCTACCTGATAGGCTCGCTGTAC AATCGGACGTTTCTGATCGGATTGGCCGGTGTGTGGGAAGGCGAGGCTTATCTGGCAAAT GATGCCGAGCTGTTAGCGTTGCTGTTCACGCGTTTGGGAGCGAATGCACTGGCGCTGGCT GAAGGTGACTTCTGCTTTTTCATTGATGAACCAAACGGCGAATTGACGGTGATTACCGAG TCGCGTGGTTTCTCGCCGGTTCATGTCGTACAGGGCAAAAAAGCCTGGATGACCAATAGC CTTAAACTGGTTACTGCGGCAGAAGGTGAAGGCGCGCTGTGGTTTGAAGAAGAGGCGTTG GTGTGCCAGTCGCTGATGCGAGCGGATACCTATACGCCGGTGAAAAATGCGCAGCGTCTT AAGCCGGGAGCGGTGCATGTTCTTACGCACGATAGCGAAGGTTATTCTTTCGTTGAAAGC CGCACGCTGACCACACCAGCCAGCAACCAATTGTTAGCGCTCCCGCGTGAACCGCTGCTG GCATTGATTGATCGCTACCTTAATGCTCCGCTTGAGGATTTAGCGCCGCGCTTTGATACC GTAGGAATTCCCTTGTCAGGCGGTCTGGATTCCAGCCTGGTAACGGCGCTCGCCAGTCGT CATTTCAAAAAATTGAATACGTATTCGATTGGTACGGAACTCAGCAATGAGTTTGAGTTT TCTCAACAGGTTGCTGATGCACTCGGTACACATCATCAGATGAAAATTCTGTCCGAAACT GAAGTGATCAACGGCATCATCGAATCCATCTATTACAACGAAATATTTGACGGCTTATCC GCTGAAATCCAATCCGGGTTGTTCAATGTCTATCGTCAGGCTCAGGGGCAGGTGTCTTGC ATGCTCACCGGATATGGTTCCGACCTGCTCTTTGGCGGCATACTGAAACCAGGAGCGCAG TATGACAATCCGAATCAGCTGCTTGCCGAGCAAGTGTACCGGACGCGTTGGACAGGGGAG TTTGCTACCCACGGTGCTTCCTGTTACGGCATTGATATTCGCCACCCCTTCTGGAGCCAT TCCCTAATCTCTCTATGTCATGCGCTACATCCTGATTACAAAATTTTCGACAACGAAGTC AAAAACATCCTGCGTGAATACGCCGATTCGCTGCAATTGCTGCCGAAAGACATTGTCTGG CGCAAGAAAATCGGCATTCACGAAGGTTCCTCCGTCAATCAGGCCTTTGCGAATGTTCTC GGGTCAACGGTTGATAACTACCAGACCAAAAGTCGCTTTACCTACCGTGTTTATCAAGCC TTCCTTCGTGGCCGTCTCTCCATTACAGATGTGACGCCCTCTCAGCTTAAAGATCTGATT AAAAAGGATTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9XB61 UniProtKB Entry Name CARA_PECCC GenBank Gene ID U17224 - General References
- McGowan SJ, Sebaihia M, O'Leary S, Hardie KR, Williams P, Stewart GS, Bycroft BW, Salmond GP: Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel beta-lactam resistance mechanism. Mol Microbiol. 1997 Nov;26(3):545-56. [Article]
- Gerratana B, Stapon A, Townsend CA: Inhibition and alternate substrate studies on the mechanism of carbapenam synthetase from Erwinia carotovora. Biochemistry. 2003 Jul 1;42(25):7836-47. [Article]
- Arnett SO, Gerratana B, Townsend CA: Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase. Biochemistry. 2007 Aug 14;46(32):9337-45. Epub 2007 Jul 21. [Article]
- Raber ML, Arnett SO, Townsend CA: A conserved tyrosyl-glutamyl catalytic dyad in evolutionarily linked enzymes: carbapenam synthetase and beta-lactam synthetase. Biochemistry. 2009 Jun 9;48(22):4959-71. doi: 10.1021/bi900432n. [Article]
- Miller MT, Gerratana B, Stapon A, Townsend CA, Rosenzweig AC: Crystal structure of carbapenam synthetase (CarA). J Biol Chem. 2003 Oct 17;278(42):40996-1002. Epub 2003 Jul 30. [Article]